Amino acid dipepetide frequency for Klebsiella phage Henu1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.554AlaAla: 9.554 ± 1.18
0.682AlaCys: 0.682 ± 0.261
6.398AlaAsp: 6.398 ± 0.739
5.63AlaGlu: 5.63 ± 0.592
3.668AlaPhe: 3.668 ± 0.523
7.08AlaGly: 7.08 ± 1.157
1.45AlaHis: 1.45 ± 0.322
4.095AlaIle: 4.095 ± 0.412
5.886AlaLys: 5.886 ± 1.085
8.189AlaLeu: 8.189 ± 1.077
2.815AlaMet: 2.815 ± 0.683
4.18AlaAsn: 4.18 ± 0.421
3.412AlaPro: 3.412 ± 0.513
3.668AlaGln: 3.668 ± 0.659
5.118AlaArg: 5.118 ± 0.785
5.801AlaSer: 5.801 ± 1.009
3.583AlaThr: 3.583 ± 0.443
5.459AlaVal: 5.459 ± 0.522
1.365AlaTrp: 1.365 ± 0.351
3.071AlaTyr: 3.071 ± 0.52
0.085AlaXaa: 0.085 ± 0.083
Cys
0.682CysAla: 0.682 ± 0.31
0.085CysCys: 0.085 ± 0.086
0.427CysAsp: 0.427 ± 0.277
1.024CysGlu: 1.024 ± 0.368
0.427CysPhe: 0.427 ± 0.223
0.853CysGly: 0.853 ± 0.258
0.171CysHis: 0.171 ± 0.122
0.768CysIle: 0.768 ± 0.275
0.682CysLys: 0.682 ± 0.268
0.853CysLeu: 0.853 ± 0.288
0.0CysMet: 0.0 ± 0.0
0.341CysAsn: 0.341 ± 0.146
0.682CysPro: 0.682 ± 0.22
0.512CysGln: 0.512 ± 0.27
0.682CysArg: 0.682 ± 0.315
0.427CysSer: 0.427 ± 0.183
0.512CysThr: 0.512 ± 0.214
0.682CysVal: 0.682 ± 0.212
0.171CysTrp: 0.171 ± 0.141
0.341CysTyr: 0.341 ± 0.177
0.0CysXaa: 0.0 ± 0.0
Asp
6.142AspAla: 6.142 ± 0.734
0.427AspCys: 0.427 ± 0.224
4.692AspAsp: 4.692 ± 0.618
3.753AspGlu: 3.753 ± 0.578
2.388AspPhe: 2.388 ± 0.485
6.142AspGly: 6.142 ± 0.564
0.853AspHis: 0.853 ± 0.279
3.071AspIle: 3.071 ± 0.451
4.692AspLys: 4.692 ± 0.661
3.753AspLeu: 3.753 ± 0.612
2.133AspMet: 2.133 ± 0.369
1.962AspAsn: 1.962 ± 0.337
2.986AspPro: 2.986 ± 0.516
2.559AspGln: 2.559 ± 0.5
2.815AspArg: 2.815 ± 0.396
2.73AspSer: 2.73 ± 0.416
4.009AspThr: 4.009 ± 0.542
4.862AspVal: 4.862 ± 0.476
1.024AspTrp: 1.024 ± 0.308
2.303AspTyr: 2.303 ± 0.338
0.0AspXaa: 0.0 ± 0.0
Glu
7.336GluAla: 7.336 ± 0.894
0.682GluCys: 0.682 ± 0.279
4.009GluAsp: 4.009 ± 0.729
5.203GluGlu: 5.203 ± 0.871
2.559GluPhe: 2.559 ± 0.374
5.801GluGly: 5.801 ± 0.803
1.535GluHis: 1.535 ± 0.524
2.73GluIle: 2.73 ± 0.399
2.986GluLys: 2.986 ± 0.608
6.398GluLeu: 6.398 ± 0.951
1.365GluMet: 1.365 ± 0.568
2.218GluAsn: 2.218 ± 0.337
2.303GluPro: 2.303 ± 0.577
3.241GluGln: 3.241 ± 0.804
4.606GluArg: 4.606 ± 0.822
4.35GluSer: 4.35 ± 0.575
2.9GluThr: 2.9 ± 0.54
4.095GluVal: 4.095 ± 0.423
0.512GluTrp: 0.512 ± 0.192
3.156GluTyr: 3.156 ± 0.415
