Thiohalophilus thiocyanatoxydans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Thioalkalispiraceae; Thiohalophilus

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2775 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R8J2A3|A0A4R8J2A3_9GAMM LemA protein OS=Thiohalophilus thiocyanatoxydans OX=381308 GN=EDC23_0364 PE=3 SV=1
MM1 pKa = 7.52AGAMAQYY8 pKa = 10.08PDD10 pKa = 3.11ARR12 pKa = 11.84APFGLAGRR20 pKa = 11.84EE21 pKa = 3.89SRR23 pKa = 11.84LADD26 pKa = 3.31TLASLRR32 pKa = 11.84RR33 pKa = 11.84GWRR36 pKa = 11.84KK37 pKa = 5.96TARR40 pKa = 11.84IAGALLVGALLLAPAPSANAYY61 pKa = 8.69PADD64 pKa = 3.36QCAADD69 pKa = 4.57RR70 pKa = 11.84FGSDD74 pKa = 4.45LNCTANDD81 pKa = 3.52VAITNMQVIGDD92 pKa = 4.04MTWCEE97 pKa = 4.09GGTDD101 pKa = 2.96IVVDD105 pKa = 4.38LEE107 pKa = 4.49VTVNFASPDD116 pKa = 3.51RR117 pKa = 11.84NDD119 pKa = 2.92IGVFISNDD127 pKa = 3.19GNDD130 pKa = 3.89PQTTEE135 pKa = 4.19ANGGAASCSVSEE147 pKa = 5.25LPTEE151 pKa = 4.65SPFLDD156 pKa = 4.54LDD158 pKa = 4.06GDD160 pKa = 4.12SCGDD164 pKa = 3.57GNQSISGGTGSGTFYY179 pKa = 8.53MTDD182 pKa = 2.89VTVPCQAMPGSGNDD196 pKa = 3.32LYY198 pKa = 11.85VPFVVSWDD206 pKa = 3.49QDD208 pKa = 3.33KK209 pKa = 11.23QGQNVCSDD217 pKa = 3.19EE218 pKa = 4.31RR219 pKa = 11.84DD220 pKa = 3.43PVPRR224 pKa = 11.84TKK226 pKa = 10.45SKK228 pKa = 11.23CNAPDD233 pKa = 3.27IAQGTIQVGVMPSISKK249 pKa = 10.52SDD251 pKa = 3.96DD252 pKa = 2.94IEE254 pKa = 4.42YY255 pKa = 10.38IKK257 pKa = 11.21SGDD260 pKa = 3.48STTYY264 pKa = 11.12DD265 pKa = 3.23VTITNDD271 pKa = 3.1TGDD274 pKa = 3.83EE275 pKa = 4.03LGGAEE280 pKa = 4.33FSDD283 pKa = 4.04PAVSDD288 pKa = 3.54MSVDD292 pKa = 3.79SLNCTASGGATCPTGYY308 pKa = 9.23TIADD312 pKa = 3.67MQGDD316 pKa = 5.5GITLPDD322 pKa = 3.76MPDD325 pKa = 3.31GGSLTFEE332 pKa = 4.29IEE334 pKa = 3.88ATLTGDD340 pKa = 3.87PNDD343 pKa = 4.37TITNTGYY350 pKa = 8.79VTMGSHH356 pKa = 6.36TNSASDD362 pKa = 3.26TNTIVDD368 pKa = 4.88EE369 pKa = 4.48IGISPTSQNKK379 pKa = 9.12EE380 pKa = 3.51GAAGNTVTYY389 pKa = 9.75EE390 pKa = 3.83YY391 pKa = 10.05TVNNFTPWEE400 pKa = 4.18DD401 pKa = 4.66DD402 pKa = 3.01ISLSAVSSEE411 pKa = 3.53GWNVLLSDD419 pKa = 3.63ATLTVDD425 pKa = 4.52SEE427 pKa = 4.61GSADD431 pKa = 3.45FTLEE435 pKa = 3.78VEE437 pKa = 4.59IPAGASIDD445 pKa = 4.79DD446 pKa = 4.32FDD448 pKa = 4.24TTTITATSGDD458 pKa = 4.76DD459 pKa = 3.52PTMTASATAKK469 pKa = 7.12TTVAEE474 pKa = 4.37LLTLVPDD481 pKa = 3.58NTAAGGPGQTFSYY494 pKa = 10.61EE495 pKa = 3.72HH496 pKa = 6.37TLQNNFSSSQTISLSTAMTGDD517 pKa = 3.62CTDD520 pKa = 3.22WQATIYY526 pKa = 10.02EE527 pKa = 4.4ADD529 pKa = 3.78GEE531 pKa = 4.88TVISSVDD538 pKa = 3.44LDD540 pKa = 4.16GNGDD544 pKa = 3.41NTDD547 pKa = 2.88IVVEE551 pKa = 4.32VTAPNSAAIGDD562 pKa = 4.24SCVVTTTGTTSGVSASAKK580 pKa = 8.94DD581 pKa = 3.26TTTIKK586 pKa = 10.67EE587 pKa = 4.59LGLFWDD593 pKa = 4.02PDD595 pKa = 3.99YY596 pKa = 11.69EE597 pKa = 4.31EE598 pKa = 4.66EE599 pKa = 4.74SYY601 pKa = 10.52IYY603 pKa = 9.87PAGNNVYY610 pKa = 10.28GKK612 pKa = 10.71AFGLTDD618 pKa = 3.6GEE620 pKa = 4.42DD621 pKa = 4.84YY622 pKa = 11.14YY623 pKa = 11.42FEE625 pKa = 4.5WYY627 pKa = 10.07DD628 pKa = 3.38PAGDD632 pKa = 3.94LVRR635 pKa = 11.84TSSTTTVLGTVLPDD649 pKa = 3.47TYY651 pKa = 11.1KK652 pKa = 11.15LPDD655 pKa = 4.39DD656 pKa = 4.22ATLGTWNVKK665 pKa = 7.72VWHH668 pKa = 7.02DD669 pKa = 3.7TTPDD673 pKa = 3.5RR674 pKa = 11.84EE675 pKa = 4.33LHH677 pKa = 5.72QEE679 pKa = 3.43TDD681 pKa = 4.1FYY683 pKa = 11.56VGPDD687 pKa = 3.62HH688 pKa = 7.62IEE690 pKa = 4.03ASHH693 pKa = 7.26SGDD696 pKa = 3.96TEE698 pKa = 4.32TTDD701 pKa = 3.36TNVEE705 pKa = 3.73VDD707 pKa = 3.78LALHH711 pKa = 6.74DD712 pKa = 4.51RR713 pKa = 11.84FNHH716 pKa = 7.49AIPEE720 pKa = 4.25DD721 pKa = 4.08SNGDD725 pKa = 4.35LITANPPTTANPLLVTVTVDD745 pKa = 3.4GSASIVSTSLGNATISGQKK764 pKa = 8.13VTGLLDD770 pKa = 4.04DD771 pKa = 4.23TDD773 pKa = 4.13GTATMTITNSVAEE786 pKa = 4.46TVNVTPEE793 pKa = 3.79SHH795 pKa = 6.64EE796 pKa = 3.93PALYY800 pKa = 10.37GSPLRR805 pKa = 11.84DD806 pKa = 3.09EE807 pKa = 4.52SAAIDD812 pKa = 4.32FKK814 pKa = 11.21RR815 pKa = 11.84PPLDD819 pKa = 3.25HH820 pKa = 7.23FRR822 pKa = 11.84LEE824 pKa = 4.3YY825 pKa = 10.55NGQGLTCQPKK835 pKa = 9.92SITVKK840 pKa = 10.74ACIDD844 pKa = 3.76TNCNNLYY851 pKa = 10.43AYY853 pKa = 9.58DD854 pKa = 3.9VSVSLSPGGWVGGDD868 pKa = 3.2NKK870 pKa = 10.25VLSGGSEE877 pKa = 3.83EE878 pKa = 4.26FEE880 pKa = 4.31LRR882 pKa = 11.84KK883 pKa = 7.46TTTGTVTLDD892 pKa = 3.36IDD894 pKa = 4.14SSSPSPNNTPVCVNTATDD912 pKa = 4.11NEE914 pKa = 4.71SCDD917 pKa = 3.87LTFHH921 pKa = 7.8DD922 pKa = 5.28SGFLVDD928 pKa = 5.41VPTQTSCQTSGDD940 pKa = 3.77LTLSAVRR947 pKa = 11.84TDD949 pKa = 5.65DD950 pKa = 3.54VTQKK954 pKa = 10.34CVPAFASTTKK964 pKa = 10.53NLKK967 pKa = 9.18IWAEE971 pKa = 4.03YY972 pKa = 10.45SDD974 pKa = 4.44PASGTQSVEE983 pKa = 3.74LSHH986 pKa = 7.18AGNDD990 pKa = 3.41YY991 pKa = 10.95SLPDD995 pKa = 3.96TEE997 pKa = 5.4PGATNIPIEE1006 pKa = 4.29FNADD1010 pKa = 2.91AEE1012 pKa = 4.38ATYY1015 pKa = 11.46NLTYY1019 pKa = 10.14PDD1021 pKa = 3.69AGRR1024 pKa = 11.84LVLKK1028 pKa = 8.35TQYY1031 pKa = 10.25EE1032 pKa = 4.52GSGDD1036 pKa = 3.88DD1037 pKa = 4.97AGLTMLGDD1045 pKa = 3.68STYY1048 pKa = 8.98VTKK1051 pKa = 9.95PAKK1054 pKa = 10.34LYY1056 pKa = 10.01VHH1058 pKa = 6.9SDD1060 pKa = 3.36DD1061 pKa = 4.67TDD1063 pKa = 3.86ADD1065 pKa = 4.27CASGDD1070 pKa = 3.85ATCSRR1075 pKa = 11.84LTAAGDD1081 pKa = 3.74SFNMKK1086 pKa = 9.51VRR1088 pKa = 11.84GACADD1093 pKa = 3.4NSLTPNFRR1101 pKa = 11.84LDD1103 pKa = 5.3NIDD1106 pKa = 3.32LTQNLVAPAGGEE1118 pKa = 3.94SGSINVTNFNIADD1131 pKa = 3.86EE1132 pKa = 4.99DD1133 pKa = 4.0NGEE1136 pKa = 4.12HH1137 pKa = 7.32LINNQTVSEE1146 pKa = 4.31VGVFTFTASLGSGVDD1161 pKa = 3.67YY1162 pKa = 10.9FGEE1165 pKa = 4.18TSIGNTTLNTSDD1177 pKa = 3.48NIGRR1181 pKa = 11.84FSPHH1185 pKa = 6.2HH1186 pKa = 5.47FVVNATEE1193 pKa = 4.07ACNDD1197 pKa = 3.01SFTYY1201 pKa = 10.13SGQPFTVTAEE1211 pKa = 3.93ARR1213 pKa = 11.84NQGDD1217 pKa = 3.55NPTRR1221 pKa = 11.84NYY1223 pKa = 10.35RR1224 pKa = 11.84DD1225 pKa = 3.26GFVRR1229 pKa = 11.84DD1230 pKa = 3.88PVISNAGDD1238 pKa = 3.3TDD1240 pKa = 3.7NFSNNTLGSSEE1251 pKa = 4.01FTTNDD1256 pKa = 3.83GIGEE1260 pKa = 4.26RR1261 pKa = 11.84TDD1263 pKa = 2.9ITYY1266 pKa = 7.6TFPDD1270 pKa = 4.19KK1271 pKa = 10.17KK1272 pKa = 9.15TAPLNITEE1280 pKa = 4.66LRR1282 pKa = 11.84ATDD1285 pKa = 3.5SDD1287 pKa = 4.88GITSDD1292 pKa = 3.49TYY1294 pKa = 11.05TEE1296 pKa = 4.15GEE1298 pKa = 3.81INIRR1302 pKa = 11.84SGRR1305 pKa = 11.84IALDD1309 pKa = 3.19NAYY1312 pKa = 10.23GSEE1315 pKa = 4.06LVDD1318 pKa = 3.65LRR1320 pKa = 11.84VPMVAQYY1327 pKa = 11.32YY1328 pKa = 10.81DD1329 pKa = 3.01GDD1331 pKa = 3.73AFVRR1335 pKa = 11.84NEE1337 pKa = 4.69EE1338 pKa = 4.6DD1339 pKa = 3.05ICDD1342 pKa = 3.71NGVTISLLNPTDD1354 pKa = 3.58TLEE1357 pKa = 4.4VGDD1360 pKa = 4.21GEE1362 pKa = 4.82TRR1364 pKa = 11.84GEE1366 pKa = 4.28TCVQDD1371 pKa = 3.69TDD1373 pKa = 4.47DD1374 pKa = 5.02SGDD1377 pKa = 3.6SGAGCAIAADD1387 pKa = 3.99TDD1389 pKa = 3.54IQYY1392 pKa = 10.54TDD1394 pKa = 3.89APPDD1398 pKa = 3.61AGEE1401 pKa = 3.84YY1402 pKa = 9.45TLYY1405 pKa = 11.08LKK1407 pKa = 10.96APDD1410 pKa = 5.1DD1411 pKa = 4.95DD1412 pKa = 5.62SSFDD1416 pKa = 4.2PDD1418 pKa = 3.66DD1419 pKa = 5.16PIYY1422 pKa = 10.37GTLTVRR1428 pKa = 11.84ADD1430 pKa = 3.21APAYY1434 pKa = 9.78LQYY1437 pKa = 11.42DD1438 pKa = 3.54WDD1440 pKa = 4.64DD1441 pKa = 3.89DD1442 pKa = 3.92ATEE1445 pKa = 4.49EE1446 pKa = 4.34EE1447 pKa = 4.37PAATISFGRR1456 pKa = 11.84YY1457 pKa = 9.0RR1458 pKa = 11.84GDD1460 pKa = 3.36DD1461 pKa = 3.04RR1462 pKa = 11.84VIYY1465 pKa = 9.65WRR1467 pKa = 11.84EE1468 pKa = 3.59KK1469 pKa = 9.64FF1470 pKa = 3.49

Molecular weight: 155.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R8J189|A0A4R8J189_9GAMM Cytochrome c oxidase cbb3-type subunit 2 OS=Thiohalophilus thiocyanatoxydans OX=381308 GN=EDC23_0310 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.2RR12 pKa = 11.84KK13 pKa = 7.57RR14 pKa = 11.84THH16 pKa = 6.05GFRR19 pKa = 11.84ARR21 pKa = 11.84MKK23 pKa = 8.99TRR25 pKa = 11.84GGRR28 pKa = 11.84KK29 pKa = 8.75VLNARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.98GRR39 pKa = 11.84HH40 pKa = 4.92RR41 pKa = 11.84LAVV44 pKa = 3.37

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2775

0

2775

832478

28

2858

300.0

33.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.419 ± 0.058

0.932 ± 0.017

5.899 ± 0.043

6.716 ± 0.056

3.555 ± 0.031

7.527 ± 0.051

2.533 ± 0.025

5.634 ± 0.037

3.658 ± 0.041

10.98 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.51 ± 0.024

3.234 ± 0.033

4.733 ± 0.031

4.557 ± 0.038

6.723 ± 0.045

5.351 ± 0.033

4.895 ± 0.033

6.859 ± 0.044

1.335 ± 0.02

2.95 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski