Salinimonas sp. KX18D6
Average proteome isoelectric point is 6.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3632 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5B7Y9F5|A0A5B7Y9F5_9ALTE AbgT family transporter OS=Salinimonas sp. KX18D6 OX=2572577 GN=FBQ74_01685 PE=4 SV=1
MM1 pKa = 7.59 KK2 pKa = 10.32 LAILSSMFALSVVTSGLAAAYY23 pKa = 7.22 EE24 pKa = 4.0 QGDD27 pKa = 3.58 IVIRR31 pKa = 11.84 GGATTVAPDD40 pKa = 3.63 EE41 pKa = 4.39 STSTIMAGGTDD52 pKa = 3.88 LGVDD56 pKa = 3.72 LTIDD60 pKa = 3.56 SNTQLGLNIAYY71 pKa = 9.92 FLTDD75 pKa = 4.71 RR76 pKa = 11.84 INLEE80 pKa = 4.31 LLVATPFKK88 pKa = 10.67 HH89 pKa = 7.42 DD90 pKa = 3.18 VDD92 pKa = 4.74 FGVADD97 pKa = 3.93 PLGTGNQLGEE107 pKa = 4.42 VTHH110 pKa = 6.84 LPPTLTVNYY119 pKa = 9.11 YY120 pKa = 10.35 FNDD123 pKa = 3.75 SASVLQPYY131 pKa = 9.56 VGAGVNYY138 pKa = 8.71 TFIYY142 pKa = 10.72 DD143 pKa = 3.95 EE144 pKa = 4.79 EE145 pKa = 4.31 FTGANRR151 pKa = 11.84 EE152 pKa = 4.18 AGLSDD157 pKa = 4.6 LSLDD161 pKa = 3.61 NSVGLSAQIGLDD173 pKa = 4.13 VMLADD178 pKa = 3.36 NWHH181 pKa = 6.79 INTSVRR187 pKa = 11.84 WIDD190 pKa = 2.99 IDD192 pKa = 3.78 TEE194 pKa = 4.13 ASFKK198 pKa = 10.97 VGEE201 pKa = 4.16 ASGQVSSIEE210 pKa = 3.77 IDD212 pKa = 2.98 PWVYY216 pKa = 9.84 TLSLGYY222 pKa = 9.02 TFF224 pKa = 5.75
Molecular weight: 23.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.699
IPC2_protein 3.795
IPC_protein 3.783
Toseland 3.567
ProMoST 3.961
Dawson 3.783
Bjellqvist 3.948
Wikipedia 3.732
Rodwell 3.617
Grimsley 3.478
Solomon 3.77
Lehninger 3.732
Nozaki 3.91
DTASelect 4.151
Thurlkill 3.63
EMBOSS 3.745
Sillero 3.91
Patrickios 1.036
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.81
Protein with the highest isoelectric point:
>tr|A0A5B7YIK3|A0A5B7YIK3_9ALTE Translational regulator CsrA OS=Salinimonas sp. KX18D6 OX=2572577 GN=csrA PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLKK11 pKa = 10.14 RR12 pKa = 11.84 KK13 pKa = 9.13 RR14 pKa = 11.84 SHH16 pKa = 6.17 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 NGRR28 pKa = 11.84 KK29 pKa = 9.16 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.29 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LSAA44 pKa = 3.91
Molecular weight: 5.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.676
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.398
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3632
0
3632
1210490
37
4582
333.3
37.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.12 ± 0.044
0.973 ± 0.014
5.963 ± 0.039
6.181 ± 0.041
4.108 ± 0.028
6.738 ± 0.043
2.283 ± 0.022
5.907 ± 0.03
4.9 ± 0.035
9.998 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.581 ± 0.02
4.085 ± 0.026
4.023 ± 0.02
4.568 ± 0.029
4.974 ± 0.032
6.683 ± 0.029
5.564 ± 0.025
7.012 ± 0.03
1.262 ± 0.016
3.077 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here