Streptococcus satellite phage Javan763
Average proteome isoelectric point is 6.93
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 24 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZWG1|A0A4D5ZWG1_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan763 OX=2558859 GN=JavanS763_0014 PE=4 SV=1
MM1 pKa = 7.27 NRR3 pKa = 11.84 LKK5 pKa = 10.53 EE6 pKa = 3.82 LRR8 pKa = 11.84 KK9 pKa = 9.38 RR10 pKa = 11.84 DD11 pKa = 4.0 KK12 pKa = 9.42 ITQVAFAKK20 pKa = 10.85 DD21 pKa = 3.21 NGIPLRR27 pKa = 11.84 TLQSWEE33 pKa = 3.82 NGEE36 pKa = 4.12 NQIKK40 pKa = 9.89 PEE42 pKa = 4.02 KK43 pKa = 9.73 AQQLADD49 pKa = 3.26 IFGVSVGYY57 pKa = 10.64 LLGFNIDD64 pKa = 5.0 DD65 pKa = 3.86 VTEE68 pKa = 4.31 DD69 pKa = 4.36 EE70 pKa = 5.3 INFHH74 pKa = 6.12 NNVMEE79 pKa = 4.32 RR80 pKa = 11.84 MNKK83 pKa = 8.7 EE84 pKa = 3.4 AFIRR88 pKa = 11.84 FLDD91 pKa = 5.22 FITLSDD97 pKa = 3.39 IVLSDD102 pKa = 3.59 KK103 pKa = 10.55 QIEE106 pKa = 4.43 MIFYY110 pKa = 9.99 QLQDD114 pKa = 3.63 LSEE117 pKa = 4.55 LNSDD121 pKa = 3.51 YY122 pKa = 10.75 RR123 pKa = 11.84 YY124 pKa = 8.92 TEE126 pKa = 3.76 TDD128 pKa = 3.32 VEE130 pKa = 4.54 KK131 pKa = 10.49 LKK133 pKa = 11.32 SMYY136 pKa = 10.06 SVKK139 pKa = 10.33 LNYY142 pKa = 9.52 MPTEE146 pKa = 4.01 GLIKK150 pKa = 10.2 ISNILYY156 pKa = 10.27 KK157 pKa = 10.35 EE158 pKa = 4.06 DD159 pKa = 4.34 AIEE162 pKa = 4.05 EE163 pKa = 4.03 QFEE166 pKa = 4.35 QYY168 pKa = 10.9 KK169 pKa = 10.63 KK170 pKa = 10.74 IIDD173 pKa = 3.5
Molecular weight: 20.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.791
IPC2_protein 4.762
IPC_protein 4.66
Toseland 4.507
ProMoST 4.698
Dawson 4.596
Bjellqvist 4.774
Wikipedia 4.469
Rodwell 4.495
Grimsley 4.418
Solomon 4.596
Lehninger 4.546
Nozaki 4.711
DTASelect 4.863
Thurlkill 4.507
EMBOSS 4.482
Sillero 4.774
Patrickios 4.151
IPC_peptide 4.609
IPC2_peptide 4.762
IPC2.peptide.svr19 4.757
Protein with the highest isoelectric point:
>tr|A0A4D5ZWH6|A0A4D5ZWH6_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan763 OX=2558859 GN=JavanS763_0018 PE=4 SV=1
MM1 pKa = 7.12 MPKK4 pKa = 10.19 SNYY7 pKa = 9.53 RR8 pKa = 11.84 EE9 pKa = 3.74 MRR11 pKa = 11.84 FISSLGEE18 pKa = 3.68 KK19 pKa = 10.38 LLATRR24 pKa = 11.84 ILFTVTIANNQQ35 pKa = 3.18
Molecular weight: 4.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.255
IPC2_protein 9.619
IPC_protein 10.145
Toseland 10.482
ProMoST 10.101
Dawson 10.613
Bjellqvist 10.262
Wikipedia 10.774
Rodwell 10.965
Grimsley 10.657
Solomon 10.701
Lehninger 10.687
Nozaki 10.438
DTASelect 10.262
Thurlkill 10.482
EMBOSS 10.877
Sillero 10.526
Patrickios 10.906
IPC_peptide 10.701
IPC2_peptide 8.931
IPC2.peptide.svr19 8.647
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
24
0
24
3415
35
388
142.3
16.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.388 ± 0.378
0.732 ± 0.109
5.886 ± 0.431
7.994 ± 0.561
4.041 ± 0.404
4.451 ± 0.547
1.698 ± 0.293
7.291 ± 0.33
10.044 ± 0.67
10.571 ± 0.446
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.284 ± 0.33
6.53 ± 0.525
2.401 ± 0.232
4.305 ± 0.306
4.539 ± 0.367
6.149 ± 0.498
6.208 ± 0.492
4.246 ± 0.34
0.761 ± 0.134
4.48 ± 0.391
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here