Amino acid dipepetide frequency for bacterium J10(2018)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.91AlaAla: 6.91 ± 0.127	0.942AlaCys: 0.942 ± 0.033	5.377AlaAsp: 5.377 ± 0.075	4.881AlaGlu: 4.881 ± 0.077	2.943AlaPhe: 2.943 ± 0.062	5.809AlaGly: 5.809 ± 0.096	1.313AlaHis: 1.313 ± 0.034	5.279AlaIle: 5.279 ± 0.075	4.157AlaLys: 4.157 ± 0.067	7.408AlaLeu: 7.408 ± 0.098	2.352AlaMet: 2.352 ± 0.047	2.906AlaAsn: 2.906 ± 0.052	2.91AlaPro: 2.91 ± 0.067	2.492AlaGln: 2.492 ± 0.055	3.861AlaArg: 3.861 ± 0.072	4.915AlaSer: 4.915 ± 0.074	4.328AlaThr: 4.328 ± 0.067	6.049AlaVal: 6.049 ± 0.095	0.903AlaTrp: 0.903 ± 0.029	2.925AlaTyr: 2.925 ± 0.054	0.0AlaXaa: 0.0 ± 0.0
Cys	0.938CysAla: 0.938 ± 0.034	0.202CysCys: 0.202 ± 0.015	0.828CysAsp: 0.828 ± 0.03	0.651CysGlu: 0.651 ± 0.026	0.522CysPhe: 0.522 ± 0.023	1.131CysGly: 1.131 ± 0.042	0.334CysHis: 0.334 ± 0.022	0.855CysIle: 0.855 ± 0.034	0.535CysLys: 0.535 ± 0.023	0.942CysLeu: 0.942 ± 0.039	0.323CysMet: 0.323 ± 0.019	0.583CysAsn: 0.583 ± 0.024	0.569CysPro: 0.569 ± 0.027	0.26CysGln: 0.26 ± 0.016	0.876CysArg: 0.876 ± 0.035	0.819CysSer: 0.819 ± 0.032	0.594CysThr: 0.594 ± 0.025	0.876CysVal: 0.876 ± 0.029	0.16CysTrp: 0.16 ± 0.013	0.549CysTyr: 0.549 ± 0.023	0.0CysXaa: 0.0 ± 0.0
Asp	4.84AspAla: 4.84 ± 0.075	0.811AspCys: 0.811 ± 0.03	3.96AspAsp: 3.96 ± 0.073	4.067AspGlu: 4.067 ± 0.068	3.098AspPhe: 3.098 ± 0.06	5.005AspGly: 5.005 ± 0.104	0.886AspHis: 0.886 ± 0.03	5.138AspIle: 5.138 ± 0.075	3.838AspLys: 3.838 ± 0.072	4.635AspLeu: 4.635 ± 0.073	2.055AspMet: 2.055 ± 0.05	3.56AspAsn: 3.56 ± 0.064	2.255AspPro: 2.255 ± 0.055	1.098AspGln: 1.098 ± 0.038	3.476AspArg: 3.476 ± 0.069	3.835AspSer: 3.835 ± 0.079	3.449AspThr: 3.449 ± 0.063	3.988AspVal: 3.988 ± 0.063	0.821AspTrp: 0.821 ± 0.031	3.085AspTyr: 3.085 ± 0.059	0.0AspXaa: 0.0 ± 0.0
Glu	5.08GluAla: 5.08 ± 0.089	0.645GluCys: 0.645 ± 0.032	2.959GluAsp: 2.959 ± 0.056	3.957GluGlu: 3.957 ± 0.072	2.364GluPhe: 2.364 ± 0.053	3.601GluGly: 3.601 ± 0.068	1.229GluHis: 1.229 ± 0.036	4.285GluIle: 4.285 ± 0.076	3.966GluLys: 3.966 ± 0.078	5.796GluLeu: 5.796 ± 0.098	1.718GluMet: 1.718 ± 0.04	3.028GluAsn: 3.028 ± 0.057	1.969GluPro: 1.969 ± 0.058	1.992GluGln: 1.992 ± 0.052	3.435GluArg: 3.435 ± 0.067	3.079GluSer: 3.079 ± 0.062	2.815GluThr: 2.815 ± 0.057	4.017GluVal: 4.017 ± 0.071	0.896GluTrp: 0.896 ± 0.028	2.705GluTyr: 2.705 ± 0.053	0.0GluXaa: 0.0 ± 0.0
Phe	2.994PheAla: 2.994 ± 0.052	0.562PheCys: 0.562 ± 0.024	2.935PheAsp: 2.935 ± 0.062	2.087PheGlu: 2.087 ± 0.049	1.896PhePhe: 1.896 ± 0.056	3.134PheGly: 3.134 ± 0.053	0.813PheHis: 0.813 ± 0.029	3.028PheIle: 3.028 ± 0.065	2.19PheLys: 2.19 ± 0.047	3.214PheLeu: 3.214 ± 0.071	1.238PheMet: 1.238 ± 0.039	2.47PheAsn: 2.47 ± 0.06	1.607PhePro: 1.607 ± 0.041	0.922PheGln: 0.922 ± 0.034	2.154PheArg: 2.154 ± 0.046	3.052PheSer: 3.052 ± 0.062	2.598PheThr: 2.598 ± 0.059	2.622PheVal: 2.622 ± 0.052	0.505PheTrp: 0.505 ± 0.025	1.728PheTyr: 1.728 ± 0.041	0.0PheXaa: 0.0 ± 0.0
Gly	5.282GlyAla: 5.282 ± 0.088	1.088GlyCys: 1.088 ± 0.035	4.211GlyAsp: 4.211 ± 0.068	3.924GlyGlu: 3.924 ± 0.069	3.042GlyPhe: 3.042 ± 0.057	4.746GlyGly: 4.746 ± 0.092	1.499GlyHis: 1.499 ± 0.044	5.205GlyIle: 5.205 ± 0.078	4.405GlyLys: 4.405 ± 0.07	5.728GlyLeu: 5.728 ± 0.078	2.069GlyMet: 2.069 ± 0.049	3.551GlyAsn: 3.551 ± 0.077	1.583GlyPro: 1.583 ± 0.038	1.908GlyGln: 1.908 ± 0.046	3.783GlyArg: 3.783 ± 0.071	4.122GlySer: 4.122 ± 0.075	4.061GlyThr: 4.061 ± 0.071	5.068GlyVal: 5.068 ± 0.079	1.076GlyTrp: 1.076 ± 0.044	3.421GlyTyr: 3.421 ± 0.067	0.0GlyXaa: 0.0 ± 0.0
His	1.243HisAla: 1.243 ± 0.038	0.316HisCys: 0.316 ± 0.019	1.339HisAsp: 1.339 ± 0.041	1.077HisGlu: 1.077 ± 0.036	0.866HisPhe: 0.866 ± 0.03	1.486HisGly: 1.486 ± 0.041	0.486HisHis: 0.486 ± 0.025	1.488HisIle: 1.488 ± 0.036	1.03HisLys: 1.03 ± 0.037	1.556HisLeu: 1.556 ± 0.044	0.484HisMet: 0.484 ± 0.022	1.087HisAsn: 1.087 ± 0.034	1.021HisPro: 1.021 ± 0.034	0.496HisGln: 0.496 ± 0.018	1.041HisArg: 1.041 ± 0.036	1.12HisSer: 1.12 ± 0.035	1.022HisThr: 1.022 ± 0.033	1.112HisVal: 1.112 ± 0.034	0.231HisTrp: 0.231 ± 0.015	0.896HisTyr: 0.896 ± 0.031	0.0HisXaa: 0.0 ± 0.0
Ile	6.04IleAla: 6.04 ± 0.094	0.859IleCys: 0.859 ± 0.035	5.07IleAsp: 5.07 ± 0.071	4.329IleGlu: 4.329 ± 0.071	2.722IlePhe: 2.722 ± 0.071	4.716IleGly: 4.716 ± 0.081	1.185IleHis: 1.185 ± 0.036	4.924IleIle: 4.924 ± 0.088	4.307IleLys: 4.307 ± 0.073	5.392IleLeu: 5.392 ± 0.095	1.693IleMet: 1.693 ± 0.044	3.641IleAsn: 3.641 ± 0.069	3.091IlePro: 3.091 ± 0.051	1.612IleGln: 1.612 ± 0.045	3.029IleArg: 3.029 ± 0.056	4.636IleSer: 4.636 ± 0.074	4.101IleThr: 4.101 ± 0.074	4.768IleVal: 4.768 ± 0.08	0.695IleTrp: 0.695 ± 0.026	2.65IleTyr: 2.65 ± 0.054	0.0IleXaa: 0.0 ± 0.0
Lys	4.718LysAla: 4.718 ± 0.076	0.58LysCys: 0.58 ± 0.027	3.254LysAsp: 3.254 ± 0.062	4.115LysGlu: 4.115 ± 0.072	2.055LysPhe: 2.055 ± 0.049	3.859LysGly: 3.859 ± 0.064	1.112LysHis: 1.112 ± 0.033	3.72LysIle: 3.72 ± 0.063	3.734LysLys: 3.734 ± 0.071	4.608LysLeu: 4.608 ± 0.075	1.737LysMet: 1.737 ± 0.044	2.788LysAsn: 2.788 ± 0.061	2.207LysPro: 2.207 ± 0.05	1.738LysGln: 1.738 ± 0.046	3.038LysArg: 3.038 ± 0.066	3.604LysSer: 3.604 ± 0.062	3.207LysThr: 3.207 ± 0.064	3.792LysVal: 3.792 ± 0.07	0.76LysTrp: 0.76 ± 0.03	2.54LysTyr: 2.54 ± 0.049	0.0LysXaa: 0.0 ± 0.0
Leu	6.693LeuAla: 6.693 ± 0.098	1.3LeuCys: 1.3 ± 0.038	5.457LeuAsp: 5.457 ± 0.079	4.434LeuGlu: 4.434 ± 0.068	3.574LeuPhe: 3.574 ± 0.067	5.539LeuGly: 5.539 ± 0.092	1.754LeuHis: 1.754 ± 0.045	5.368LeuIle: 5.368 ± 0.095	5.186LeuLys: 5.186 ± 0.079	7.652LeuLeu: 7.652 ± 0.123	2.442LeuMet: 2.442 ± 0.053	4.258LeuAsn: 4.258 ± 0.069	3.987LeuPro: 3.987 ± 0.069	2.527LeuGln: 2.527 ± 0.061	4.747LeuArg: 4.747 ± 0.078	6.519LeuSer: 6.519 ± 0.102	5.245LeuThr: 5.245 ± 0.076	5.043LeuVal: 5.043 ± 0.086	1.067LeuTrp: 1.067 ± 0.037	3.562LeuTyr: 3.562 ± 0.068	0.0LeuXaa: 0.0 ± 0.0
Met	2.486MetAla: 2.486 ± 0.052	0.311MetCys: 0.311 ± 0.017	1.45MetAsp: 1.45 ± 0.037	1.604MetGlu: 1.604 ± 0.039	1.039MetPhe: 1.039 ± 0.035	1.638MetGly: 1.638 ± 0.043	0.567MetHis: 0.567 ± 0.023	1.604MetIle: 1.604 ± 0.043	2.135MetLys: 2.135 ± 0.046	2.824MetLeu: 2.824 ± 0.057	0.88MetMet: 0.88 ± 0.03	1.371MetAsn: 1.371 ± 0.035	1.42MetPro: 1.42 ± 0.042	0.861MetGln: 0.861 ± 0.031	1.575MetArg: 1.575 ± 0.044	1.999MetSer: 1.999 ± 0.051	1.928MetThr: 1.928 ± 0.041	1.547MetVal: 1.547 ± 0.037	0.386MetTrp: 0.386 ± 0.02	0.842MetTyr: 0.842 ± 0.028	0.0MetXaa: 0.0 ± 0.0
Asn	3.581AsnAla: 3.581 ± 0.069	0.527AsnCys: 0.527 ± 0.026	3.11AsnAsp: 3.11 ± 0.054	2.695AsnGlu: 2.695 ± 0.058	1.979AsnPhe: 1.979 ± 0.048	3.831AsnGly: 3.831 ± 0.071	0.939AsnHis: 0.939 ± 0.033	3.729AsnIle: 3.729 ± 0.07	2.631AsnLys: 2.631 ± 0.053	3.859AsnLeu: 3.859 ± 0.067	1.325AsnMet: 1.325 ± 0.033	2.541AsnAsn: 2.541 ± 0.066	2.592AsnPro: 2.592 ± 0.056	1.167AsnGln: 1.167 ± 0.042	2.788AsnArg: 2.788 ± 0.061	2.817AsnSer: 2.817 ± 0.063	2.666AsnThr: 2.666 ± 0.058	3.21AsnVal: 3.21 ± 0.061	0.605AsnTrp: 0.605 ± 0.027	2.06AsnTyr: 2.06 ± 0.055	0.0AsnXaa: 0.0 ± 0.0
Pro	3.408ProAla: 3.408 ± 0.064	0.391ProCys: 0.391 ± 0.022	3.345ProAsp: 3.345 ± 0.058	3.463ProGlu: 3.463 ± 0.067	1.647ProPhe: 1.647 ± 0.041	2.769ProGly: 2.769 ± 0.055	0.775ProHis: 0.775 ± 0.03	2.21ProIle: 2.21 ± 0.055	1.888ProLys: 1.888 ± 0.044	3.171ProLeu: 3.171 ± 0.058	1.057ProMet: 1.057 ± 0.033	1.411ProAsn: 1.411 ± 0.046	0.938ProPro: 0.938 ± 0.037	1.337ProGln: 1.337 ± 0.04	1.721ProArg: 1.721 ± 0.041	2.641ProSer: 2.641 ± 0.054	1.872ProThr: 1.872 ± 0.048	3.647ProVal: 3.647 ± 0.065	0.529ProTrp: 0.529 ± 0.023	1.768ProTyr: 1.768 ± 0.046	0.0ProXaa: 0.0 ± 0.0
Gln	2.099GlnAla: 2.099 ± 0.059	0.325GlnCys: 0.325 ± 0.019	1.296GlnAsp: 1.296 ± 0.038	1.597GlnGlu: 1.597 ± 0.046	1.21GlnPhe: 1.21 ± 0.035	1.706GlnGly: 1.706 ± 0.045	0.555GlnHis: 0.555 ± 0.024	1.719GlnIle: 1.719 ± 0.042	1.585GlnLys: 1.585 ± 0.047	2.788GlnLeu: 2.788 ± 0.059	0.88GlnMet: 0.88 ± 0.031	1.389GlnAsn: 1.389 ± 0.039	1.15GlnPro: 1.15 ± 0.034	1.246GlnGln: 1.246 ± 0.045	1.605GlnArg: 1.605 ± 0.043	1.899GlnSer: 1.899 ± 0.049	1.497GlnThr: 1.497 ± 0.041	1.628GlnVal: 1.628 ± 0.044	0.499GlnTrp: 0.499 ± 0.023	1.262GlnTyr: 1.262 ± 0.038	0.0GlnXaa: 0.0 ± 0.0
Arg	3.379ArgAla: 3.379 ± 0.061	0.584ArgCys: 0.584 ± 0.026	3.05ArgAsp: 3.05 ± 0.061	3.154ArgGlu: 3.154 ± 0.062	2.46ArgPhe: 2.46 ± 0.052	2.996ArgGly: 2.996 ± 0.06	1.427ArgHis: 1.427 ± 0.038	3.979ArgIle: 3.979 ± 0.071	3.177ArgLys: 3.177 ± 0.062	5.343ArgLeu: 5.343 ± 0.085	1.655ArgMet: 1.655 ± 0.04	2.665ArgAsn: 2.665 ± 0.057	1.992ArgPro: 1.992 ± 0.053	1.954ArgGln: 1.954 ± 0.046	3.604ArgArg: 3.604 ± 0.068	2.764ArgSer: 2.764 ± 0.056	2.537ArgThr: 2.537 ± 0.061	3.088ArgVal: 3.088 ± 0.064	0.788ArgTrp: 0.788 ± 0.031	2.765ArgTyr: 2.765 ± 0.061	0.0ArgXaa: 0.0 ± 0.0
Ser	4.968SerAla: 4.968 ± 0.08	0.84SerCys: 0.84 ± 0.033	4.088SerAsp: 4.088 ± 0.073	3.405SerGlu: 3.405 ± 0.061	2.745SerPhe: 2.745 ± 0.054	5.0SerGly: 5.0 ± 0.087	1.292SerHis: 1.292 ± 0.035	4.396SerIle: 4.396 ± 0.064	2.957SerLys: 2.957 ± 0.062	5.911SerLeu: 5.911 ± 0.069	1.77SerMet: 1.77 ± 0.049	2.463SerAsn: 2.463 ± 0.062	2.609SerPro: 2.609 ± 0.048	1.869SerGln: 1.869 ± 0.044	3.622SerArg: 3.622 ± 0.065	4.056SerSer: 4.056 ± 0.083	3.437SerThr: 3.437 ± 0.058	4.684SerVal: 4.684 ± 0.078	0.848SerTrp: 0.848 ± 0.03	2.706SerTyr: 2.706 ± 0.062	0.0SerXaa: 0.0 ± 0.0
Thr	4.672ThrAla: 4.672 ± 0.077	0.587ThrCys: 0.587 ± 0.026	3.948ThrAsp: 3.948 ± 0.06	3.005ThrGlu: 3.005 ± 0.055	2.449ThrPhe: 2.449 ± 0.055	4.588ThrGly: 4.588 ± 0.079	1.021ThrHis: 1.021 ± 0.034	3.826ThrIle: 3.826 ± 0.07	2.431ThrLys: 2.431 ± 0.064	5.33ThrLeu: 5.33 ± 0.093	1.273ThrMet: 1.273 ± 0.032	2.06ThrAsn: 2.06 ± 0.05	3.032ThrPro: 3.032 ± 0.067	1.399ThrGln: 1.399 ± 0.046	2.487ThrArg: 2.487 ± 0.055	3.498ThrSer: 3.498 ± 0.077	2.994ThrThr: 2.994 ± 0.06	4.529ThrVal: 4.529 ± 0.072	0.63ThrTrp: 0.63 ± 0.026	2.232ThrTyr: 2.232 ± 0.056	0.0ThrXaa: 0.0 ± 0.0
Val	5.562ValAla: 5.562 ± 0.087	0.846ValCys: 0.846 ± 0.03	4.438ValAsp: 4.438 ± 0.068	4.061ValGlu: 4.061 ± 0.076	2.702ValPhe: 2.702 ± 0.059	4.092ValGly: 4.092 ± 0.072	1.07ValHis: 1.07 ± 0.034	4.815ValIle: 4.815 ± 0.086	4.143ValLys: 4.143 ± 0.066	5.528ValLeu: 5.528 ± 0.078	1.943ValMet: 1.943 ± 0.052	3.623ValAsn: 3.623 ± 0.059	2.882ValPro: 2.882 ± 0.05	1.488ValGln: 1.488 ± 0.038	3.27ValArg: 3.27 ± 0.054	4.679ValSer: 4.679 ± 0.077	4.432ValThr: 4.432 ± 0.075	4.666ValVal: 4.666 ± 0.077	0.728ValTrp: 0.728 ± 0.027	2.633ValTyr: 2.633 ± 0.054	0.0ValXaa: 0.0 ± 0.0
Trp	0.794TrpAla: 0.794 ± 0.026	0.193TrpCys: 0.193 ± 0.014	0.807TrpAsp: 0.807 ± 0.028	0.742TrpGlu: 0.742 ± 0.028	0.54TrpPhe: 0.54 ± 0.025	1.022TrpGly: 1.022 ± 0.034	0.312TrpHis: 0.312 ± 0.02	0.897TrpIle: 0.897 ± 0.033	0.702TrpLys: 0.702 ± 0.026	1.224TrpLeu: 1.224 ± 0.034	0.404TrpMet: 0.404 ± 0.019	0.713TrpAsn: 0.713 ± 0.028	0.353TrpPro: 0.353 ± 0.022	0.465TrpGln: 0.465 ± 0.021	0.735TrpArg: 0.735 ± 0.028	0.79TrpSer: 0.79 ± 0.028	0.738TrpThr: 0.738 ± 0.029	0.672TrpVal: 0.672 ± 0.026	0.25TrpTrp: 0.25 ± 0.014	0.558TrpTyr: 0.558 ± 0.026	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.972TyrAla: 2.972 ± 0.059	0.639TyrCys: 0.639 ± 0.027	2.977TyrAsp: 2.977 ± 0.061	2.285TyrGlu: 2.285 ± 0.05	1.897TyrPhe: 1.897 ± 0.042	3.101TyrGly: 3.101 ± 0.067	0.825TyrHis: 0.825 ± 0.03	2.995TyrIle: 2.995 ± 0.05	2.169TyrLys: 2.169 ± 0.052	3.447TyrLeu: 3.447 ± 0.06	1.167TyrMet: 1.167 ± 0.036	2.547TyrAsn: 2.547 ± 0.065	1.822TyrPro: 1.822 ± 0.045	1.102TyrGln: 1.102 ± 0.034	2.516TyrArg: 2.516 ± 0.048	2.821TyrSer: 2.821 ± 0.066	2.473TyrThr: 2.473 ± 0.055	2.595TyrVal: 2.595 ± 0.051	0.534TyrTrp: 0.534 ± 0.026	2.006TyrTyr: 2.006 ± 0.052	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2759 proteins (975351 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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