Psychrilyobacter sp. S5
Average proteome isoelectric point is 6.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3023 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A369XYD2|A0A369XYD2_9FUSO 30S ribosomal protein S15 OS=Psychrilyobacter sp. S5 OX=2283384 GN=rpsO PE=3 SV=1
MM1 pKa = 7.47 EE2 pKa = 5.07 KK3 pKa = 9.73 WLCVPCGYY11 pKa = 10.22 IYY13 pKa = 10.7 DD14 pKa = 4.23 PEE16 pKa = 6.0 IGDD19 pKa = 3.84 DD20 pKa = 4.07 AGDD23 pKa = 3.6 IEE25 pKa = 5.01 PGVKK29 pKa = 10.16 FEE31 pKa = 5.79 DD32 pKa = 4.48 LPEE35 pKa = 4.87 DD36 pKa = 3.69 WTCPLCGAPKK46 pKa = 10.24 EE47 pKa = 4.32 DD48 pKa = 3.97 FEE50 pKa = 5.01 KK51 pKa = 11.37 VEE53 pKa = 4.06
Molecular weight: 5.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.76
IPC2_protein 3.795
IPC_protein 3.706
Toseland 3.516
ProMoST 3.834
Dawson 3.694
Bjellqvist 3.935
Wikipedia 3.617
Rodwell 3.541
Grimsley 3.439
Solomon 3.656
Lehninger 3.617
Nozaki 3.821
DTASelect 3.973
Thurlkill 3.579
EMBOSS 3.63
Sillero 3.821
Patrickios 0.693
IPC_peptide 3.656
IPC2_peptide 3.795
IPC2.peptide.svr19 3.794
Protein with the highest isoelectric point:
>tr|A0A369XYN0|A0A369XYN0_9FUSO Uncharacterized protein OS=Psychrilyobacter sp. S5 OX=2283384 GN=DV867_10645 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 8.99 RR4 pKa = 11.84 TFQPNKK10 pKa = 8.87 RR11 pKa = 11.84 KK12 pKa = 9.78 RR13 pKa = 11.84 KK14 pKa = 8.78 KK15 pKa = 8.68 EE16 pKa = 3.34 HH17 pKa = 6.0 GFRR20 pKa = 11.84 KK21 pKa = 9.77 RR22 pKa = 11.84 MKK24 pKa = 8.48 TKK26 pKa = 10.45 AGRR29 pKa = 11.84 SVLKK33 pKa = 10.29 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.71 GRR40 pKa = 11.84 TKK42 pKa = 10.86 LSAA45 pKa = 3.61
Molecular weight: 5.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.408
IPC2_protein 10.891
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.471
Grimsley 12.661
Solomon 13.115
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.193
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.02
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3023
0
3023
935799
33
2471
309.6
34.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.496 ± 0.059
0.873 ± 0.018
5.485 ± 0.04
7.888 ± 0.056
4.611 ± 0.035
7.241 ± 0.051
1.509 ± 0.019
9.546 ± 0.053
9.286 ± 0.06
9.504 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.79 ± 0.025
5.381 ± 0.035
2.811 ± 0.023
2.072 ± 0.019
3.584 ± 0.028
5.898 ± 0.032
5.02 ± 0.033
6.301 ± 0.042
0.703 ± 0.014
4.004 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here