Streptomyces diacarni

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6222 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A367ERD3|A0A367ERD3_9ACTN Adenylate/guanylate cyclase domain-containing protein OS=Streptomyces diacarni OX=2800381 GN=DTL70_19635 PE=3 SV=1
MM1 pKa = 7.48ARR3 pKa = 11.84DD4 pKa = 3.59PDD6 pKa = 3.68FCLVVPEE13 pKa = 5.36DD14 pKa = 4.99FDD16 pKa = 4.44DD17 pKa = 4.24TDD19 pKa = 5.07DD20 pKa = 3.7EE21 pKa = 4.79TGVYY25 pKa = 9.87PIAAKK30 pKa = 10.64LFIASTAAEE39 pKa = 4.6VFRR42 pKa = 11.84KK43 pKa = 8.63AHH45 pKa = 5.24EE46 pKa = 4.24WVGEE50 pKa = 3.77HH51 pKa = 7.72DD52 pKa = 3.52IRR54 pKa = 11.84LVDD57 pKa = 3.77VAWNCLCGEE66 pKa = 4.35EE67 pKa = 4.02EE68 pKa = 4.68TYY70 pKa = 11.39ALSIYY75 pKa = 10.27FAFEE79 pKa = 4.6LDD81 pKa = 3.88PEE83 pKa = 4.48DD84 pKa = 3.67AA85 pKa = 5.21

Molecular weight: 9.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A367E9E1|A0A367E9E1_9ACTN Dihydrodipicolinate synthase family protein OS=Streptomyces diacarni OX=2800381 GN=DTL70_31120 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIVANRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.81GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6222

0

6222

2120821

29

8290

340.9

36.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.011 ± 0.05

0.825 ± 0.01

5.79 ± 0.025

5.988 ± 0.029

2.641 ± 0.016

9.886 ± 0.033

2.439 ± 0.015

2.675 ± 0.022

2.036 ± 0.029

10.332 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.723 ± 0.013

1.584 ± 0.013

6.257 ± 0.032

2.782 ± 0.02

8.336 ± 0.036

5.104 ± 0.021

5.968 ± 0.025

8.232 ± 0.029

1.446 ± 0.012

1.945 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski