Onchocerca flexuosa

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Spirurina; Spiruromorpha; Filarioidea; Onchocercidae; Onchocerca

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8122 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A183HED8|A0A183HED8_9BILA ZP domain-containing protein OS=Onchocerca flexuosa OX=387005 GN=OFLC_LOCUS5851 PE=4 SV=1
MM1 pKa = 7.85PNFDD5 pKa = 5.03KK6 pKa = 10.8YY7 pKa = 9.49TATDD11 pKa = 3.48APLVLIIQLMIPLIIWQFIIGISFFPDD38 pKa = 2.92EE39 pKa = 5.36FIAQISDD46 pKa = 3.39DD47 pKa = 4.67FYY49 pKa = 11.27GHH51 pKa = 6.46NNAAGDD57 pKa = 3.54QSFYY61 pKa = 11.21QSFPNLVDD69 pKa = 4.4TINSHH74 pKa = 6.03GDD76 pKa = 3.15VPRR79 pKa = 11.84SVISDD84 pKa = 3.66EE85 pKa = 4.25QISPYY90 pKa = 10.75FMLL93 pKa = 5.47

Molecular weight: 10.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A238BK30|A0A238BK30_9BILA Uncharacterized protein OS=Onchocerca flexuosa OX=387005 GN=X798_08100 PE=4 SV=1
MM1 pKa = 7.86PAPKK5 pKa = 10.07GRR7 pKa = 11.84PKK9 pKa = 10.45ARR11 pKa = 11.84AIRR14 pKa = 11.84RR15 pKa = 11.84VPVVRR20 pKa = 11.84RR21 pKa = 11.84NVQPVIRR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84SPSRR34 pKa = 11.84SSSSSRR40 pKa = 11.84SRR42 pKa = 11.84SFTTKK47 pKa = 9.2TSSSRR52 pKa = 11.84SSRR55 pKa = 11.84SHH57 pKa = 4.21SRR59 pKa = 11.84KK60 pKa = 8.57YY61 pKa = 10.2KK62 pKa = 8.18RR63 pKa = 11.84SHH65 pKa = 6.27RR66 pKa = 11.84RR67 pKa = 11.84RR68 pKa = 11.84ISHH71 pKa = 6.64RR72 pKa = 11.84LRR74 pKa = 11.84RR75 pKa = 11.84RR76 pKa = 11.84SNSVIPLYY84 pKa = 9.56GTSGTVIVGYY94 pKa = 10.62VRR96 pKa = 11.84LCRR99 pKa = 11.84SGVKK103 pKa = 9.74LCHH106 pKa = 6.76RR107 pKa = 11.84NGG109 pKa = 3.27

Molecular weight: 12.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8122

0

8122

3352156

15

6827

412.7

46.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.114 ± 0.025

2.186 ± 0.023

5.39 ± 0.022

6.607 ± 0.034

4.339 ± 0.019

4.947 ± 0.029

2.415 ± 0.011

6.747 ± 0.024

6.26 ± 0.03

9.313 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.531 ± 0.012

5.2 ± 0.019

4.277 ± 0.027

4.126 ± 0.022

5.674 ± 0.02

8.273 ± 0.027

5.489 ± 0.019

5.821 ± 0.02

1.097 ± 0.008

3.191 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski