Amino acid dipepetide frequency for Brevibacillus centrosporus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.228AlaAla: 8.228 ± 0.113
0.795AlaCys: 0.795 ± 0.024
4.165AlaAsp: 4.165 ± 0.053
5.658AlaGlu: 5.658 ± 0.068
3.537AlaPhe: 3.537 ± 0.044
6.645AlaGly: 6.645 ± 0.077
1.659AlaHis: 1.659 ± 0.035
6.015AlaIle: 6.015 ± 0.065
4.889AlaLys: 4.889 ± 0.066
8.54AlaLeu: 8.54 ± 0.068
2.488AlaMet: 2.488 ± 0.04
2.697AlaAsn: 2.697 ± 0.042
2.855AlaPro: 2.855 ± 0.046
3.237AlaGln: 3.237 ± 0.05
3.79AlaArg: 3.79 ± 0.052
5.014AlaSer: 5.014 ± 0.054
4.218AlaThr: 4.218 ± 0.05
6.597AlaVal: 6.597 ± 0.062
0.871AlaTrp: 0.871 ± 0.024
2.617AlaTyr: 2.617 ± 0.042
0.0AlaXaa: 0.0 ± 0.0
Cys
0.546CysAla: 0.546 ± 0.017
0.115CysCys: 0.115 ± 0.01
0.425CysAsp: 0.425 ± 0.015
0.466CysGlu: 0.466 ± 0.018
0.362CysPhe: 0.362 ± 0.015
0.768CysGly: 0.768 ± 0.022
0.212CysHis: 0.212 ± 0.012
0.481CysIle: 0.481 ± 0.016
0.344CysLys: 0.344 ± 0.015
0.815CysLeu: 0.815 ± 0.022
0.227CysMet: 0.227 ± 0.013
0.263CysAsn: 0.263 ± 0.012
0.363CysPro: 0.363 ± 0.016
0.281CysGln: 0.281 ± 0.014
0.44CysArg: 0.44 ± 0.016
0.554CysSer: 0.554 ± 0.017
0.41CysThr: 0.41 ± 0.017
0.498CysVal: 0.498 ± 0.017
0.1CysTrp: 0.1 ± 0.008
0.264CysTyr: 0.264 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
3.875AspAla: 3.875 ± 0.047
0.358AspCys: 0.358 ± 0.013
2.224AspAsp: 2.224 ± 0.052
3.911AspGlu: 3.911 ± 0.053
2.047AspPhe: 2.047 ± 0.034
3.438AspGly: 3.438 ± 0.049
1.075AspHis: 1.075 ± 0.025
3.163AspIle: 3.163 ± 0.049
2.553AspLys: 2.553 ± 0.046
4.952AspLeu: 4.952 ± 0.06
1.344AspMet: 1.344 ± 0.028
1.348AspAsn: 1.348 ± 0.03
2.348AspPro: 2.348 ± 0.037
2.068AspGln: 2.068 ± 0.037
2.677AspArg: 2.677 ± 0.04
2.456AspSer: 2.456 ± 0.039
2.321AspThr: 2.321 ± 0.041
3.843AspVal: 3.843 ± 0.048
0.712AspTrp: 0.712 ± 0.021
1.678AspTyr: 1.678 ± 0.03
0.0AspXaa: 0.0 ± 0.0
Glu
6.219GluAla: 6.219 ± 0.073
0.398GluCys: 0.398 ± 0.015
3.114GluAsp: 3.114 ± 0.053
6.044GluGlu: 6.044 ± 0.094
1.99GluPhe: 1.99 ± 0.035
4.36GluGly: 4.36 ± 0.054
1.446GluHis: 1.446 ± 0.03
4.465GluIle: 4.465 ± 0.059
4.981GluLys: 4.981 ± 0.052
6.97GluLeu: 6.97 ± 0.072
2.251GluMet: 2.251 ± 0.04
2.525GluAsn: 2.525 ± 0.037
2.181GluPro: 2.181 ± 0.043
3.88GluGln: 3.88 ± 0.053
4.284GluArg: 4.284 ± 0.053
3.375GluSer: 3.375 ± 0.044
3.466GluThr: 3.466 ± 0.043
4.942GluVal: 4.942 ± 0.053
0.992GluTrp: 0.992 ± 0.026
1.846GluTyr: 1.846 ± 0.03
0.0GluXaa: 0.0 ± 0.0
Phe
3.506PheAla: 3.506 ± 0.056
0.42PheCys: 0.42 ± 0.016
2.125PheAsp: 2.125 ± 0.038
2.234PheGlu: 2.234 ± 0.041
2.047PhePhe: 2.047 ± 0.037
3.317PheGly: 3.317 ± 0.049
1.043PheHis: 1.043 ± 0.026
2.579PheIle: 2.579 ± 0.044
1.41PheLys: 1.41 ± 0.03
4.593PheLeu: 4.593 ± 0.056
1.002PheMet: 1.002 ± 0.025
1.162PheAsn: 1.162 ± 0.027
1.752PhePro: 1.752 ± 0.034
1.633PheGln: 1.633 ± 0.03
1.946PheArg: 1.946 ± 0.029
2.918PheSer: 2.918 ± 0.044
2.469PheThr: 2.469 ± 0.035
3.152PheVal: 3.152 ± 0.043
0.514PheTrp: 0.514 ± 0.019
1.333PheTyr: 1.333 ± 0.029
0.0PheXaa: 0.0 ± 0.0
Gly
5.694GlyAla: 5.694 ± 0.068
0.746GlyCys: 0.746 ± 0.026
3.255GlyAsp: 3.255 ± 0.045
4.739GlyGlu: 4.739 ± 0.051
3.273GlyPhe: 3.273 ± 0.045
5.314GlyGly: 5.314 ± 0.065
1.508GlyHis: 1.508 ± 0.035
5.708GlyIle: 5.708 ± 0.059
4.895GlyLys: 4.895 ± 0.062
7.136GlyLeu: 7.136 ± 0.074
2.489GlyMet: 2.489 ± 0.046
2.503GlyAsn: 2.503 ± 0.041
2.013GlyPro: 2.013 ± 0.036
2.715GlyGln: 2.715 ± 0.042
3.319GlyArg: 3.319 ± 0.046
4.305GlySer: 4.305 ± 0.051
4.352GlyThr: 4.352 ± 0.051
5.828GlyVal: 5.828 ± 0.06
1.027GlyTrp: 1.027 ± 0.028
2.764GlyTyr: 2.764 ± 0.044
0.0GlyXaa: 0.0 ± 0.0
His
1.73HisAla: 1.73 ± 0.033
0.206HisCys: 0.206 ± 0.011
1.042HisAsp: 1.042 ± 0.024
1.361HisGlu: 1.361 ± 0.033
1.085HisPhe: 1.085 ± 0.022
1.542HisGly: 1.542 ± 0.03
0.61HisHis: 0.61 ± 0.02
1.285HisIle: 1.285 ± 0.03
0.827HisLys: 0.827 ± 0.02
2.326HisLeu: 2.326 ± 0.038
0.577HisMet: 0.577 ± 0.019
0.627HisAsn: 0.627 ± 0.02
1.352HisPro: 1.352 ± 0.027
0.884HisGln: 0.884 ± 0.024
1.1HisArg: 1.1 ± 0.027
1.262HisSer: 1.262 ± 0.03
1.159HisThr: 1.159 ± 0.025
1.721HisVal: 1.721 ± 0.034
0.275HisTrp: 0.275 ± 0.014
0.76HisTyr: 0.76 ± 0.019
0.0HisXaa: 0.0 ± 0.0
Ile
6.204IleAla: 6.204 ± 0.066
0.655IleCys: 0.655 ± 0.019
3.382IleAsp: 3.382 ± 0.05
4.217IleGlu: 4.217 ± 0.059
2.395IlePhe: 2.395 ± 0.042
5.612IleGly: 5.612 ± 0.076
1.553IleHis: 1.553 ± 0.034
3.777IleIle: 3.777 ± 0.052
2.783IleLys: 2.783 ± 0.045
6.285IleLeu: 6.285 ± 0.072
1.608IleMet: 1.608 ± 0.029
2.188IleAsn: 2.188 ± 0.039
3.261IlePro: 3.261 ± 0.05
2.562IleGln: 2.562 ± 0.043
3.588IleArg: 3.588 ± 0.051
4.318IleSer: 4.318 ± 0.053
3.645IleThr: 3.645 ± 0.049
5.371IleVal: 5.371 ± 0.064
0.66IleTrp: 0.66 ± 0.02
1.819IleTyr: 1.819 ± 0.037
0.0IleXaa: 0.0 ± 0.0
Lys
4.438LysAla: 4.438 ± 0.068
0.24LysCys: 0.24 ± 0.013
2.673LysAsp: 2.673 ± 0.051
4.685LysGlu: 4.685 ± 0.051
1.297LysPhe: 1.297 ± 0.027
3.895LysGly: 3.895 ± 0.051
1.059LysHis: 1.059 ± 0.026
3.1LysIle: 3.1 ± 0.041
3.763LysLys: 3.763 ± 0.056
5.156LysLeu: 5.156 ± 0.054
1.759LysMet: 1.759 ± 0.037
1.939LysAsn: 1.939 ± 0.035
2.245LysPro: 2.245 ± 0.037
2.881LysGln: 2.881 ± 0.047
3.227LysArg: 3.227 ± 0.042
2.853LysSer: 2.853 ± 0.041
2.932LysThr: 2.932 ± 0.042
3.866LysVal: 3.866 ± 0.051
0.815LysTrp: 0.815 ± 0.022
1.531LysTyr: 1.531 ± 0.033
0.0LysXaa: 0.0 ± 0.0
Leu
9.21LeuAla: 9.21 ± 0.089
0.853LeuCys: 0.853 ± 0.023
4.989LeuAsp: 4.989 ± 0.055
6.521LeuGlu: 6.521 ± 0.068
4.767LeuPhe: 4.767 ± 0.071
7.317LeuGly: 7.317 ± 0.078
2.292LeuHis: 2.292 ± 0.042
6.367LeuIle: 6.367 ± 0.066
4.736LeuLys: 4.736 ± 0.056
11.283LeuLeu: 11.283 ± 0.132
2.639LeuMet: 2.639 ± 0.042
3.144LeuAsn: 3.144 ± 0.048
4.663LeuPro: 4.663 ± 0.051
4.449LeuGln: 4.449 ± 0.058
5.071LeuArg: 5.071 ± 0.057
6.934LeuSer: 6.934 ± 0.061
5.864LeuThr: 5.864 ± 0.057
7.263LeuVal: 7.263 ± 0.066
0.982LeuTrp: 0.982 ± 0.027
3.082LeuTyr: 3.082 ± 0.041
0.0LeuXaa: 0.0 ± 0.0
Met
2.517MetAla: 2.517 ± 0.039
0.174MetCys: 0.174 ± 0.011
1.5MetAsp: 1.5 ± 0.028
2.079MetGlu: 2.079 ± 0.035
1.011MetPhe: 1.011 ± 0.025
2.099MetGly: 2.099 ± 0.035
0.485MetHis: 0.485 ± 0.017
2.03MetIle: 2.03 ± 0.035
1.971MetLys: 1.971 ± 0.033
2.88MetLeu: 2.88 ± 0.044
0.85MetMet: 0.85 ± 0.024
1.337MetAsn: 1.337 ± 0.028
1.145MetPro: 1.145 ± 0.025
1.135MetGln: 1.135 ± 0.026
1.482MetArg: 1.482 ± 0.027
1.792MetSer: 1.792 ± 0.03
1.712MetThr: 1.712 ± 0.03
1.97MetVal: 1.97 ± 0.037
0.254MetTrp: 0.254 ± 0.013
0.745MetTyr: 0.745 ± 0.021
0.0MetXaa: 0.0 ± 0.0
Asn
2.601AsnAla: 2.601 ± 0.039
0.26AsnCys: 0.26 ± 0.012
1.517AsnAsp: 1.517 ± 0.031
2.24AsnGlu: 2.24 ± 0.034
1.131AsnPhe: 1.131 ± 0.028
2.792AsnGly: 2.792 ± 0.048
0.79AsnHis: 0.79 ± 0.021
2.075AsnIle: 2.075 ± 0.042
1.74AsnLys: 1.74 ± 0.031
3.168AsnLeu: 3.168 ± 0.049
0.958AsnMet: 0.958 ± 0.024
1.176AsnAsn: 1.176 ± 0.029
1.987AsnPro: 1.987 ± 0.034
1.603AsnGln: 1.603 ± 0.036
1.973AsnArg: 1.973 ± 0.038
1.654AsnSer: 1.654 ± 0.037
1.692AsnThr: 1.692 ± 0.031
2.558AsnVal: 2.558 ± 0.036
0.439AsnTrp: 0.439 ± 0.015
1.017AsnTyr: 1.017 ± 0.028
0.0AsnXaa: 0.0 ± 0.0
Pro
3.473ProAla: 3.473 ± 0.055
0.22ProCys: 0.22 ± 0.011
2.419ProAsp: 2.419 ± 0.043
3.174ProGlu: 3.174 ± 0.039
2.088ProPhe: 2.088 ± 0.033
2.933ProGly: 2.933 ± 0.041
1.0ProHis: 1.0 ± 0.023
2.739ProIle: 2.739 ± 0.044
1.931ProLys: 1.931 ± 0.039
4.191ProLeu: 4.191 ± 0.05
1.037ProMet: 1.037 ± 0.024
1.404ProAsn: 1.404 ± 0.029
1.39ProPro: 1.39 ± 0.034
1.554ProGln: 1.554 ± 0.034
1.598ProArg: 1.598 ± 0.033
2.506ProSer: 2.506 ± 0.039
2.264ProThr: 2.264 ± 0.042
3.435ProVal: 3.435 ± 0.044
0.442ProTrp: 0.442 ± 0.016
1.581ProTyr: 1.581 ± 0.035
0.0ProXaa: 0.0 ± 0.0
Gln
3.879GlnAla: 3.879 ± 0.054
0.189GlnCys: 0.189 ± 0.01
1.76GlnAsp: 1.76 ± 0.032
3.242GlnGlu: 3.242 ± 0.047
1.52GlnPhe: 1.52 ± 0.029
2.567GlnGly: 2.567 ± 0.045
0.816GlnHis: 0.816 ± 0.022
2.756GlnIle: 2.756 ± 0.044
2.628GlnLys: 2.628 ± 0.038
4.521GlnLeu: 4.521 ± 0.06
1.392GlnMet: 1.392 ± 0.029
1.424GlnAsn: 1.424 ± 0.028
1.746GlnPro: 1.746 ± 0.035
2.178GlnGln: 2.178 ± 0.045
2.074GlnArg: 2.074 ± 0.035
2.376GlnSer: 2.376 ± 0.041
2.276GlnThr: 2.276 ± 0.04
3.173GlnVal: 3.173 ± 0.041
0.551GlnTrp: 0.551 ± 0.02
1.204GlnTyr: 1.204 ± 0.024
0.0GlnXaa: 0.0 ± 0.0
Arg
3.573ArgAla: 3.573 ± 0.047
0.388ArgCys: 0.388 ± 0.015
2.507ArgAsp: 2.507 ± 0.04
4.154ArgGlu: 4.154 ± 0.056
2.286ArgPhe: 2.286 ± 0.035
3.044ArgGly: 3.044 ± 0.041
1.091ArgHis: 1.091 ± 0.026
3.538ArgIle: 3.538 ± 0.05
3.031ArgLys: 3.031 ± 0.045
5.393ArgLeu: 5.393 ± 0.062
1.732ArgMet: 1.732 ± 0.03
1.752ArgAsn: 1.752 ± 0.038
1.788ArgPro: 1.788 ± 0.037
2.273ArgGln: 2.273 ± 0.038
2.577ArgArg: 2.577 ± 0.047
2.832ArgSer: 2.832 ± 0.045
2.496ArgThr: 2.496 ± 0.04
3.643ArgVal: 3.643 ± 0.046
0.596ArgTrp: 0.596 ± 0.021
1.806ArgTyr: 1.806 ± 0.033
0.0ArgXaa: 0.0 ± 0.0
Ser
4.533SerAla: 4.533 ± 0.054
0.464SerCys: 0.464 ± 0.016
2.66SerAsp: 2.66 ± 0.043
3.596SerGlu: 3.596 ± 0.05
3.054SerPhe: 3.054 ± 0.053
4.727SerGly: 4.727 ± 0.059
1.359SerHis: 1.359 ± 0.031
4.144SerIle: 4.144 ± 0.053
2.933SerLys: 2.933 ± 0.048
6.493SerLeu: 6.493 ± 0.065
1.913SerMet: 1.913 ± 0.033
1.779SerAsn: 1.779 ± 0.033
2.534SerPro: 2.534 ± 0.043
2.266SerGln: 2.266 ± 0.04
2.916SerArg: 2.916 ± 0.04
3.899SerSer: 3.899 ± 0.052
3.235SerThr: 3.235 ± 0.043
4.421SerVal: 4.421 ± 0.056
0.765SerTrp: 0.765 ± 0.02
2.088SerTyr: 2.088 ± 0.039
0.0SerXaa: 0.0 ± 0.0
Thr
4.655ThrAla: 4.655 ± 0.058
0.404ThrCys: 0.404 ± 0.018
2.586ThrAsp: 2.586 ± 0.044
