Prochlorococcus phage P-SSM4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Ronodorvirus; Prochlorococcus virus PSSM4

Average proteome isoelectric point is 5.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 221 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q58LP4|Q58LP4_BPPRS Uncharacterized protein OS=Prochlorococcus phage P-SSM4 OX=268747 GN=PSSM4_054 PE=4 SV=1
MM1 pKa = 7.45TFLNDD6 pKa = 2.53IHH8 pKa = 7.64DD9 pKa = 3.94IVEE12 pKa = 4.25EE13 pKa = 4.37GDD15 pKa = 3.17NGTAHH20 pKa = 7.62DD21 pKa = 5.26IIDD24 pKa = 3.67VMFNLLDD31 pKa = 4.14EE32 pKa = 4.69NQLTQLQDD40 pKa = 3.53VIANQYY46 pKa = 9.18PYY48 pKa = 10.03PDD50 pKa = 4.63KK51 pKa = 11.2DD52 pKa = 4.58DD53 pKa = 4.65LFIDD57 pKa = 3.86INNTGGKK64 pKa = 9.13YY65 pKa = 9.97

Molecular weight: 7.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q58LM4|Q58LM4_BPPRS Uncharacterized protein OS=Prochlorococcus phage P-SSM4 OX=268747 GN=PSSM4_074 PE=4 SV=1
MM1 pKa = 7.55AMYY4 pKa = 11.13KK5 pKa = 9.49MGLSEE10 pKa = 5.44IEE12 pKa = 4.36SKK14 pKa = 10.46PKK16 pKa = 9.18KK17 pKa = 8.31TRR19 pKa = 11.84QGNGAHH25 pKa = 6.03TKK27 pKa = 10.24YY28 pKa = 10.79SATARR33 pKa = 11.84NKK35 pKa = 9.71KK36 pKa = 9.11KK37 pKa = 10.44KK38 pKa = 9.43PYY40 pKa = 9.62RR41 pKa = 11.84GQGKK45 pKa = 9.57

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

221

0

221

56763

25

7312

256.8

28.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.008 ± 0.282

0.787 ± 0.084

6.576 ± 0.152

5.819 ± 0.372

4.304 ± 0.12

7.833 ± 0.406

1.582 ± 0.116

6.58 ± 0.156

6.31 ± 0.51

6.975 ± 0.199

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.052 ± 0.215

6.037 ± 0.223

3.717 ± 0.135

3.631 ± 0.094

3.922 ± 0.2

6.941 ± 0.26

7.998 ± 0.626

6.451 ± 0.193

1.168 ± 0.098

4.307 ± 0.147
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski