Dysgonomonas gadei ATCC BAA-286
Average proteome isoelectric point is 6.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4153 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F5IZZ3|F5IZZ3_9BACT Uncharacterized protein OS=Dysgonomonas gadei ATCC BAA-286 OX=742766 GN=HMPREF9455_02660 PE=3 SV=1
MM1 pKa = 7.55 SNEE4 pKa = 4.21 KK5 pKa = 10.71 DD6 pKa = 3.5 LIQYY10 pKa = 10.66 DD11 pKa = 3.52 EE12 pKa = 4.93 DD13 pKa = 4.26 DD14 pKa = 3.52 AVKK17 pKa = 10.31 FIQNYY22 pKa = 9.4 LPQEE26 pKa = 4.17 MKK28 pKa = 10.74 GKK30 pKa = 8.27 YY31 pKa = 8.83 TNDD34 pKa = 3.51 EE35 pKa = 3.67 INYY38 pKa = 9.76 IIDD41 pKa = 3.43 IVYY44 pKa = 10.59 DD45 pKa = 4.5 FYY47 pKa = 11.76 DD48 pKa = 3.41 EE49 pKa = 4.32 KK50 pKa = 11.58 GFMNDD55 pKa = 2.92 EE56 pKa = 4.07 TDD58 pKa = 3.42 EE59 pKa = 4.31 EE60 pKa = 5.04 SVVDD64 pKa = 3.6 IDD66 pKa = 3.52 EE67 pKa = 4.91 DD68 pKa = 4.4 EE69 pKa = 4.12 IVAYY73 pKa = 8.88 VLKK76 pKa = 9.44 YY77 pKa = 8.61 TKK79 pKa = 9.89 KK80 pKa = 10.52 DD81 pKa = 3.07 KK82 pKa = 11.33 VNNFSEE88 pKa = 4.69 DD89 pKa = 3.29 EE90 pKa = 3.94 IAFIIQGEE98 pKa = 4.22 LAYY101 pKa = 10.17 CDD103 pKa = 3.56 SIGIFEE109 pKa = 4.29
Molecular weight: 12.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.742
IPC2_protein 3.745
IPC_protein 3.732
Toseland 3.516
ProMoST 3.872
Dawson 3.719
Bjellqvist 3.91
Wikipedia 3.643
Rodwell 3.554
Grimsley 3.427
Solomon 3.706
Lehninger 3.656
Nozaki 3.834
DTASelect 4.037
Thurlkill 3.579
EMBOSS 3.656
Sillero 3.846
Patrickios 1.85
IPC_peptide 3.706
IPC2_peptide 3.821
IPC2.peptide.svr19 3.779
Protein with the highest isoelectric point:
>tr|F5J3P4|F5J3P4_9BACT Uncharacterized protein OS=Dysgonomonas gadei ATCC BAA-286 OX=742766 GN=HMPREF9455_03961 PE=4 SV=1
MM1 pKa = 7.23 YY2 pKa = 10.68 SDD4 pKa = 4.0 YY5 pKa = 11.62 LNLKK9 pKa = 10.05 LVTLSHH15 pKa = 6.38 YY16 pKa = 10.53 CRR18 pKa = 11.84 ALPSLPSLLALPKK31 pKa = 10.33 SKK33 pKa = 10.68 QKK35 pKa = 11.15 ASAKK39 pKa = 10.25 FLGDD43 pKa = 3.93 LSTACRR49 pKa = 11.84 LKK51 pKa = 9.71 VTNGLSPRR59 pKa = 11.84 PFLRR63 pKa = 11.84 RR64 pKa = 11.84 LHH66 pKa = 5.04 WTGARR71 pKa = 11.84 LRR73 pKa = 11.84 NQCASADD80 pKa = 3.93 AIVRR84 pKa = 11.84 QSPFEE89 pKa = 3.99 NKK91 pKa = 8.62 ICVSQRR97 pKa = 11.84 HH98 pKa = 5.0 TLHH101 pKa = 6.07 GRR103 pKa = 11.84 APVRR107 pKa = 11.84 RR108 pKa = 11.84 EE109 pKa = 3.31 RR110 pKa = 11.84 LKK112 pKa = 11.01 NEE114 pKa = 3.88 NVFEE118 pKa = 4.94 LII120 pKa = 4.32
Molecular weight: 13.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.346
IPC2_protein 9.706
IPC_protein 10.452
Toseland 10.774
ProMoST 10.438
Dawson 10.862
Bjellqvist 10.57
Wikipedia 11.052
Rodwell 11.125
Grimsley 10.906
Solomon 10.979
Lehninger 10.95
Nozaki 10.774
DTASelect 10.555
Thurlkill 10.774
EMBOSS 11.184
Sillero 10.804
Patrickios 10.862
IPC_peptide 10.994
IPC2_peptide 9.736
IPC2.peptide.svr19 8.519
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4153
0
4153
1514011
29
2478
364.6
41.2
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.458 ± 0.036
0.949 ± 0.012
5.82 ± 0.027
6.199 ± 0.038
4.775 ± 0.026
6.704 ± 0.036
1.642 ± 0.014
7.841 ± 0.043
7.298 ± 0.034
8.811 ± 0.035
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.5 ± 0.016
5.755 ± 0.033
3.592 ± 0.02
3.235 ± 0.02
4.035 ± 0.021
6.613 ± 0.027
5.663 ± 0.031
6.14 ± 0.026
1.267 ± 0.016
4.703 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here