Culicoidibacter larvae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Culicoidibacteria; Culicoidibacterales; Culicoidibacteraceae; Culicoidibacter

Average proteome isoelectric point is 5.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2363 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5R8Q859|A0A5R8Q859_9FIRM Histidine kinase OS=Culicoidibacter larvae OX=2579976 GN=FEZ08_10190 PE=4 SV=1
MM1 pKa = 7.31KK2 pKa = 10.32LVRR5 pKa = 11.84VTLLAIAASLLLAACGKK22 pKa = 9.76PIIEE26 pKa = 5.07RR27 pKa = 11.84DD28 pKa = 3.35AQYY31 pKa = 11.61QMGDD35 pKa = 3.42FAVTEE40 pKa = 3.97NAEE43 pKa = 4.31FGVSATEE50 pKa = 4.22VILEE54 pKa = 4.29EE55 pKa = 5.68GDD57 pKa = 4.14DD58 pKa = 3.9GVEE61 pKa = 3.98QVVVDD66 pKa = 3.94IMIVNNSLDD75 pKa = 3.34IFNVSDD81 pKa = 4.94LDD83 pKa = 3.36IDD85 pKa = 4.47YY86 pKa = 7.22YY87 pKa = 11.0TPTGEE92 pKa = 4.07EE93 pKa = 3.98LEE95 pKa = 4.45YY96 pKa = 10.92TSVKK100 pKa = 10.85DD101 pKa = 3.36PGEE104 pKa = 3.99NYY106 pKa = 8.76MLPGGNTLATLFLKK120 pKa = 9.53YY121 pKa = 9.31TEE123 pKa = 5.12DD124 pKa = 2.95GTYY127 pKa = 10.82YY128 pKa = 11.17LNFLDD133 pKa = 4.36ATDD136 pKa = 4.75KK137 pKa = 8.87EE138 pKa = 4.5TLYY141 pKa = 11.08AIEE144 pKa = 4.21VKK146 pKa = 10.35KK147 pKa = 11.35GEE149 pKa = 4.49VIEE152 pKa = 4.32KK153 pKa = 10.4ALPEE157 pKa = 4.19VTNTASADD165 pKa = 3.37LGSNVEE171 pKa = 4.26KK172 pKa = 10.87GVASGLGNLEE182 pKa = 4.03FTFGTTNIYY191 pKa = 9.12TLTSFGSDD199 pKa = 2.74QLYY202 pKa = 10.69YY203 pKa = 10.78GVEE206 pKa = 3.7LTIRR210 pKa = 11.84NTGSDD215 pKa = 3.06AVNFDD220 pKa = 4.05GYY222 pKa = 11.14SFYY225 pKa = 10.87QIYY228 pKa = 8.95TYY230 pKa = 11.02QDD232 pKa = 3.15NTVSFSSVMNQIGSDD247 pKa = 3.7DD248 pKa = 4.69DD249 pKa = 4.22DD250 pKa = 3.86VLEE253 pKa = 4.8IGEE256 pKa = 4.48VTTLEE261 pKa = 4.26AGEE264 pKa = 4.5SKK266 pKa = 10.78AVTLMVSSSPSAGKK280 pKa = 10.08DD281 pKa = 2.85LFARR285 pKa = 11.84YY286 pKa = 9.47IEE288 pKa = 4.01ITFDD292 pKa = 3.65GEE294 pKa = 4.05KK295 pKa = 8.92ITYY298 pKa = 9.8KK299 pKa = 10.69FF300 pKa = 3.42

Molecular weight: 32.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5R8QHH0|A0A5R8QHH0_9FIRM Uncharacterized protein OS=Culicoidibacter larvae OX=2579976 GN=FEZ08_01105 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.59RR3 pKa = 11.84TFQPNKK9 pKa = 8.79RR10 pKa = 11.84KK11 pKa = 9.75RR12 pKa = 11.84SKK14 pKa = 10.38KK15 pKa = 8.31HH16 pKa = 4.52GFRR19 pKa = 11.84ARR21 pKa = 11.84ISTPTGRR28 pKa = 11.84NILKK32 pKa = 10.13RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.41GRR39 pKa = 11.84KK40 pKa = 8.78VLSAA44 pKa = 4.05

Molecular weight: 5.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2363

0

2363

740341

26

4917

313.3

34.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.046 ± 0.062

0.729 ± 0.016

5.803 ± 0.042

6.513 ± 0.057

4.502 ± 0.046

6.616 ± 0.058

1.754 ± 0.028

8.1 ± 0.056

5.756 ± 0.044

9.18 ± 0.072

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.621 ± 0.03

5.096 ± 0.054

3.301 ± 0.032

3.897 ± 0.043

3.723 ± 0.047

5.924 ± 0.044

6.113 ± 0.1

7.351 ± 0.058

0.921 ± 0.017

4.055 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski