Arthrobacter phage Qui
Average proteome isoelectric point is 6.31
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 247 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5B8WGD0|A0A5B8WGD0_9CAUD Membrane protein OS=Arthrobacter phage Qui OX=2603260 GN=53 PE=4 SV=1
MM1 pKa = 7.89 TEE3 pKa = 4.33 LTWGNYY9 pKa = 6.95 GEE11 pKa = 4.9 RR12 pKa = 11.84 YY13 pKa = 9.77 YY14 pKa = 10.44 EE15 pKa = 3.98 TGVDD19 pKa = 3.49 RR20 pKa = 11.84 GVLYY24 pKa = 10.94 LPGQDD29 pKa = 2.72 GVVWNGLIAVNEE41 pKa = 4.35 SPSGGEE47 pKa = 3.57 PTPYY51 pKa = 10.76 YY52 pKa = 10.69 LDD54 pKa = 3.32 GVKK57 pKa = 10.41 YY58 pKa = 10.51 LNRR61 pKa = 11.84 PSPEE65 pKa = 3.83 EE66 pKa = 3.57 YY67 pKa = 9.88 RR68 pKa = 11.84 ATIEE72 pKa = 4.21 AYY74 pKa = 8.77 TYY76 pKa = 9.13 PDD78 pKa = 3.31 EE79 pKa = 5.57 FAFCDD84 pKa = 4.36 GSAGIDD90 pKa = 3.7 LEE92 pKa = 4.88 SKK94 pKa = 10.79 GLTATQQGRR103 pKa = 11.84 KK104 pKa = 8.93 PFGFSYY110 pKa = 9.2 RR111 pKa = 11.84 TRR113 pKa = 11.84 IGNDD117 pKa = 3.14 DD118 pKa = 3.82 LATNFGYY125 pKa = 10.7 KK126 pKa = 9.95 LHH128 pKa = 6.52 IVYY131 pKa = 10.19 NALAAPSEE139 pKa = 4.15 KK140 pKa = 10.28 SYY142 pKa = 11.15 QSSSDD147 pKa = 3.59 DD148 pKa = 3.43 PEE150 pKa = 6.61 AISFSWDD157 pKa = 2.69 ITTTPIVVSEE167 pKa = 4.05 AGTYY171 pKa = 10.04 SSHH174 pKa = 7.05 LVLDD178 pKa = 4.97 TKK180 pKa = 7.35 TTWPWVITAVEE191 pKa = 3.92 EE192 pKa = 4.05 LLYY195 pKa = 9.69 GTEE198 pKa = 4.32 DD199 pKa = 3.69 TPPTLPTPQDD209 pKa = 4.2 LIDD212 pKa = 4.61 LFVEE216 pKa = 3.99 NALLKK221 pKa = 9.86 ITDD224 pKa = 4.11 NGDD227 pKa = 3.66 GTWTAEE233 pKa = 4.24 GPDD236 pKa = 4.88 EE237 pKa = 5.18 IIQMLNATEE246 pKa = 4.9 FQISWPSAVYY256 pKa = 10.48 LDD258 pKa = 4.1 EE259 pKa = 4.35 DD260 pKa = 4.62 TYY262 pKa = 11.21 TISSLL267 pKa = 3.61
Molecular weight: 29.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.816
IPC2_protein 3.961
IPC_protein 3.935
Toseland 3.745
ProMoST 4.075
Dawson 3.91
Bjellqvist 4.062
Wikipedia 3.808
Rodwell 3.77
Grimsley 3.643
Solomon 3.897
Lehninger 3.859
Nozaki 4.024
DTASelect 4.215
Thurlkill 3.77
EMBOSS 3.821
Sillero 4.05
Patrickios 0.629
IPC_peptide 3.897
IPC2_peptide 4.037
IPC2.peptide.svr19 3.944
Protein with the highest isoelectric point:
>tr|A0A5B8WHT6|A0A5B8WHT6_9CAUD DNA-directed DNA polymerase OS=Arthrobacter phage Qui OX=2603260 GN=109 PE=4 SV=1
MM1 pKa = 7.11 ARR3 pKa = 11.84 SRR5 pKa = 11.84 KK6 pKa = 9.58 RR7 pKa = 11.84 YY8 pKa = 9.9 GLGKK12 pKa = 10.34 FLFDD16 pKa = 5.99 LILGFLTCGIWWIFLLFKK34 pKa = 10.08 FFRR37 pKa = 11.84 TNSS40 pKa = 3.27
Molecular weight: 4.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.292
IPC2_protein 9.984
IPC_protein 10.979
Toseland 11.169
ProMoST 11.155
Dawson 11.213
Bjellqvist 11.008
Wikipedia 11.506
Rodwell 11.389
Grimsley 11.242
Solomon 11.477
Lehninger 11.418
Nozaki 11.155
DTASelect 11.008
Thurlkill 11.155
EMBOSS 11.594
Sillero 11.169
Patrickios 11.199
IPC_peptide 11.477
IPC2_peptide 10.277
IPC2.peptide.svr19 8.51
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
247
0
247
35067
33
1516
142.0
15.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.933 ± 0.316
0.827 ± 0.076
6.225 ± 0.192
7.337 ± 0.306
3.602 ± 0.115
6.989 ± 0.324
1.996 ± 0.111
6.165 ± 0.158
6.835 ± 0.249
7.785 ± 0.197
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.849 ± 0.105
4.58 ± 0.111
4.155 ± 0.155
3.12 ± 0.112
5.053 ± 0.223
5.889 ± 0.179
6.339 ± 0.222
6.89 ± 0.149
1.848 ± 0.107
3.582 ± 0.179
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here