Leifsonia sp. Root112D2

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Leifsonia; unclassified Leifsonia

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3046 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q6PZR2|A0A0Q6PZR2_9MICO Uncharacterized protein OS=Leifsonia sp. Root112D2 OX=1736426 GN=ASC63_05435 PE=4 SV=1
MM1 pKa = 6.9VAILASLLVLASSLAPTLAAEE22 pKa = 4.42AAGPAVVAVTVSITDD37 pKa = 3.27PTGAVVSEE45 pKa = 4.23VDD47 pKa = 3.3ASSMANYY54 pKa = 9.4SVNVAYY60 pKa = 10.3SCSVADD66 pKa = 3.67CTGVQVVIDD75 pKa = 4.25PTVLDD80 pKa = 5.0PIYY83 pKa = 10.36GQYY86 pKa = 10.37RR87 pKa = 11.84KK88 pKa = 8.21EE89 pKa = 4.06TAVTFTPPFSPAPSFTGSLGAGYY112 pKa = 7.94TVDD115 pKa = 3.95LGTVAVGTGGSFKK128 pKa = 10.55LAYY131 pKa = 10.13RR132 pKa = 11.84IDD134 pKa = 3.42ARR136 pKa = 11.84YY137 pKa = 10.18SGPQPGSFFLDD148 pKa = 4.21GSPITARR155 pKa = 11.84ATVSASNADD164 pKa = 3.44SSAVGTASANWVSSIPAAPTARR186 pKa = 11.84LSAPASIRR194 pKa = 11.84TDD196 pKa = 3.31TPVTITAEE204 pKa = 4.17GDD206 pKa = 3.94SRR208 pKa = 11.84CLSYY212 pKa = 11.31NGAGVLLGIPWLTCAKK228 pKa = 10.41DD229 pKa = 3.36YY230 pKa = 7.63TAPVTLPADD239 pKa = 3.68AQYY242 pKa = 11.4VAGSGGSYY250 pKa = 10.36NAGTRR255 pKa = 11.84TVTFSQTGAAAAGGLAFNDD274 pKa = 3.16NTFQIVFPSDD284 pKa = 3.26AFPTSGDD291 pKa = 3.24GCVASEE297 pKa = 4.21VFSVTEE303 pKa = 3.73TMTYY307 pKa = 10.89LDD309 pKa = 3.8GTVRR313 pKa = 11.84TADD316 pKa = 3.72PASTAIKK323 pKa = 9.98VQNCTPFAKK332 pKa = 10.44GALSKK337 pKa = 10.96AIGGRR342 pKa = 11.84DD343 pKa = 3.1GGTPTDD349 pKa = 4.81AIVDD353 pKa = 3.7IPLTPGGVNNKK364 pKa = 7.76YY365 pKa = 9.1WQVEE369 pKa = 3.78ADD371 pKa = 3.46NQANVAGVATITDD384 pKa = 3.79ADD386 pKa = 4.38LGQDD390 pKa = 3.97DD391 pKa = 4.72MPITSIRR398 pKa = 11.84IVGGGPATVNYY409 pKa = 9.16TLDD412 pKa = 3.86DD413 pKa = 3.98ATTGSATVAQGATWTPPSGHH433 pKa = 6.67TIAGVSATSSLLVGPNSVEE452 pKa = 3.86SGTAVTRR459 pKa = 11.84FIIRR463 pKa = 11.84LNFSVHH469 pKa = 7.29DD470 pKa = 3.99GTTPGPRR477 pKa = 11.84TNTATASIAYY487 pKa = 8.26PDD489 pKa = 3.79QPEE492 pKa = 5.21LGTLDD497 pKa = 4.31LGSTTATATLQVNADD512 pKa = 3.42VTLNAGGPSVTATGTPIVGGTVGWKK537 pKa = 9.3MSGAMDD543 pKa = 3.98NVAAGANYY551 pKa = 9.21RR552 pKa = 11.84PQYY555 pKa = 11.05VFLAPKK561 pKa = 9.62DD562 pKa = 3.65WNITAGSAAVSGVPGMTYY580 pKa = 10.22DD581 pKa = 3.86YY582 pKa = 10.27KK583 pKa = 10.3TVTYY587 pKa = 10.63NGEE590 pKa = 3.93SYY592 pKa = 11.18DD593 pKa = 3.79AVLATWPAAVTDD605 pKa = 3.65VGKK608 pKa = 10.87FNLPTMTVDD617 pKa = 3.92TVPTGAATAGTNNQTAYY634 pKa = 11.2LFLGDD639 pKa = 3.99VNNGPVALYY648 pKa = 10.24YY649 pKa = 10.43PNAYY653 pKa = 8.74TDD655 pKa = 4.32ASDD658 pKa = 4.47FDD660 pKa = 4.88GDD662 pKa = 3.99GNATEE667 pKa = 4.26QYY669 pKa = 9.52SWRR672 pKa = 11.84SNTTSLAPSKK682 pKa = 10.7SIAVTKK688 pKa = 8.4QICHH692 pKa = 7.05PDD694 pKa = 3.16AEE696 pKa = 4.49QADD699 pKa = 4.27GCDD702 pKa = 3.67WISDD706 pKa = 3.82SNITVGVPPNATAIKK721 pKa = 10.45YY722 pKa = 9.62RR723 pKa = 11.84VTLANSGNVDD733 pKa = 4.0LNNVVAYY740 pKa = 10.23DD741 pKa = 3.43VLPYY745 pKa = 10.99KK746 pKa = 10.67NDD748 pKa = 3.35TGLTDD753 pKa = 4.11GTASTPRR760 pKa = 11.84GSTVQEE766 pKa = 3.95VLATVSDD773 pKa = 3.98VSAGVDD779 pKa = 3.46LSYY782 pKa = 11.3SGSTNPARR790 pKa = 11.84PQVYY794 pKa = 9.06TGTTDD799 pKa = 3.46ADD801 pKa = 3.5DD802 pKa = 3.54WGTTVTEE809 pKa = 4.43AKK811 pKa = 10.32AIRR814 pKa = 11.84AQVTSLAAGASVTFDD829 pKa = 3.57YY830 pKa = 10.36EE831 pKa = 3.92ASLVDD836 pKa = 3.51GTANLVACNSIAATAATLAPIEE858 pKa = 4.23PAAVCASTQAADD870 pKa = 4.46LEE872 pKa = 4.67ITAPAHH878 pKa = 7.13LPMQQGRR885 pKa = 11.84PGAADD890 pKa = 3.21FTVINHH896 pKa = 6.72GGSPLSPATVTAVIPAGFSVTDD918 pKa = 4.23LAPIGYY924 pKa = 9.25ACSDD928 pKa = 3.61AAGDD932 pKa = 3.97SAPIAGPVTLSCVTQKK948 pKa = 11.0PDD950 pKa = 2.95GTVRR954 pKa = 11.84NLSLDD959 pKa = 3.77APDD962 pKa = 3.94TLSIPVLVDD971 pKa = 3.29EE972 pKa = 5.4DD973 pKa = 3.95ATGPSCIDD981 pKa = 3.25ASVTGTLYY989 pKa = 11.13DD990 pKa = 4.6PDD992 pKa = 3.92LTNNDD997 pKa = 3.26TSFCAAVADD1006 pKa = 4.11STLPLITLDD1015 pKa = 3.5KK1016 pKa = 10.5TDD1018 pKa = 3.5NTTSVTVGEE1027 pKa = 4.18QTTYY1031 pKa = 10.64SLTVASHH1038 pKa = 6.71LSSEE1042 pKa = 4.28AVAGAVLTDD1051 pKa = 3.55TLPAGEE1057 pKa = 4.59TFVSASDD1064 pKa = 3.43GGTASGQTVTWNLAEE1079 pKa = 4.63LNPTGAPSADD1089 pKa = 3.38GSDD1092 pKa = 3.49TTGGSGAAITVTVTVQIDD1110 pKa = 3.8DD1111 pKa = 4.05GTQDD1115 pKa = 3.5TLFNTASVSAPDD1127 pKa = 3.85PAGGPTLTDD1136 pKa = 3.28GATDD1140 pKa = 3.42TDD1142 pKa = 3.78TVRR1145 pKa = 11.84NVFTDD1150 pKa = 4.22LDD1152 pKa = 4.0PAIATPQNQSINTPLSDD1169 pKa = 3.24IVTTTGASLDD1179 pKa = 4.02PASVAQNTAPTHH1191 pKa = 5.39GSITIDD1197 pKa = 3.31TTTGAVTYY1205 pKa = 9.75IPAPGYY1211 pKa = 10.51SGADD1215 pKa = 3.19SYY1217 pKa = 11.94EE1218 pKa = 3.91VTVCDD1223 pKa = 3.57TSSPAQCHH1231 pKa = 4.9VSTVTVAVGVNVVDD1245 pKa = 4.8AVNDD1249 pKa = 3.8TDD1251 pKa = 3.34ATTAVTPVTTDD1262 pKa = 2.86VTGNDD1267 pKa = 3.77TTQSGQPLAHH1277 pKa = 6.27PTVPTSPTHH1286 pKa = 6.04GSAVVNGDD1294 pKa = 3.1GTITYY1299 pKa = 8.39TPAAAWSGTDD1309 pKa = 2.83SYY1311 pKa = 11.64GYY1313 pKa = 8.25QLCDD1317 pKa = 2.9TSTPTPVCDD1326 pKa = 3.14TATVQVTVANVFLDD1340 pKa = 3.91ATPSIPTAQNAPVTTPLADD1359 pKa = 2.95IVTTTGLLLDD1369 pKa = 4.23PTQVTQEE1376 pKa = 3.97TAPAHH1381 pKa = 5.62GSIAIDD1387 pKa = 3.44PHH1389 pKa = 7.92SGAVTYY1395 pKa = 9.7TPTAGYY1401 pKa = 8.51TGDD1404 pKa = 4.06DD1405 pKa = 3.54SYY1407 pKa = 11.96AIQVCDD1413 pKa = 3.55TDD1415 pKa = 5.23GVCHH1419 pKa = 6.56NSPVSVSVGANVVVVQDD1436 pKa = 3.46ATIGTRR1442 pKa = 11.84VNTDD1446 pKa = 3.12ADD1448 pKa = 3.96PVDD1451 pKa = 4.06VLTTATSASGQPLGNPTITDD1471 pKa = 3.56QPGHH1475 pKa = 5.46GTVVVNADD1483 pKa = 3.27GTISYY1488 pKa = 10.36GPEE1491 pKa = 3.47TDD1493 pKa = 3.83YY1494 pKa = 11.64VGSDD1498 pKa = 2.71SFTYY1502 pKa = 8.08TVCDD1506 pKa = 3.38TGSPTQACDD1515 pKa = 2.7TGTITVTVVAIADD1528 pKa = 3.94LVSTKK1533 pKa = 10.12TLDD1536 pKa = 3.28TATVVPGLPVSYY1548 pKa = 9.0TAMLHH1553 pKa = 6.7NSGPWTATNVHH1564 pKa = 6.67SIDD1567 pKa = 4.96PIDD1570 pKa = 3.97KK1571 pKa = 10.16RR1572 pKa = 11.84VMHH1575 pKa = 7.01AKK1577 pKa = 8.04GTPDD1581 pKa = 2.99ASVPGGLCLTRR1592 pKa = 11.84PTTTADD1598 pKa = 3.71LARR1601 pKa = 11.84LNPASGPYY1609 pKa = 9.82TIDD1612 pKa = 3.81AYY1614 pKa = 10.08PQVVDD1619 pKa = 4.4CSYY1622 pKa = 11.18PSIPSGTTVHH1632 pKa = 6.47DD1633 pKa = 4.79TITGTVDD1640 pKa = 3.29PAALPGGTILNQAPVYY1656 pKa = 10.45ADD1658 pKa = 4.12SYY1660 pKa = 12.08DD1661 pKa = 3.8PDD1663 pKa = 3.51LSNNVGTATATITAPADD1680 pKa = 3.42VDD1682 pKa = 3.91PTTSAPSGLARR1693 pKa = 11.84TGVDD1697 pKa = 3.26AAPTGILALLLMLAGAGTLFLARR1720 pKa = 11.84RR1721 pKa = 11.84RR1722 pKa = 11.84RR1723 pKa = 11.84RR1724 pKa = 11.84AA1725 pKa = 2.97

Molecular weight: 173.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q6Q0S2|A0A0Q6Q0S2_9MICO Beta-glucosidase OS=Leifsonia sp. Root112D2 OX=1736426 GN=ASC63_01865 PE=3 SV=1
MM1 pKa = 7.33KK2 pKa = 10.29RR3 pKa = 11.84IWPLLVPGVILSAVGLVWTLQGLNVLRR30 pKa = 11.84GSVMSGSSLWATMGPIVLLLGLVLIAIAIARR61 pKa = 11.84RR62 pKa = 11.84RR63 pKa = 11.84RR64 pKa = 11.84KK65 pKa = 9.58RR66 pKa = 3.2

Molecular weight: 7.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3046

0

3046

1001125

37

1981

328.7

35.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.05 ± 0.063

0.484 ± 0.01

5.723 ± 0.035

5.381 ± 0.048

3.232 ± 0.031

8.747 ± 0.036

2.024 ± 0.021

4.913 ± 0.032

2.445 ± 0.035

10.223 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.874 ± 0.018

2.411 ± 0.023

5.089 ± 0.033

2.981 ± 0.022

6.784 ± 0.046

6.172 ± 0.032

6.192 ± 0.043

8.729 ± 0.036

1.445 ± 0.016

2.102 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski