Bacillus phage 1_ICo-2020

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 75 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G8AKE6|A0A7G8AKE6_9CAUD Transcriptional reulatory protein OS=Bacillus phage 1_ICo-2020 OX=2759272 PE=4 SV=1
MM1 pKa = 7.97RR2 pKa = 11.84INHH5 pKa = 5.28EE6 pKa = 3.94HH7 pKa = 6.33EE8 pKa = 5.16AIKK11 pKa = 10.83DD12 pKa = 3.51RR13 pKa = 11.84AEE15 pKa = 3.75EE16 pKa = 3.93MHH18 pKa = 6.47YY19 pKa = 10.72EE20 pKa = 4.85GYY22 pKa = 10.86DD23 pKa = 3.18SDD25 pKa = 5.61NIFEE29 pKa = 4.56TLLEE33 pKa = 4.52EE34 pKa = 4.45YY35 pKa = 10.55DD36 pKa = 3.68VTEE39 pKa = 5.2DD40 pKa = 3.89EE41 pKa = 4.39LSEE44 pKa = 4.67LIIEE48 pKa = 4.38VEE50 pKa = 3.95HH51 pKa = 6.73RR52 pKa = 11.84VNDD55 pKa = 3.57VPNEE59 pKa = 3.88GFIIEE64 pKa = 4.41GDD66 pKa = 3.6EE67 pKa = 3.96NLYY70 pKa = 11.12GSS72 pKa = 4.22

Molecular weight: 8.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G8AKC4|A0A7G8AKC4_9CAUD Uncharacterized protein OS=Bacillus phage 1_ICo-2020 OX=2759272 PE=4 SV=1
MM1 pKa = 7.31AQNEE5 pKa = 4.73SIRR8 pKa = 11.84VDD10 pKa = 3.02LHH12 pKa = 6.8GLDD15 pKa = 3.88RR16 pKa = 11.84RR17 pKa = 11.84LLRR20 pKa = 11.84AARR23 pKa = 11.84QLEE26 pKa = 4.48DD27 pKa = 3.4YY28 pKa = 7.44KK29 pKa = 11.14TPLRR33 pKa = 11.84RR34 pKa = 11.84SEE36 pKa = 4.05TFMEE40 pKa = 4.01KK41 pKa = 10.43SIGKK45 pKa = 9.45RR46 pKa = 11.84FRR48 pKa = 11.84AAAWRR53 pKa = 11.84PLSEE57 pKa = 4.34TTLRR61 pKa = 11.84WHH63 pKa = 6.02PHH65 pKa = 4.84RR66 pKa = 11.84VGGKK70 pKa = 8.56PLNDD74 pKa = 3.13TGALKK79 pKa = 10.29MSIEE83 pKa = 4.07SGAANKK89 pKa = 9.93LSKK92 pKa = 10.55KK93 pKa = 10.19KK94 pKa = 9.46LTIRR98 pKa = 11.84SGLKK102 pKa = 9.6KK103 pKa = 10.72ANLLHH108 pKa = 6.49HH109 pKa = 7.14GGRR112 pKa = 11.84TSLGTYY118 pKa = 8.96VPSRR122 pKa = 11.84KK123 pKa = 9.03FLYY126 pKa = 10.25FDD128 pKa = 3.58EE129 pKa = 5.18FDD131 pKa = 3.15ARR133 pKa = 11.84MIKK136 pKa = 10.18RR137 pKa = 11.84IFVDD141 pKa = 4.15YY142 pKa = 10.67IEE144 pKa = 4.68EE145 pKa = 4.19VADD148 pKa = 4.49GVDD151 pKa = 3.36PHH153 pKa = 6.55QVV155 pKa = 2.78

Molecular weight: 17.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

75

0

75

14588

36

1084

194.5

22.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.992 ± 0.404

0.953 ± 0.099

5.888 ± 0.211

7.815 ± 0.412

3.695 ± 0.18

6.848 ± 0.253

1.947 ± 0.15

6.485 ± 0.156

7.801 ± 0.366

7.904 ± 0.215

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.612 ± 0.2

5.203 ± 0.27

3.188 ± 0.175

3.763 ± 0.219

4.6 ± 0.247

6.361 ± 0.221

6.121 ± 0.337

6.053 ± 0.202

1.337 ± 0.175

4.435 ± 0.237
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski