Arthrobacter rhombi
Average proteome isoelectric point is 6.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3075 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1R4F9Q2|A0A1R4F9Q2_9MICC GST C-terminal domain-containing protein OS=Arthrobacter rhombi OX=71253 GN=FM101_02875 PE=4 SV=1
MM1 pKa = 7.35 SKK3 pKa = 10.19 VLSTLRR9 pKa = 11.84 YY10 pKa = 8.08 SAEE13 pKa = 4.2 HH14 pKa = 6.21 EE15 pKa = 4.36 WVDD18 pKa = 3.4 DD19 pKa = 3.62 STPIRR24 pKa = 11.84 VGITQIAADD33 pKa = 3.69 ALGDD37 pKa = 3.73 VVYY40 pKa = 11.27 VDD42 pKa = 4.28 LPEE45 pKa = 4.4 VGSTVTAGEE54 pKa = 4.18 TCGEE58 pKa = 4.21 VEE60 pKa = 4.36 STKK63 pKa = 10.75 SVSDD67 pKa = 3.74 LYY69 pKa = 11.56 APVTGTIVEE78 pKa = 4.39 TNPAVEE84 pKa = 5.03 DD85 pKa = 3.79 NPALLNEE92 pKa = 4.46 DD93 pKa = 4.12 PYY95 pKa = 11.68 GGGWLFTVEE104 pKa = 4.16 VSEE107 pKa = 4.5 EE108 pKa = 4.41 GPLLSAADD116 pKa = 3.76 YY117 pKa = 11.24 ASANGGDD124 pKa = 3.79 VEE126 pKa = 4.41
Molecular weight: 13.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.774
IPC2_protein 3.656
IPC_protein 3.592
Toseland 3.401
ProMoST 3.732
Dawson 3.579
Bjellqvist 3.795
Wikipedia 3.503
Rodwell 3.427
Grimsley 3.325
Solomon 3.554
Lehninger 3.503
Nozaki 3.706
DTASelect 3.859
Thurlkill 3.465
EMBOSS 3.516
Sillero 3.719
Patrickios 0.769
IPC_peptide 3.554
IPC2_peptide 3.694
IPC2.peptide.svr19 3.703
Protein with the highest isoelectric point:
>tr|A0A1R4EY14|A0A1R4EY14_9MICC ABC-type nitrate/sulfonate/bicarbonate transport system permease component OS=Arthrobacter rhombi OX=71253 GN=FM101_01315 PE=3 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 9.97 KK16 pKa = 9.33 HH17 pKa = 4.25 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILSARR35 pKa = 11.84 RR36 pKa = 11.84 SKK38 pKa = 11.01 GRR40 pKa = 11.84 TEE42 pKa = 4.38 LSAA45 pKa = 4.86
Molecular weight: 5.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.44
IPC2_protein 10.95
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.34
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.076
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.112
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3075
0
3075
982341
37
2029
319.5
34.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.535 ± 0.063
0.605 ± 0.012
5.738 ± 0.036
5.972 ± 0.048
3.174 ± 0.026
9.156 ± 0.034
2.176 ± 0.019
4.577 ± 0.034
2.58 ± 0.041
10.3 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.112 ± 0.018
2.321 ± 0.022
5.274 ± 0.031
3.217 ± 0.02
6.631 ± 0.047
5.979 ± 0.03
6.05 ± 0.029
8.169 ± 0.036
1.412 ± 0.018
2.021 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here