0.0GluXaa: 0.0 ± 0.0
Phe
2.303PheAla: 2.303 ± 0.337
0.341PheCys: 0.341 ± 0.198
2.815PheAsp: 2.815 ± 0.535
1.962PheGlu: 1.962 ± 0.449
1.024PhePhe: 1.024 ± 0.276
3.241PheGly: 3.241 ± 0.679
0.938PheHis: 0.938 ± 0.311
1.962PheIle: 1.962 ± 0.472
2.218PheLys: 2.218 ± 0.405
2.986PheLeu: 2.986 ± 0.435
1.024PheMet: 1.024 ± 0.293
2.388PheAsn: 2.388 ± 0.45
1.365PhePro: 1.365 ± 0.367
1.535PheGln: 1.535 ± 0.345
1.962PheArg: 1.962 ± 0.358
2.133PheSer: 2.133 ± 0.407
2.218PheThr: 2.218 ± 0.352
2.047PheVal: 2.047 ± 0.393
0.341PheTrp: 0.341 ± 0.167
0.938PheTyr: 0.938 ± 0.2
0.0PheXaa: 0.0 ± 0.0
Gly
7.336GlyAla: 7.336 ± 0.931
0.938GlyCys: 0.938 ± 0.385
5.374GlyAsp: 5.374 ± 0.622
5.63GlyGlu: 5.63 ± 0.683
2.73GlyPhe: 2.73 ± 0.437
6.568GlyGly: 6.568 ± 0.881
1.365GlyHis: 1.365 ± 0.347
5.033GlyIle: 5.033 ± 0.884
6.056GlyLys: 6.056 ± 0.863
6.142GlyLeu: 6.142 ± 0.681
2.303GlyMet: 2.303 ± 0.44
2.986GlyAsn: 2.986 ± 0.519
1.45GlyPro: 1.45 ± 0.39
3.156GlyGln: 3.156 ± 0.594
4.948GlyArg: 4.948 ± 0.594
6.398GlySer: 6.398 ± 0.681
4.436GlyThr: 4.436 ± 0.584
4.777GlyVal: 4.777 ± 0.788
1.621GlyTrp: 1.621 ± 0.423
3.412GlyTyr: 3.412 ± 0.557
0.0GlyXaa: 0.0 ± 0.0
His
1.28HisAla: 1.28 ± 0.359
0.427HisCys: 0.427 ± 0.172
1.365HisAsp: 1.365 ± 0.331
1.621HisGlu: 1.621 ± 0.354
0.768HisPhe: 0.768 ± 0.241
1.706HisGly: 1.706 ± 0.423
0.682HisHis: 0.682 ± 0.244
0.853HisIle: 0.853 ± 0.319
0.938HisLys: 0.938 ± 0.226
1.28HisLeu: 1.28 ± 0.401
0.512HisMet: 0.512 ± 0.205
0.427HisAsn: 0.427 ± 0.173
0.682HisPro: 0.682 ± 0.198
0.427HisGln: 0.427 ± 0.19
0.597HisArg: 0.597 ± 0.202
1.024HisSer: 1.024 ± 0.279
0.938HisThr: 0.938 ± 0.204
1.791HisVal: 1.791 ± 0.386
0.171HisTrp: 0.171 ± 0.116
0.768HisTyr: 0.768 ± 0.175
0.0HisXaa: 0.0 ± 0.0
Ile
4.095IleAla: 4.095 ± 0.533
0.597IleCys: 0.597 ± 0.191
2.986IleAsp: 2.986 ± 0.421
3.497IleGlu: 3.497 ± 0.556
1.109IlePhe: 1.109 ± 0.265
3.753IleGly: 3.753 ± 0.624
0.768IleHis: 0.768 ± 0.261
2.474IleIle: 2.474 ± 0.461
3.583IleLys: 3.583 ± 0.539
3.071IleLeu: 3.071 ± 0.405
1.28IleMet: 1.28 ± 0.344
2.388IleAsn: 2.388 ± 0.644
2.388IlePro: 2.388 ± 0.469
1.535IleGln: 1.535 ± 0.329
3.753IleArg: 3.753 ± 0.557
2.9IleSer: 2.9 ± 0.41
3.839IleThr: 3.839 ± 0.829
2.986IleVal: 2.986 ± 0.511
0.597IleTrp: 0.597 ± 0.191
1.621IleTyr: 1.621 ± 0.371
0.0IleXaa: 0.0 ± 0.0
Lys
8.36LysAla: 8.36 ± 1.505
0.341LysCys: 0.341 ± 0.174
3.668LysAsp: 3.668 ± 0.695
4.521LysGlu: 4.521 ± 0.741
2.644LysPhe: 2.644 ± 0.424
6.312LysGly: 6.312 ± 0.865
1.535LysHis: 1.535 ± 0.365
2.303LysIle: 2.303 ± 0.518
3.497LysLys: 3.497 ± 0.856
5.203LysLeu: 5.203 ± 0.711
1.535LysMet: 1.535 ± 0.326
2.303LysAsn: 2.303 ± 0.41
2.9LysPro: 2.9 ± 0.602
1.962LysGln: 1.962 ± 0.415
3.668LysArg: 3.668 ± 0.66
3.583LysSer: 3.583 ± 0.586
2.815LysThr: 2.815 ± 0.45
5.203LysVal: 5.203 ± 0.751
0.682LysTrp: 0.682 ± 0.225
1.962LysTyr: 1.962 ± 0.402
0.085LysXaa: 0.085 ± 0.118
Leu
7.421LeuAla: 7.421 ± 1.165
0.341LeuCys: 0.341 ± 0.167
4.692LeuAsp: 4.692 ± 0.744
6.227LeuGlu: 6.227 ± 0.944
2.388LeuPhe: 2.388 ± 0.544
5.118LeuGly: 5.118 ± 0.657
1.28LeuHis: 1.28 ± 0.269
3.156LeuIle: 3.156 ± 0.529
6.056LeuLys: 6.056 ± 0.717
5.63LeuLeu: 5.63 ± 0.824
2.218LeuMet: 2.218 ± 0.363
4.35LeuAsn: 4.35 ± 0.657
2.986LeuPro: 2.986 ± 0.477
3.497LeuGln: 3.497 ± 0.582
4.521LeuArg: 4.521 ± 0.605
4.777LeuSer: 4.777 ± 0.668
5.289LeuThr: 5.289 ± 0.808
5.033LeuVal: 5.033 ± 0.628
1.194LeuTrp: 1.194 ± 0.307
2.303LeuTyr: 2.303 ± 0.418
0.0LeuXaa: 0.0 ± 0.0
Met
3.156MetAla: 3.156 ± 0.515
0.256MetCys: 0.256 ± 0.154
2.047MetAsp: 2.047 ± 0.436
1.28MetGlu: 1.28 ± 0.334
0.682MetPhe: 0.682 ± 0.263
1.791MetGly: 1.791 ± 0.309
0.512MetHis: 0.512 ± 0.256
1.109MetIle: 1.109 ± 0.27
1.365MetLys: 1.365 ± 0.325
2.9MetLeu: 2.9 ± 0.549
0.682MetMet: 0.682 ± 0.248
1.024MetAsn: 1.024 ± 0.207
0.682MetPro: 0.682 ± 0.221
2.047MetGln: 2.047 ± 0.494
0.853MetArg: 0.853 ± 0.259
1.962MetSer: 1.962 ± 0.411
1.706MetThr: 1.706 ± 0.384
1.28MetVal: 1.28 ± 0.39
0.0MetTrp: 0.0 ± 0.0
0.682MetTyr: 0.682 ± 0.254
0.0MetXaa: 0.0 ± 0.0
Asn
3.753AsnAla: 3.753 ± 0.698
0.853AsnCys: 0.853 ± 0.316
1.962AsnAsp: 1.962 ± 0.333
2.388AsnGlu: 2.388 ± 0.551
1.535AsnPhe: 1.535 ± 0.385
4.692AsnGly: 4.692 ± 0.681
0.427AsnHis: 0.427 ± 0.177
2.73AsnIle: 2.73 ± 0.501
2.133AsnLys: 2.133 ± 0.369
2.474AsnLeu: 2.474 ± 0.426
0.682AsnMet: 0.682 ± 0.288
1.791AsnAsn: 1.791 ± 0.356
2.133AsnPro: 2.133 ± 0.338
1.28AsnGln: 1.28 ± 0.254
1.706AsnArg: 1.706 ± 0.511
2.815AsnSer: 2.815 ± 0.43
2.133AsnThr: 2.133 ± 0.424
3.241AsnVal: 3.241 ± 0.57
0.597AsnTrp: 0.597 ± 0.249
2.133AsnTyr: 2.133 ± 0.457
0.0AsnXaa: 0.0 ± 0.0
Pro
2.815ProAla: 2.815 ± 0.475
0.512ProCys: 0.512 ± 0.245
2.218ProAsp: 2.218 ± 0.368
4.35ProGlu: 4.35 ± 0.851
1.706ProPhe: 1.706 ± 0.317
2.644ProGly: 2.644 ± 0.437
0.512ProHis: 0.512 ± 0.191
1.194ProIle: 1.194 ± 0.392
2.73ProLys: 2.73 ± 0.484
2.559ProLeu: 2.559 ± 0.44
0.768ProMet: 0.768 ± 0.289
2.218ProAsn: 2.218 ± 0.435
1.194ProPro: 1.194 ± 0.369
1.621ProGln: 1.621 ± 0.328
1.877ProArg: 1.877 ± 0.459
1.706ProSer: 1.706 ± 0.331
1.791ProThr: 1.791 ± 0.406
2.815ProVal: 2.815 ± 0.377
0.768ProTrp: 0.768 ± 0.222
1.706ProTyr: 1.706 ± 0.35
0.0ProXaa: 0.0 ± 0.0
Gln
3.497GlnAla: 3.497 ± 0.555
0.085GlnCys: 0.085 ± 0.104
2.73GlnAsp: 2.73 ± 0.35
2.73GlnGlu: 2.73 ± 0.429
1.706GlnPhe: 1.706 ± 0.269
3.156GlnGly: 3.156 ± 0.462
0.427GlnHis: 0.427 ± 0.197
1.706GlnIle: 1.706 ± 0.486
2.9GlnLys: 2.9 ± 0.465
4.009GlnLeu: 4.009 ± 0.506
1.706GlnMet: 1.706 ± 0.564
1.365GlnAsn: 1.365 ± 0.306
1.706GlnPro: 1.706 ± 0.318
3.241GlnGln: 3.241 ± 0.681
2.388GlnArg: 2.388 ± 0.634
2.73GlnSer: 2.73 ± 0.558
1.621GlnThr: 1.621 ± 0.489
2.9GlnVal: 2.9 ± 0.459
0.682GlnTrp: 0.682 ± 0.282
1.45GlnTyr: 1.45 ± 0.496
0.0GlnXaa: 0.0 ± 0.0
Arg
4.862ArgAla: 4.862 ± 0.788
0.853ArgCys: 0.853 ± 0.293
3.497ArgAsp: 3.497 ± 0.568
4.009ArgGlu: 4.009 ± 0.619
1.535ArgPhe: 1.535 ± 0.342
4.095ArgGly: 4.095 ± 0.583
0.938ArgHis: 0.938 ± 0.262
3.583ArgIle: 3.583 ± 0.54
3.753ArgLys: 3.753 ± 0.745
4.436ArgLeu: 4.436 ± 0.625
1.28ArgMet: 1.28 ± 0.298
2.218ArgAsn: 2.218 ± 0.389
2.133ArgPro: 2.133 ± 0.317
2.644ArgGln: 2.644 ± 0.553
3.071ArgArg: 3.071 ± 0.396
3.668ArgSer: 3.668 ± 0.464
3.241ArgThr: 3.241 ± 0.476
3.583ArgVal: 3.583 ± 0.504
1.194ArgTrp: 1.194 ± 0.357
1.45ArgTyr: 1.45 ± 0.309
0.0ArgXaa: 0.0 ± 0.0
Ser
5.203SerAla: 5.203 ± 0.809
1.024SerCys: 1.024 ± 0.306
4.692SerAsp: 4.692 ± 0.679
3.497SerGlu: 3.497 ± 0.571
2.986SerPhe: 2.986 ± 0.514
5.033SerGly: 5.033 ± 0.656
1.45SerHis: 1.45 ± 0.319
2.73SerIle: 2.73 ± 0.45
4.521SerLys: 4.521 ± 0.549
4.436SerLeu: 4.436 ± 0.692
1.621SerMet: 1.621 ± 0.396
1.962SerAsn: 1.962 ± 0.468
1.962SerPro: 1.962 ± 0.333
2.474SerGln: 2.474 ± 0.527
3.924SerArg: 3.924 ± 0.614
3.583SerSer: 3.583 ± 0.548
3.839SerThr: 3.839 ± 0.606
4.35SerVal: 4.35 ± 0.837
0.853SerTrp: 0.853 ± 0.222
2.303SerTyr: 2.303 ± 0.546
0.0SerXaa: 0.0 ± 0.0
Thr
4.521ThrAla: 4.521 ± 0.691
0.938ThrCys: 0.938 ± 0.296
2.9ThrAsp: 2.9 ± 0.397
3.668ThrGlu: 3.668 ± 0.538
2.047ThrPhe: 2.047 ± 0.422
4.777ThrGly: 4.777 ± 0.755
1.109ThrHis: 1.109 ± 0.22
4.095ThrIle: 4.095 ± 0.585
4.009ThrLys: 4.009 ± 0.595
5.118ThrLeu: 5.118 ± 0.737
1.28ThrMet: 1.28 ± 0.294
1.877ThrAsn: 1.877 ± 0.365
2.9ThrPro: 2.9 ± 0.43
2.303ThrGln: 2.303 ± 0.485
2.218ThrArg: 2.218 ± 0.395
3.583ThrSer: 3.583 ± 0.621
2.986ThrThr: 2.986 ± 0.697
3.327ThrVal: 3.327 ± 0.668
0.597ThrTrp: 0.597 ± 0.207
1.28ThrTyr: 1.28 ± 0.306
0.0ThrXaa: 0.0 ± 0.0
Val
5.63ValAla: 5.63 ± 0.733
0.427ValCys: 0.427 ± 0.175
3.583ValAsp: 3.583 ± 0.511
4.265ValGlu: 4.265 ± 0.597
2.218ValPhe: 2.218 ± 0.586
5.971ValGly: 5.971 ± 0.806
0.938ValHis: 0.938 ± 0.301
3.668ValIle: 3.668 ± 0.592
4.009ValLys: 4.009 ± 0.5
5.374ValLeu: 5.374 ± 0.655
1.365ValMet: 1.365 ± 0.325
2.986ValAsn: 2.986 ± 0.701
2.388ValPro: 2.388 ± 0.505
2.73ValGln: 2.73 ± 0.409
4.095ValArg: 4.095 ± 0.616
5.033ValSer: 5.033 ± 1.048
4.692ValThr: 4.692 ± 0.604
4.948ValVal: 4.948 ± 0.801
0.597ValTrp: 0.597 ± 0.311
2.474ValTyr: 2.474 ± 0.424
0.0ValXaa: 0.0 ± 0.0
Trp
0.682TrpAla: 0.682 ± 0.229
0.341TrpCys: 0.341 ± 0.152
0.597TrpAsp: 0.597 ± 0.233
1.024TrpGlu: 1.024 ± 0.305
0.341TrpPhe: 0.341 ± 0.159
0.512TrpGly: 0.512 ± 0.21
0.341TrpHis: 0.341 ± 0.212
0.512TrpIle: 0.512 ± 0.294
1.194TrpLys: 1.194 ± 0.415
1.45TrpLeu: 1.45 ± 0.368
0.341TrpMet: 0.341 ± 0.171
0.682TrpAsn: 0.682 ± 0.232
0.256TrpPro: 0.256 ± 0.133
0.597TrpGln: 0.597 ± 0.244
1.024TrpArg: 1.024 ± 0.312
1.109TrpSer: 1.109 ± 0.385
0.768TrpThr: 0.768 ± 0.246
1.535TrpVal: 1.535 ± 0.415
0.341TrpTrp: 0.341 ± 0.155
0.171TrpTyr: 0.171 ± 0.114
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.474TyrAla: 2.474 ± 0.504
0.171TyrCys: 0.171 ± 0.136
2.644TyrAsp: 2.644 ± 0.537
1.706TyrGlu: 1.706 ± 0.36
1.28TyrPhe: 1.28 ± 0.302
3.327TyrGly: 3.327 ± 0.447
1.024TyrHis: 1.024 ± 0.405
1.45TyrIle: 1.45 ± 0.302
1.877TyrLys: 1.877 ± 0.336
2.218TyrLeu: 2.218 ± 0.396
1.024TyrMet: 1.024 ± 0.28
1.791TyrAsn: 1.791 ± 0.447
1.194TyrPro: 1.194 ± 0.272
1.791TyrGln: 1.791 ± 0.436
2.218TyrArg: 2.218 ± 0.402
2.133TyrSer: 2.133 ± 0.537
2.218TyrThr: 2.218 ± 0.391
2.474TyrVal: 2.474 ± 0.576
0.512TyrTrp: 0.512 ± 0.199
0.938TyrTyr: 0.938 ± 0.354
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.085XaaAla: 0.085 ± 0.083
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.085XaaGly: 0.085 ± 0.118
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 42 proteins (11724 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski