Streptococcus satellite phage Javan412
Average proteome isoelectric point is 6.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 19 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZM55|A0A4D5ZM55_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan412 OX=2558688 GN=JavanS412_0009 PE=4 SV=1
MM1 pKa = 7.6 KK2 pKa = 10.4 NLEE5 pKa = 4.17 EE6 pKa = 5.44 KK7 pKa = 10.32 IEE9 pKa = 4.01 PVVIILDD16 pKa = 3.82 EE17 pKa = 4.39 EE18 pKa = 4.81 DD19 pKa = 5.15 DD20 pKa = 4.1 FDD22 pKa = 5.15 FEE24 pKa = 4.31 EE25 pKa = 4.61 TEE27 pKa = 4.09 EE28 pKa = 4.39 EE29 pKa = 3.89 IQAEE33 pKa = 4.38 YY34 pKa = 10.29 EE35 pKa = 4.27 AQCLADD41 pKa = 4.06 DD42 pKa = 4.22 TQDD45 pKa = 3.19 IINWINEE52 pKa = 4.2 SNTLAEE58 pKa = 4.29 YY59 pKa = 11.21 VSMEE63 pKa = 4.26 FEE65 pKa = 4.68 NMNKK69 pKa = 10.05 DD70 pKa = 3.55 DD71 pKa = 5.35 LGASLEE77 pKa = 4.2 NASQCLDD84 pKa = 4.41 AIVKK88 pKa = 8.99 MLEE91 pKa = 3.74 NEE93 pKa = 4.14 TLILKK98 pKa = 10.22 GGSNEE103 pKa = 3.92 NN104 pKa = 3.38
Molecular weight: 11.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.224
IPC2_protein 3.567
IPC_protein 3.503
Toseland 3.325
ProMoST 3.656
Dawson 3.478
Bjellqvist 3.643
Wikipedia 3.376
Rodwell 3.338
Grimsley 3.236
Solomon 3.452
Lehninger 3.401
Nozaki 3.605
DTASelect 3.719
Thurlkill 3.376
EMBOSS 3.389
Sillero 3.617
Patrickios 3.045
IPC_peptide 3.452
IPC2_peptide 3.592
IPC2.peptide.svr19 3.658
Protein with the highest isoelectric point:
>tr|A0A4D5ZPE3|A0A4D5ZPE3_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan412 OX=2558688 GN=JavanS412_0013 PE=4 SV=1
MM1 pKa = 7.26 KK2 pKa = 10.48 VGMRR6 pKa = 11.84 TPSLKK11 pKa = 10.45 KK12 pKa = 9.9 SFKK15 pKa = 10.73 ARR17 pKa = 11.84 TTGKK21 pKa = 10.08 VKK23 pKa = 10.58 KK24 pKa = 9.91 QMKK27 pKa = 10.05 KK28 pKa = 10.3 SVNPLYY34 pKa = 10.56 GKK36 pKa = 10.42 KK37 pKa = 10.82 GMGLVDD43 pKa = 3.57 NPKK46 pKa = 9.44 KK47 pKa = 10.39 AVYY50 pKa = 10.18 NKK52 pKa = 9.62 VYY54 pKa = 11.05 NKK56 pKa = 7.92 TTVDD60 pKa = 3.14 GLKK63 pKa = 9.65 PLKK66 pKa = 10.54 VSSSKK71 pKa = 9.28 KK72 pKa = 7.7 TNKK75 pKa = 10.22 ANDD78 pKa = 3.26 NFGQVLPLFEE88 pKa = 5.08 PKK90 pKa = 9.04 VTEE93 pKa = 5.21 DD94 pKa = 3.95 IIVHH98 pKa = 6.0 KK99 pKa = 10.57 KK100 pKa = 10.25 KK101 pKa = 9.96 FWTIVSYY108 pKa = 9.56 FFAGLFCCC116 pKa = 5.85
Molecular weight: 13.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.093
IPC2_protein 9.604
IPC_protein 9.458
Toseland 10.555
ProMoST 9.999
Dawson 10.628
Bjellqvist 10.189
Wikipedia 10.716
Rodwell 11.506
Grimsley 10.657
Solomon 10.643
Lehninger 10.628
Nozaki 10.511
DTASelect 10.189
Thurlkill 10.526
EMBOSS 10.906
Sillero 10.54
Patrickios 11.228
IPC_peptide 10.657
IPC2_peptide 8.639
IPC2.peptide.svr19 8.531
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
19
0
19
2944
48
520
154.9
17.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.857 ± 0.303
0.781 ± 0.169
6.25 ± 0.637
8.492 ± 0.918
4.823 ± 0.458
4.552 ± 0.449
1.121 ± 0.154
8.152 ± 0.591
10.19 ± 0.884
10.836 ± 0.661
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.48 ± 0.174
6.42 ± 0.457
2.072 ± 0.261
4.11 ± 0.342
3.499 ± 0.492
5.605 ± 0.494
5.129 ± 0.42
5.639 ± 0.439
0.747 ± 0.128
4.246 ± 0.205
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here