Weissella soli
Average proteome isoelectric point is 6.25
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1537 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A288Q6M8|A0A288Q6M8_9LACO Molecular chaperone DnaJ OS=Weissella soli OX=155866 GN=DFP99_0717 PE=4 SV=1
MM1 pKa = 7.41 KK2 pKa = 10.07 KK3 pKa = 10.25 VKK5 pKa = 10.22 AVLSGTVAAAAATLVSTTASADD27 pKa = 3.56 TYY29 pKa = 11.04 DD30 pKa = 3.72 YY31 pKa = 11.26 TVVQGDD37 pKa = 4.44 TLWHH41 pKa = 6.31 LAAVTNDD48 pKa = 3.25 TVEE51 pKa = 4.54 NIAARR56 pKa = 11.84 NAIDD60 pKa = 4.19 NPNLIYY66 pKa = 9.92 IDD68 pKa = 3.6 QVVTLDD74 pKa = 3.54 SAATTSVATSVATSSAAATVASDD97 pKa = 3.97 TVDD100 pKa = 3.35 SAALASSAAASLAAASSAATTEE122 pKa = 4.21 SAALASSAAASLAAASSAATEE143 pKa = 4.26 SAALASSVAASSAAEE158 pKa = 4.14 SAALASSEE166 pKa = 4.12 AASLAAASSAVAEE179 pKa = 4.56 SVTEE183 pKa = 4.2 SAALASSEE191 pKa = 4.13 AASAAAEE198 pKa = 4.24 SAALASSEE206 pKa = 4.08 AASLATASSAATEE219 pKa = 4.26 SAALASSEE227 pKa = 4.05 AAASSAAASSAVASAAAASSAATSIANSVAASKK260 pKa = 10.25 AASSAASVAAASSAAASVAAVEE282 pKa = 4.55 AAQSASTEE290 pKa = 4.31 EE291 pKa = 4.23 VASTSSVSGSGSVYY305 pKa = 10.69 DD306 pKa = 3.47 QFIAAGGTDD315 pKa = 4.77 LLWKK319 pKa = 10.37 YY320 pKa = 10.36 IVLPEE325 pKa = 4.58 SGGNPNAVSPNGYY338 pKa = 9.51 HH339 pKa = 7.51 GLGQTMEE346 pKa = 3.81 SWGYY350 pKa = 11.24 GSVATQTAGLVNYY363 pKa = 9.6 AVSRR367 pKa = 11.84 YY368 pKa = 9.78 GSIDD372 pKa = 3.12 AAVTFRR378 pKa = 11.84 QANGWWW384 pKa = 3.24
Molecular weight: 36.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.78
IPC2_protein 3.948
IPC_protein 3.884
Toseland 3.706
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.745
Rodwell 3.719
Grimsley 3.617
Solomon 3.846
Lehninger 3.795
Nozaki 3.973
DTASelect 4.126
Thurlkill 3.732
EMBOSS 3.757
Sillero 3.999
Patrickios 1.875
IPC_peptide 3.846
IPC2_peptide 3.986
IPC2.peptide.svr19 3.913
Protein with the highest isoelectric point:
>tr|A0A288QYD8|A0A288QYD8_9LACO Uncharacterized protein OS=Weissella soli OX=155866 GN=DFP99_1367 PE=4 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 9.69 QPKK8 pKa = 8.34 KK9 pKa = 7.47 RR10 pKa = 11.84 HH11 pKa = 5.71 RR12 pKa = 11.84 EE13 pKa = 3.7 RR14 pKa = 11.84 VHH16 pKa = 6.23 GFRR19 pKa = 11.84 KK20 pKa = 10.04 RR21 pKa = 11.84 MSTSNGRR28 pKa = 11.84 KK29 pKa = 8.01 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 QKK37 pKa = 10.39 GRR39 pKa = 11.84 KK40 pKa = 8.52 VLSAA44 pKa = 4.05
Molecular weight: 5.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.335
IPC2_protein 10.862
IPC_protein 12.281
Toseland 12.442
ProMoST 12.925
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.193
Grimsley 12.486
Solomon 12.925
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 11.93
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.043
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1537
0
1537
484907
32
1771
315.5
34.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.735 ± 0.079
0.113 ± 0.008
5.736 ± 0.058
5.702 ± 0.071
4.257 ± 0.047
6.946 ± 0.055
2.122 ± 0.032
6.902 ± 0.057
5.239 ± 0.066
9.735 ± 0.075
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.788 ± 0.03
4.295 ± 0.044
3.548 ± 0.031
4.445 ± 0.051
4.205 ± 0.051
5.318 ± 0.06
6.604 ± 0.052
7.779 ± 0.054
1.157 ± 0.026
3.374 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here