Brevibacillus centrosporus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Brevibacillus

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5729 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I3RMG7|A0A1I3RMG7_9BACL Putative MFS transporter AGZA family xanthine/uracil permease OS=Brevibacillus centrosporus OX=54910 GN=SAMN05518846_103456 PE=3 SV=1
MM1 pKa = 7.47WLGGFSEE8 pKa = 4.72NVLEE12 pKa = 6.06DD13 pKa = 3.59FDD15 pKa = 4.12YY16 pKa = 10.98WEE18 pKa = 4.72SSTIDD23 pKa = 3.55TYY25 pKa = 11.98NLDD28 pKa = 4.68DD29 pKa = 3.63EE30 pKa = 4.77TANARR35 pKa = 11.84LVFEE39 pKa = 4.14NQVNNEE45 pKa = 3.84IGSDD49 pKa = 3.8FNFWRR54 pKa = 11.84VNSSGDD60 pKa = 3.24AEE62 pKa = 4.08LRR64 pKa = 11.84YY65 pKa = 9.63YY66 pKa = 11.06ANDD69 pKa = 3.74YY70 pKa = 9.6TDD72 pKa = 3.59DD73 pKa = 3.87WYY75 pKa = 11.29GLMTPYY81 pKa = 10.51KK82 pKa = 10.18INSSDD87 pKa = 3.57NYY89 pKa = 10.86VEE91 pKa = 4.33AQYY94 pKa = 11.59GDD96 pKa = 2.93QWDD99 pKa = 3.71RR100 pKa = 11.84AKK102 pKa = 10.61IQLNYY107 pKa = 10.78EE108 pKa = 4.04LMDD111 pKa = 4.28NDD113 pKa = 4.49GMTAANRR120 pKa = 11.84DD121 pKa = 3.27WTVLHH126 pKa = 5.79EE127 pKa = 4.44TGHH130 pKa = 5.48VFSLKK135 pKa = 10.44HH136 pKa = 5.71NPEE139 pKa = 4.46ADD141 pKa = 3.22SVMRR145 pKa = 11.84TVEE148 pKa = 3.84TSFWGNPIFFDD159 pKa = 3.71YY160 pKa = 11.53DD161 pKa = 3.31EE162 pKa = 4.54FTQLDD167 pKa = 4.93LDD169 pKa = 4.06NLDD172 pKa = 3.12YY173 pKa = 10.87RR174 pKa = 11.84YY175 pKa = 10.84

Molecular weight: 20.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I3WPZ8|A0A1I3WPZ8_9BACL Cell shape-determining protein MreC OS=Brevibacillus centrosporus OX=54910 GN=SAMN05518846_108252 PE=3 SV=1
MM1 pKa = 7.18VVRR4 pKa = 11.84SLLRR8 pKa = 11.84VLATSGIVAIIGYY21 pKa = 10.13LMAPRR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84SRR30 pKa = 11.84FAFKK34 pKa = 9.74WNRR37 pKa = 11.84MPFSMRR43 pKa = 11.84DD44 pKa = 3.42MQRR47 pKa = 11.84MMKK50 pKa = 9.74TGRR53 pKa = 11.84KK54 pKa = 9.13LMRR57 pKa = 11.84AVARR61 pKa = 4.2

Molecular weight: 7.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5729

0

5729

1701798

25

1997

297.0

33.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.452 ± 0.036

0.794 ± 0.008

4.839 ± 0.026

6.973 ± 0.037

4.115 ± 0.026

7.358 ± 0.032

2.173 ± 0.015

6.588 ± 0.028

5.435 ± 0.032

10.243 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.917 ± 0.013

3.337 ± 0.019

4.138 ± 0.024

4.227 ± 0.024

5.05 ± 0.026

5.978 ± 0.023

5.436 ± 0.023

7.571 ± 0.025

1.201 ± 0.014

3.174 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski