Serinibacter salmoneus
Average proteome isoelectric point is 6.05
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2863 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2A9D1Y5|A0A2A9D1Y5_9MICO Transcriptional regulator AbiEi antitoxin Type IV TA system OS=Serinibacter salmoneus OX=556530 GN=ATL40_2294 PE=4 SV=1
MM1 pKa = 7.55 SSPRR5 pKa = 11.84 ATTRR9 pKa = 11.84 IRR11 pKa = 11.84 RR12 pKa = 11.84 TRR14 pKa = 11.84 PALIAACTALLALATACTPEE34 pKa = 6.0 DD35 pKa = 4.47 GEE37 pKa = 5.02 SPDD40 pKa = 4.19 PTPSAAPEE48 pKa = 4.12 DD49 pKa = 4.0 ATTDD53 pKa = 3.51 GSITGDD59 pKa = 3.47 LTLSDD64 pKa = 5.21 LGPDD68 pKa = 3.25 GDD70 pKa = 4.04 TTVFPGGSAQAAVDD84 pKa = 3.59 QVEE87 pKa = 4.25 QVVDD91 pKa = 3.62 QVLADD96 pKa = 3.6 TGVPGAAVAIVTGDD110 pKa = 3.27 EE111 pKa = 3.92 VLYY114 pKa = 10.42 TGGFGVRR121 pKa = 11.84 DD122 pKa = 3.94 LTTGEE127 pKa = 4.19 PVDD130 pKa = 4.14 AEE132 pKa = 4.65 TVFQIASVSKK142 pKa = 10.3 SVGATVVATQVTDD155 pKa = 4.91 GVVSWDD161 pKa = 4.38 DD162 pKa = 3.46 PSHH165 pKa = 6.38 TYY167 pKa = 10.61 LPEE170 pKa = 6.14 LEE172 pKa = 5.78 LSDD175 pKa = 3.77 AWVSEE180 pKa = 3.91 HH181 pKa = 5.53 VTIGDD186 pKa = 4.4 LYY188 pKa = 9.69 SHH190 pKa = 6.68 RR191 pKa = 11.84 TGLPHH196 pKa = 7.55 AAGDD200 pKa = 3.87 LLEE203 pKa = 5.72 DD204 pKa = 3.03 IGYY207 pKa = 10.04 DD208 pKa = 2.94 RR209 pKa = 11.84 TEE211 pKa = 3.44 ILQRR215 pKa = 11.84 LVYY218 pKa = 10.41 QPLNPFRR225 pKa = 11.84 TSYY228 pKa = 11.28 AYY230 pKa = 10.53 ANYY233 pKa = 7.83 GTTAGGEE240 pKa = 4.36 AVAHH244 pKa = 6.11 AAGTTWEE251 pKa = 5.04 DD252 pKa = 3.31 LSQDD256 pKa = 3.41 ALYY259 pKa = 10.73 DD260 pKa = 3.93 PLGMTSTSSRR270 pKa = 11.84 HH271 pKa = 4.5 VDD273 pKa = 3.18 YY274 pKa = 10.95 EE275 pKa = 3.94 AAANRR280 pKa = 11.84 ATLHH284 pKa = 6.18 MYY286 pKa = 10.68 LGDD289 pKa = 3.84 GEE291 pKa = 4.61 FEE293 pKa = 4.36 HH294 pKa = 7.27 VAEE297 pKa = 5.25 RR298 pKa = 11.84 DD299 pKa = 3.61 PDD301 pKa = 3.87 PQSPAGGVSSTASDD315 pKa = 3.67 LALWLQLLLNDD326 pKa = 4.07 GVGPQGQEE334 pKa = 3.93 LIAPEE339 pKa = 4.33 ALQPAITPQSFSAPASVPQARR360 pKa = 11.84 SGSYY364 pKa = 10.45 GYY366 pKa = 10.66 GFNVGVTAGGQPKK379 pKa = 9.93 LSHH382 pKa = 6.46 SGAFVIGTGTSFVVLPGLDD401 pKa = 3.45 VAVVALTNGGPVGAAEE417 pKa = 5.62 AITASLADD425 pKa = 3.65 LAEE428 pKa = 4.44 YY429 pKa = 11.3 GEE431 pKa = 4.42 VTRR434 pKa = 11.84 DD435 pKa = 2.76 WAAGYY440 pKa = 9.06 GALFEE445 pKa = 5.33 PYY447 pKa = 9.07 HH448 pKa = 7.1 APVGDD453 pKa = 4.53 LAEE456 pKa = 4.22 QDD458 pKa = 3.98 PPADD462 pKa = 3.21 AAAPQDD468 pKa = 3.44 LATYY472 pKa = 8.6 TGAWEE477 pKa = 3.63 NDD479 pKa = 3.59 YY480 pKa = 11.25 YY481 pKa = 11.61 GEE483 pKa = 4.24 AVVSVAGEE491 pKa = 4.04 TLVAEE496 pKa = 5.01 LGPDD500 pKa = 3.1 GGYY503 pKa = 10.41 VLEE506 pKa = 4.26 LQPWDD511 pKa = 3.34 GDD513 pKa = 3.65 TFAFVPTGEE522 pKa = 4.05 NAPLGSLSSATFTVPDD538 pKa = 3.63 GTARR542 pKa = 11.84 AEE544 pKa = 4.14 TMTLEE549 pKa = 4.35 FFDD552 pKa = 3.94 ATGLGTWTRR561 pKa = 11.84 ANN563 pKa = 3.4
Molecular weight: 58.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.705
IPC2_protein 3.834
IPC_protein 3.859
Toseland 3.643
ProMoST 4.012
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.757
Rodwell 3.681
Grimsley 3.554
Solomon 3.834
Lehninger 3.795
Nozaki 3.948
DTASelect 4.177
Thurlkill 3.681
EMBOSS 3.77
Sillero 3.973
Patrickios 1.163
IPC_peptide 3.834
IPC2_peptide 3.961
IPC2.peptide.svr19 3.87
Protein with the highest isoelectric point:
>tr|A0A2A9D417|A0A2A9D417_9MICO DNA invertase Pin-like site-specific DNA recombinase OS=Serinibacter salmoneus OX=556530 GN=ATL40_2312 PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.42 KK7 pKa = 8.47 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.22 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.87 KK16 pKa = 9.64 HH17 pKa = 5.77 RR18 pKa = 11.84 KK19 pKa = 7.85 LLRR22 pKa = 11.84 RR23 pKa = 11.84 TRR25 pKa = 11.84 HH26 pKa = 3.6 QRR28 pKa = 11.84 RR29 pKa = 11.84 NKK31 pKa = 9.78 KK32 pKa = 9.85
Molecular weight: 4.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.507
IPC2_protein 11.184
IPC_protein 12.749
Toseland 12.925
ProMoST 13.408
Dawson 12.925
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.735
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.457
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.111
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2863
0
2863
984522
32
2222
343.9
36.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.292 ± 0.069
0.552 ± 0.01
5.786 ± 0.04
6.147 ± 0.053
2.519 ± 0.028
9.25 ± 0.04
2.097 ± 0.025
3.727 ± 0.028
1.385 ± 0.026
10.27 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.836 ± 0.019
1.595 ± 0.022
5.772 ± 0.033
2.895 ± 0.027
7.595 ± 0.051
5.454 ± 0.033
6.341 ± 0.043
9.082 ± 0.047
1.549 ± 0.023
1.857 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here