3.322ThrGlu: 3.322 ± 0.046
2.4ThrPhe: 2.4 ± 0.036
4.536ThrGly: 4.536 ± 0.053
1.19ThrHis: 1.19 ± 0.025
3.918ThrIle: 3.918 ± 0.046
2.623ThrLys: 2.623 ± 0.041
5.521ThrLeu: 5.521 ± 0.054
1.47ThrMet: 1.47 ± 0.031
1.798ThrAsn: 1.798 ± 0.034
2.645ThrPro: 2.645 ± 0.041
1.778ThrGln: 1.778 ± 0.032
2.342ThrArg: 2.342 ± 0.037
3.289ThrSer: 3.289 ± 0.048
2.994ThrThr: 2.994 ± 0.042
4.392ThrVal: 4.392 ± 0.05
0.633ThrTrp: 0.633 ± 0.022
1.866ThrTyr: 1.866 ± 0.032
0.0ThrXaa: 0.0 ± 0.0
Val
6.464ValAla: 6.464 ± 0.069
0.655ValCys: 0.655 ± 0.02
3.835ValAsp: 3.835 ± 0.048
5.135ValGlu: 5.135 ± 0.051
2.982ValPhe: 2.982 ± 0.043
5.342ValGly: 5.342 ± 0.064
1.577ValHis: 1.577 ± 0.029
5.184ValIle: 5.184 ± 0.057
4.11ValLys: 4.11 ± 0.057
7.429ValLeu: 7.429 ± 0.078
2.187ValMet: 2.187 ± 0.042
2.748ValAsn: 2.748 ± 0.044
3.271ValPro: 3.271 ± 0.051
2.819ValGln: 2.819 ± 0.04
3.623ValArg: 3.623 ± 0.047
4.899ValSer: 4.899 ± 0.059
4.524ValThr: 4.524 ± 0.052
5.92ValVal: 5.92 ± 0.071
0.797ValTrp: 0.797 ± 0.024
2.211ValTyr: 2.211 ± 0.042
0.0ValXaa: 0.0 ± 0.0
Trp
0.784TrpAla: 0.784 ± 0.024
0.093TrpCys: 0.093 ± 0.007
0.547TrpAsp: 0.547 ± 0.02
0.874TrpGlu: 0.874 ± 0.024
0.521TrpPhe: 0.521 ± 0.017
0.828TrpGly: 0.828 ± 0.025
0.224TrpHis: 0.224 ± 0.013
0.831TrpIle: 0.831 ± 0.025
0.807TrpLys: 0.807 ± 0.021
1.41TrpLeu: 1.41 ± 0.033
0.463TrpMet: 0.463 ± 0.018
0.497TrpAsn: 0.497 ± 0.017
0.338TrpPro: 0.338 ± 0.013
0.528TrpGln: 0.528 ± 0.018
0.611TrpArg: 0.611 ± 0.02
0.689TrpSer: 0.689 ± 0.023
0.604TrpThr: 0.604 ± 0.019
0.816TrpVal: 0.816 ± 0.023
0.181TrpTrp: 0.181 ± 0.01
0.368TrpTyr: 0.368 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.385TyrAla: 2.385 ± 0.037
0.304TyrCys: 0.304 ± 0.014
1.665TyrAsp: 1.665 ± 0.038
2.076TyrGlu: 2.076 ± 0.038
1.361TyrPhe: 1.361 ± 0.031
2.507TyrGly: 2.507 ± 0.04
0.74TyrHis: 0.74 ± 0.022
1.727TyrIle: 1.727 ± 0.031
1.401TyrLys: 1.401 ± 0.032
3.362TyrLeu: 3.362 ± 0.046
0.783TyrMet: 0.783 ± 0.023
1.059TyrAsn: 1.059 ± 0.027
1.503TyrPro: 1.503 ± 0.032
1.547TyrGln: 1.547 ± 0.032
1.863TyrArg: 1.863 ± 0.035
1.855TyrSer: 1.855 ± 0.036
1.729TyrThr: 1.729 ± 0.033
2.304TyrVal: 2.304 ± 0.036
0.409TyrTrp: 0.409 ± 0.015
1.163TyrTyr: 1.163 ± 0.03
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5729 proteins (1701798 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski