bacterium J10(2018)

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2759 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3L7ZYG1|A0A3L7ZYG1_9BACT Large-conductance mechanosensitive channel OS=bacterium J10(2018) OX=2320122 GN=mscL PE=3 SV=1
MM1 pKa = 7.38KK2 pKa = 10.67LEE4 pKa = 5.19DD5 pKa = 3.47IKK7 pKa = 11.23ATIDD11 pKa = 3.48SYY13 pKa = 11.63FDD15 pKa = 4.09SISEE19 pKa = 3.86EE20 pKa = 4.24DD21 pKa = 4.89FFDD24 pKa = 5.33LLTNEE29 pKa = 4.02YY30 pKa = 10.73NMPIVYY36 pKa = 10.31DD37 pKa = 3.65IDD39 pKa = 4.41LSPLKK44 pKa = 10.6SDD46 pKa = 3.0IGYY49 pKa = 10.64GDD51 pKa = 3.8VLVEE55 pKa = 4.64LDD57 pKa = 3.96NITYY61 pKa = 10.34EE62 pKa = 4.43PIQIKK67 pKa = 10.5DD68 pKa = 3.43LFINEE73 pKa = 4.03IVKK76 pKa = 9.11VVPPHH81 pKa = 5.44KK82 pKa = 9.46TYY84 pKa = 11.12EE85 pKa = 4.2FVSNDD90 pKa = 3.79DD91 pKa = 3.49SCISYY96 pKa = 10.66SLSFAAA102 pKa = 5.75

Molecular weight: 11.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3L8A5K1|A0A3L8A5K1_9BACT TonB family protein OS=bacterium J10(2018) OX=2320122 GN=D7V95_02670 PE=3 SV=1
MM1 pKa = 7.34RR2 pKa = 11.84THH4 pKa = 7.14IFYY7 pKa = 9.11STRR10 pKa = 11.84DD11 pKa = 3.28HH12 pKa = 7.54DD13 pKa = 4.78FLEE16 pKa = 4.96ACQSVLKK23 pKa = 10.32QNPDD27 pKa = 3.14RR28 pKa = 11.84QFDD31 pKa = 3.82SLLALSIAVSRR42 pKa = 11.84SQAPSYY48 pKa = 10.48YY49 pKa = 10.14VEE51 pKa = 4.29PDD53 pKa = 3.09YY54 pKa = 11.15AYY56 pKa = 10.6RR57 pKa = 11.84IVLAIRR63 pKa = 11.84RR64 pKa = 11.84NGGIKK69 pKa = 9.58GTGTTQMKK77 pKa = 8.75WAEE80 pKa = 4.39FYY82 pKa = 10.66RR83 pKa = 11.84KK84 pKa = 9.91VMTRR88 pKa = 11.84MKK90 pKa = 10.03RR91 pKa = 11.84RR92 pKa = 11.84KK93 pKa = 9.99GEE95 pKa = 4.0SLRR98 pKa = 11.84DD99 pKa = 3.67AVIHH103 pKa = 5.33VAAFEE108 pKa = 4.05PAGSFFISPEE118 pKa = 3.96CGAKK122 pKa = 9.6ILRR125 pKa = 11.84THH127 pKa = 6.66LRR129 pKa = 11.84RR130 pKa = 11.84RR131 pKa = 11.84TCALTTLLSPALVIRR146 pKa = 11.84STRR149 pKa = 11.84RR150 pKa = 11.84RR151 pKa = 11.84FRR153 pKa = 11.84HH154 pKa = 5.72NIKK157 pKa = 10.37HH158 pKa = 5.49

Molecular weight: 18.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2759

0

2759

975351

20

2465

353.5

39.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.866 ± 0.053

1.255 ± 0.018

6.39 ± 0.039

5.953 ± 0.044

4.175 ± 0.034

6.977 ± 0.035

1.94 ± 0.019

6.946 ± 0.046

5.717 ± 0.043

8.636 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.846 ± 0.018

4.865 ± 0.034

3.999 ± 0.025

2.793 ± 0.026

5.336 ± 0.034

6.367 ± 0.034

5.599 ± 0.04

6.76 ± 0.037

1.291 ± 0.021

4.289 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski