Amino acid dipepetide frequency for Bacillus phage 1_ICo-2020

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.593AlaAla: 4.593 ± 0.838
0.48AlaCys: 0.48 ± 0.191
3.016AlaAsp: 3.016 ± 0.444
4.593AlaGlu: 4.593 ± 0.49
3.085AlaPhe: 3.085 ± 0.605
4.25AlaGly: 4.25 ± 0.545
1.097AlaHis: 1.097 ± 0.295
6.65AlaIle: 6.65 ± 0.913
5.553AlaLys: 5.553 ± 0.887
5.759AlaLeu: 5.759 ± 0.568
2.262AlaMet: 2.262 ± 0.493
4.662AlaAsn: 4.662 ± 0.692
2.811AlaPro: 2.811 ± 0.413
1.988AlaGln: 1.988 ± 0.308
3.085AlaArg: 3.085 ± 0.47
4.045AlaSer: 4.045 ± 0.623
4.182AlaThr: 4.182 ± 0.621
3.908AlaVal: 3.908 ± 0.486
1.097AlaTrp: 1.097 ± 0.346
2.811AlaTyr: 2.811 ± 0.516
0.0AlaXaa: 0.0 ± 0.0
Cys
0.48CysAla: 0.48 ± 0.184
0.069CysCys: 0.069 ± 0.062
0.48CysAsp: 0.48 ± 0.235
0.891CysGlu: 0.891 ± 0.185
0.411CysPhe: 0.411 ± 0.14
0.548CysGly: 0.548 ± 0.191
0.274CysHis: 0.274 ± 0.149
0.48CysIle: 0.48 ± 0.163
1.028CysLys: 1.028 ± 0.286
0.823CysLeu: 0.823 ± 0.213
0.0CysMet: 0.0 ± 0.0
0.548CysAsn: 0.548 ± 0.179
0.274CysPro: 0.274 ± 0.141
0.48CysGln: 0.48 ± 0.158
0.411CysArg: 0.411 ± 0.189
0.823CysSer: 0.823 ± 0.251
0.548CysThr: 0.548 ± 0.185
0.548CysVal: 0.548 ± 0.17
0.137CysTrp: 0.137 ± 0.096
0.274CysTyr: 0.274 ± 0.169
0.0CysXaa: 0.0 ± 0.0
Asp
5.004AspAla: 5.004 ± 0.871
0.617AspCys: 0.617 ± 0.199
2.125AspAsp: 2.125 ± 0.332
3.976AspGlu: 3.976 ± 0.55
2.331AspPhe: 2.331 ± 0.353
4.045AspGly: 4.045 ± 0.589
1.303AspHis: 1.303 ± 0.294
4.525AspIle: 4.525 ± 0.571
5.004AspLys: 5.004 ± 0.645
4.319AspLeu: 4.319 ± 0.586
1.097AspMet: 1.097 ± 0.23
2.948AspAsn: 2.948 ± 0.52
1.988AspPro: 1.988 ± 0.384
1.234AspGln: 1.234 ± 0.318
3.565AspArg: 3.565 ± 0.572
3.839AspSer: 3.839 ± 0.586
3.222AspThr: 3.222 ± 0.68
4.182AspVal: 4.182 ± 0.502
0.686AspTrp: 0.686 ± 0.245
2.879AspTyr: 2.879 ± 0.462
0.0AspXaa: 0.0 ± 0.0
Glu
5.073GluAla: 5.073 ± 0.567
0.96GluCys: 0.96 ± 0.246
4.25GluAsp: 4.25 ± 0.594
7.335GluGlu: 7.335 ± 1.011
2.948GluPhe: 2.948 ± 0.425
5.21GluGly: 5.21 ± 0.585
1.714GluHis: 1.714 ± 0.366
5.004GluIle: 5.004 ± 0.657
6.444GluLys: 6.444 ± 0.753
8.227GluLeu: 8.227 ± 0.817
1.782GluMet: 1.782 ± 0.403
3.153GluAsn: 3.153 ± 0.512
1.645GluPro: 1.645 ± 0.294
3.359GluGln: 3.359 ± 0.465
2.742GluArg: 2.742 ± 0.434
3.976GluSer: 3.976 ± 0.595
4.045GluThr: 4.045 ± 0.634
5.347GluVal: 5.347 ± 0.636
1.165GluTrp: 1.165 ± 0.274
3.77GluTyr: 3.77 ± 0.593
0.0GluXaa: 0.0 ± 0.0
Phe
2.879PheAla: 2.879 ± 0.388
0.274PheCys: 0.274 ± 0.165
2.811PheAsp: 2.811 ± 0.363
2.811PheGlu: 2.811 ± 0.459
0.754PhePhe: 0.754 ± 0.257
2.468PheGly: 2.468 ± 0.5
0.823PheHis: 0.823 ± 0.262
1.851PheIle: 1.851 ± 0.34
2.811PheLys: 2.811 ± 0.437
2.879PheLeu: 2.879 ± 0.464
0.754PheMet: 0.754 ± 0.213
1.92PheAsn: 1.92 ± 0.341
1.508PhePro: 1.508 ± 0.388
1.44PheGln: 1.44 ± 0.313
1.371PheArg: 1.371 ± 0.3
2.674PheSer: 2.674 ± 0.479
3.153PheThr: 3.153 ± 0.397
1.92PheVal: 1.92 ± 0.251
0.411PheTrp: 0.411 ± 0.188
1.44PheTyr: 1.44 ± 0.288
0.0PheXaa: 0.0 ± 0.0
Gly
4.593GlyAla: 4.593 ± 0.758
0.823GlyCys: 0.823 ± 0.219
4.867GlyAsp: 4.867 ± 0.455
4.25GlyGlu: 4.25 ± 0.581
3.565GlyPhe: 3.565 ± 0.619
3.976GlyGly: 3.976 ± 0.621
1.165GlyHis: 1.165 ± 0.254
5.416GlyIle: 5.416 ± 0.551
6.17GlyLys: 6.17 ± 0.686
6.376GlyLeu: 6.376 ± 0.753
1.782GlyMet: 1.782 ± 0.452
4.113GlyAsn: 4.113 ± 0.419
0.274GlyPro: 0.274 ± 0.142
1.92GlyGln: 1.92 ± 0.391
2.811GlyArg: 2.811 ± 0.448
4.045GlySer: 4.045 ± 0.604
3.016GlyThr: 3.016 ± 0.421
4.25GlyVal: 4.25 ± 0.735
1.097GlyTrp: 1.097 ± 0.291
3.976GlyTyr: 3.976 ± 0.476
0.0GlyXaa: 0.0 ± 0.0
His
1.165HisAla: 1.165 ± 0.298
0.274HisCys: 0.274 ± 0.168
0.48HisAsp: 0.48 ± 0.177
1.371HisGlu: 1.371 ± 0.333
0.891HisPhe: 0.891 ± 0.256
1.508HisGly: 1.508 ± 0.392
0.96HisHis: 0.96 ± 0.277
1.028HisIle: 1.028 ± 0.293
1.714HisLys: 1.714 ± 0.395
1.303HisLeu: 1.303 ± 0.33
0.48HisMet: 0.48 ± 0.14
0.891HisAsn: 0.891 ± 0.299
0.891HisPro: 0.891 ± 0.234
0.548HisGln: 0.548 ± 0.188
1.577HisArg: 1.577 ± 0.36
1.234HisSer: 1.234 ± 0.267
1.165HisThr: 1.165 ± 0.227
0.96HisVal: 0.96 ± 0.226
0.206HisTrp: 0.206 ± 0.095
0.823HisTyr: 0.823 ± 0.242
0.0HisXaa: 0.0 ± 0.0
Ile
4.25IleAla: 4.25 ± 0.565
0.823IleCys: 0.823 ± 0.205
4.867IleAsp: 4.867 ± 0.557
5.553IleGlu: 5.553 ± 0.541
1.371IlePhe: 1.371 ± 0.254
3.839IleGly: 3.839 ± 0.529
1.028IleHis: 1.028 ± 0.246
3.016IleIle: 3.016 ± 0.453
5.621IleLys: 5.621 ± 0.651
4.113IleLeu: 4.113 ± 0.417
1.714IleMet: 1.714 ± 0.234
4.25IleAsn: 4.25 ± 0.604
2.537IlePro: 2.537 ± 0.382
3.016IleGln: 3.016 ± 0.399
2.399IleArg: 2.399 ± 0.335
4.593IleSer: 4.593 ± 0.693
4.73IleThr: 4.73 ± 0.622
4.113IleVal: 4.113 ± 0.464
0.686IleTrp: 0.686 ± 0.238
2.331IleTyr: 2.331 ± 0.389
0.0IleXaa: 0.0 ± 0.0
Lys
6.513LysAla: 6.513 ± 0.826
0.411LysCys: 0.411 ± 0.236
4.593LysAsp: 4.593 ± 0.468
6.993LysGlu: 6.993 ± 0.905
3.222LysPhe: 3.222 ± 0.496
6.033LysGly: 6.033 ± 0.739
1.303LysHis: 1.303 ± 0.292
4.319LysIle: 4.319 ± 0.65
5.964LysLys: 5.964 ± 0.65
6.376LysLeu: 6.376 ± 0.841
2.399LysMet: 2.399 ± 0.34
3.222LysAsn: 3.222 ± 0.452
2.879LysPro: 2.879 ± 0.467
3.291LysGln: 3.291 ± 0.484
3.77LysArg: 3.77 ± 0.538
3.633LysSer: 3.633 ± 0.518
3.702LysThr: 3.702 ± 0.553
4.662LysVal: 4.662 ± 0.652
1.371LysTrp: 1.371 ± 0.429
3.359LysTyr: 3.359 ± 0.473
0.0LysXaa: 0.0 ± 0.0
Leu
5.21LeuAla: 5.21 ± 0.775
0.617LeuCys: 0.617 ± 0.241
5.621LeuAsp: 5.621 ± 0.539
6.924LeuGlu: 6.924 ± 0.726
2.468LeuPhe: 2.468 ± 0.323
5.759LeuGly: 5.759 ± 0.564
1.92LeuHis: 1.92 ± 0.347
4.936LeuIle: 4.936 ± 0.602
4.525LeuLys: 4.525 ± 0.624
5.484LeuLeu: 5.484 ± 0.569
1.782LeuMet: 1.782 ± 0.378
3.633LeuAsn: 3.633 ± 0.551
2.674LeuPro: 2.674 ± 0.398
3.908LeuGln: 3.908 ± 0.747
3.77LeuArg: 3.77 ± 0.518
5.347LeuSer: 5.347 ± 0.595
5.621LeuThr: 5.621 ± 0.711
4.525LeuVal: 4.525 ± 0.519
1.028LeuTrp: 1.028 ± 0.253
3.291LeuTyr: 3.291 ± 0.407
0.0LeuXaa: 0.0 ± 0.0
Met
2.399MetAla: 2.399 ± 0.533
0.069MetCys: 0.069 ± 0.064
1.371MetAsp: 1.371 ± 0.31
2.057MetGlu: 2.057 ± 0.361
1.234MetPhe: 1.234 ± 0.288
1.234MetGly: 1.234 ± 0.304
0.274MetHis: 0.274 ± 0.155
2.125MetIle: 2.125 ± 0.383
1.988MetLys: 1.988 ± 0.429
1.097MetLeu: 1.097 ± 0.28
0.686MetMet: 0.686 ± 0.201
1.097MetAsn: 1.097 ± 0.319
0.686MetPro: 0.686 ± 0.167
0.823MetGln: 0.823 ± 0.245
1.508MetArg: 1.508 ± 0.407
2.125MetSer: 2.125 ± 0.343
2.331MetThr: 2.331 ± 0.329
1.645MetVal: 1.645 ± 0.399
0.343MetTrp: 0.343 ± 0.167
1.028MetTyr: 1.028 ± 0.246
0.0MetXaa: 0.0 ± 0.0
Asn
2.742AsnAla: 2.742 ± 0.728
0.206AsnCys: 0.206 ± 0.111
2.948AsnAsp: 2.948 ± 0.408
3.496AsnGlu: 3.496 ± 0.397
1.645AsnPhe: 1.645 ± 0.439
4.25AsnGly: 4.25 ± 0.711
1.028AsnHis: 1.028 ± 0.305
2.674AsnIle: 2.674 ± 0.462
3.839AsnLys: 3.839 ± 0.55
3.496AsnLeu: 3.496 ± 0.51
2.194AsnMet: 2.194 ± 0.449
3.153AsnAsn: 3.153 ± 0.452
2.057AsnPro: 2.057 ± 0.248
1.851AsnGln: 1.851 ± 0.438
2.537AsnArg: 2.537 ± 0.407
3.77AsnSer: 3.77 ± 0.469
3.565AsnThr: 3.565 ± 0.631
2.879AsnVal: 2.879 ± 0.56
0.891AsnTrp: 0.891 ± 0.202
2.811AsnTyr: 2.811 ± 0.43
0.0AsnXaa: 0.0 ± 0.0
Pro
2.605ProAla: 2.605 ± 0.459
0.274ProCys: 0.274 ± 0.124
1.851ProAsp: 1.851 ± 0.341
2.948ProGlu: 2.948 ± 0.579
1.097ProPhe: 1.097 ± 0.273
0.823ProGly: 0.823 ± 0.234
0.617ProHis: 0.617 ± 0.213
1.645ProIle: 1.645 ± 0.273
2.742ProLys: 2.742 ± 0.464
2.948ProLeu: 2.948 ± 0.426
0.617ProMet: 0.617 ± 0.193
2.262ProAsn: 2.262 ± 0.324
0.823ProPro: 0.823 ± 0.222
1.028ProGln: 1.028 ± 0.256
1.577ProArg: 1.577 ± 0.359
2.057ProSer: 2.057 ± 0.432
2.605ProThr: 2.605 ± 0.354
2.057ProVal: 2.057 ± 0.355
0.274ProTrp: 0.274 ± 0.137
1.028ProTyr: 1.028 ± 0.23
0.0ProXaa: 0.0 ± 0.0
Gln
3.565GlnAla: 3.565 ± 0.538
0.274GlnCys: 0.274 ± 0.156
2.331GlnAsp: 2.331 ± 0.808
3.085GlnGlu: 3.085 ± 0.378
1.303GlnPhe: 1.303 ± 0.282
2.879GlnGly: 2.879 ± 0.505
0.48GlnHis: 0.48 ± 0.166
2.331GlnIle: 2.331 ± 0.491
2.331GlnLys: 2.331 ± 0.33
3.976GlnLeu: 3.976 ± 0.478
1.165GlnMet: 1.165 ± 0.275
1.714GlnAsn: 1.714 ± 0.34
1.234GlnPro: 1.234 ± 0.309
1.371GlnGln: 1.371 ± 0.314
1.645GlnArg: 1.645 ± 0.349
1.92GlnSer: 1.92 ± 0.336
2.057GlnThr: 2.057 ± 0.47
2.194GlnVal: 2.194 ± 0.377
0.343GlnTrp: 0.343 ± 0.185
1.44GlnTyr: 1.44 ± 0.314
0.0GlnXaa: 0.0 ± 0.0
Arg
2.331ArgAla: 2.331 ± 0.408
0.411ArgCys: 0.411 ± 0.175
2.331ArgAsp: 2.331 ± 0.457
3.359ArgGlu: 3.359 ± 0.498
1.988ArgPhe: 1.988 ± 0.326
2.399ArgGly: 2.399 ± 0.452
0.96ArgHis: 0.96 ± 0.327
4.045ArgIle: 4.045 ± 0.539
4.525ArgLys: 4.525 ± 0.652
3.839ArgLeu: 3.839 ± 0.418
1.645ArgMet: 1.645 ± 0.293
2.057ArgAsn: 2.057 ± 0.514
1.371ArgPro: 1.371 ± 0.301
2.125ArgGln: 2.125 ± 0.38
1.92ArgArg: 1.92 ± 0.342
2.674ArgSer: 2.674 ± 0.372
2.194ArgThr: 2.194 ± 0.483
2.879ArgVal: 2.879 ± 0.411
0.343ArgTrp: 0.343 ± 0.133
2.537ArgTyr: 2.537 ± 0.465
0.0ArgXaa: 0.0 ± 0.0
Ser
3.565SerAla: 3.565 ± 0.436
0.754SerCys: 0.754 ± 0.217
3.016SerAsp: 3.016 ± 0.378
4.593SerGlu: 4.593 ± 0.618
2.262SerPhe: 2.262 ± 0.41
5.896SerGly: 5.896 ± 0.731
0.686SerHis: 0.686 ± 0.18
3.565SerIle: 3.565 ± 0.546
5.621SerLys: 5.621 ± 0.604
5.416SerLeu: 5.416 ± 0.58
2.125SerMet: 2.125 ± 0.398
3.016SerAsn: 3.016 ± 0.565
1.851SerPro: 1.851 ± 0.45
3.153SerGln: 3.153 ± 0.474
3.291SerArg: 3.291 ± 0.728
4.113SerSer: 4.113 ± 0.745
3.633SerThr: 3.633 ± 0.467
3.496SerVal: 3.496 ± 0.554
1.44SerTrp: 1.44 ± 0.322
2.125SerTyr: 2.125 ± 0.34
0.0SerXaa: 0.0 ± 0.0
Thr
4.456ThrAla: 4.456 ± 0.75
0.754ThrCys: 0.754 ± 0.222
3.976ThrAsp: 3.976 ± 0.446
4.593ThrGlu: 4.593 ± 0.662
2.194ThrPhe: 2.194 ± 0.328
5.004ThrGly: 5.004 ± 0.655
1.371ThrHis: 1.371 ± 0.3
3.976ThrIle: 3.976 ± 0.568
4.113ThrLys: 4.113 ± 0.658
4.045ThrLeu: 4.045 ± 0.494
0.548ThrMet: 0.548 ± 0.232
2.331ThrAsn: 2.331 ± 0.409
2.879ThrPro: 2.879 ± 0.491
2.399ThrGln: 2.399 ± 0.492
3.291ThrArg: 3.291 ± 0.54
3.908ThrSer: 3.908 ± 0.545
3.702ThrThr: 3.702 ± 0.68
4.387ThrVal: 4.387 ± 0.654
0.754ThrTrp: 0.754 ± 0.249
2.537ThrTyr: 2.537 ± 0.464
0.0ThrXaa: 0.0 ± 0.0
Val
5.073ValAla: 5.073 ± 0.651
0.686ValCys: 0.686 ± 0.208
4.799ValAsp: 4.799 ± 0.52
4.936ValGlu: 4.936 ± 0.645
1.714ValPhe: 1.714 ± 0.371
4.25ValGly: 4.25 ± 0.493
1.097ValHis: 1.097 ± 0.237
3.77ValIle: 3.77 ± 0.436
3.908ValLys: 3.908 ± 0.512
4.387ValLeu: 4.387 ± 0.6
1.303ValMet: 1.303 ± 0.267
3.839ValAsn: 3.839 ± 0.644
1.92ValPro: 1.92 ± 0.297
1.782ValGln: 1.782 ± 0.372
2.399ValArg: 2.399 ± 0.411
3.976ValSer: 3.976 ± 0.52
4.25ValThr: 4.25 ± 0.478
3.222ValVal: 3.222 ± 0.456
0.48ValTrp: 0.48 ± 0.196
2.742ValTyr: 2.742 ± 0.449
0.0ValXaa: 0.0 ± 0.0
Trp
0.891TrpAla: 0.891 ± 0.251
0.343TrpCys: 0.343 ± 0.145
0.411TrpAsp: 0.411 ± 0.178
1.028TrpGlu: 1.028 ± 0.409
0.343TrpPhe: 0.343 ± 0.141
0.548TrpGly: 0.548 ± 0.205
0.411TrpHis: 0.411 ± 0.155
1.028TrpIle: 1.028 ± 0.252
0.686TrpLys: 0.686 ± 0.184
0.891TrpLeu: 0.891 ± 0.284
0.48TrpMet: 0.48 ± 0.174
0.96TrpAsn: 0.96 ± 0.278
0.069TrpPro: 0.069 ± 0.067
0.343TrpGln: 0.343 ± 0.121
0.548TrpArg: 0.548 ± 0.19
1.577TrpSer: 1.577 ± 0.459
0.96TrpThr: 0.96 ± 0.274
0.891TrpVal: 0.891 ± 0.224
0.274TrpTrp: 0.274 ± 0.136
0.686TrpTyr: 0.686 ± 0.258
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.537TyrAla: 2.537 ± 0.378
0.411TyrCys: 0.411 ± 0.151
2.742TyrAsp: 2.742 ± 0.479
3.359TyrGlu: 3.359 ± 0.515
2.125TyrPhe: 2.125 ± 0.396
3.565TyrGly: 3.565 ± 0.492
0.96TyrHis: 0.96 ± 0.267
2.468TyrIle: 2.468 ± 0.383
3.428TyrLys: 3.428 ± 0.556
3.291TyrLeu: 3.291 ± 0.622
1.234TyrMet: 1.234 ± 0.241
2.262TyrAsn: 2.262 ± 0.45
1.508TyrPro: 1.508 ± 0.293
1.577TyrGln: 1.577 ± 0.362
1.782TyrArg: 1.782 ± 0.386
3.291TyrSer: 3.291 ± 0.464
2.537TyrThr: 2.537 ± 0.465
2.468TyrVal: 2.468 ± 0.42
0.343TyrTrp: 0.343 ± 0.139
2.468TyrTyr: 2.468 ± 0.487
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 75 proteins (14588 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski