Dictyobacter vulcani
Average proteome isoelectric point is 6.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6350 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5J4KP24|A0A5J4KP24_9CHLR Uncharacterized protein OS=Dictyobacter vulcani OX=2607529 GN=KDW_56500 PE=4 SV=1
MM1 pKa = 7.41 TSSPQQSQPAATSSDD16 pKa = 3.5 QPLSDD21 pKa = 4.23 SLPVSAACSPDD32 pKa = 3.47 AAEE35 pKa = 4.7 HH36 pKa = 6.64 CLTCGDD42 pKa = 3.62 VAVSVRR48 pKa = 11.84 VVQVNVEE55 pKa = 3.88 QGTALVTVEE64 pKa = 4.37 STTEE68 pKa = 4.0 EE69 pKa = 3.85 IDD71 pKa = 3.26 ISLLDD76 pKa = 3.77 SVAVGDD82 pKa = 3.97 TVLVHH87 pKa = 6.53 GGVALEE93 pKa = 3.96 RR94 pKa = 11.84 CGLSMHH100 pKa = 6.32 GEE102 pKa = 4.17 EE103 pKa = 5.38 VDD105 pKa = 3.99 VDD107 pKa = 4.01 DD108 pKa = 5.46
Molecular weight: 11.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.739
IPC2_protein 3.732
IPC_protein 3.668
Toseland 3.478
ProMoST 3.783
Dawson 3.668
Bjellqvist 3.884
Wikipedia 3.617
Rodwell 3.516
Grimsley 3.389
Solomon 3.643
Lehninger 3.605
Nozaki 3.795
DTASelect 3.999
Thurlkill 3.541
EMBOSS 3.63
Sillero 3.795
Patrickios 0.769
IPC_peptide 3.643
IPC2_peptide 3.77
IPC2.peptide.svr19 3.764
Protein with the highest isoelectric point:
>tr|A0A5J4KFV2|A0A5J4KFV2_9CHLR Uncharacterized protein OS=Dictyobacter vulcani OX=2607529 GN=KDW_04270 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.45 RR3 pKa = 11.84 TWQPKK8 pKa = 8.26 RR9 pKa = 11.84 IPRR12 pKa = 11.84 KK13 pKa = 9.21 RR14 pKa = 11.84 EE15 pKa = 3.41 HH16 pKa = 7.15 GFMKK20 pKa = 10.47 RR21 pKa = 11.84 MSTRR25 pKa = 11.84 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VLKK32 pKa = 10.49 ARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.41 GRR39 pKa = 11.84 WSLTVV44 pKa = 3.02
Molecular weight: 5.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.425
IPC2_protein 10.906
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.369
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.106
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.087
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6350
0
6350
1951268
39
8292
307.3
34.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.197 ± 0.031
0.988 ± 0.01
4.871 ± 0.02
5.811 ± 0.036
3.587 ± 0.02
6.826 ± 0.028
2.635 ± 0.019
6.045 ± 0.024
3.594 ± 0.028
10.881 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.352 ± 0.015
3.426 ± 0.024
5.055 ± 0.022
5.409 ± 0.033
5.845 ± 0.029
6.139 ± 0.029
6.063 ± 0.033
6.69 ± 0.027
1.441 ± 0.013
3.146 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here