Meiothermus silvanus (strain ATCC 700542 / DSM 9946 / VI-R2) (Thermus silvanus)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae; Meiothermus; Meiothermus silvanus

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3383 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D7BJU0|D7BJU0_MEISD Uncharacterized protein OS=Meiothermus silvanus (strain ATCC 700542 / DSM 9946 / VI-R2) OX=526227 GN=Mesil_3660 PE=4 SV=1
MM1 pKa = 7.92EE2 pKa = 5.49ILPIFFLTLAFSLVYY17 pKa = 10.62VILNAIFGFSDD28 pKa = 3.5DD29 pKa = 4.44NYY31 pKa = 9.4VVYY34 pKa = 10.8YY35 pKa = 10.25DD36 pKa = 3.52RR37 pKa = 11.84EE38 pKa = 4.1VRR40 pKa = 11.84YY41 pKa = 9.89EE42 pKa = 3.94EE43 pKa = 4.51PPFF46 pKa = 5.1

Molecular weight: 5.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D7BGR9|D7BGR9_MEISD Serine/threonine protein kinase OS=Meiothermus silvanus (strain ATCC 700542 / DSM 9946 / VI-R2) OX=526227 GN=Mesil_0128 PE=4 SV=1
MM1 pKa = 7.57LRR3 pKa = 11.84LQGPWAGLAFGLAAAMGLLLVVWLLATLWILALIGGLIGLAALGWQRR50 pKa = 11.84LRR52 pKa = 11.84LRR54 pKa = 11.84LGGGWRR60 pKa = 11.84RR61 pKa = 11.84TRR63 pKa = 11.84PKK65 pKa = 10.31PRR67 pKa = 11.84LQRR70 pKa = 11.84LPPPWRR76 pKa = 4.53

Molecular weight: 8.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3383

0

3383

1043606

30

2780

308.5

33.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.877 ± 0.055

0.549 ± 0.012

4.03 ± 0.027

6.984 ± 0.05

3.57 ± 0.025

8.7 ± 0.043

1.96 ± 0.022

4.052 ± 0.029

3.52 ± 0.033

12.519 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.818 ± 0.018

2.351 ± 0.021

5.948 ± 0.038

3.917 ± 0.024

7.434 ± 0.04

4.977 ± 0.031

4.683 ± 0.027

7.538 ± 0.034

1.627 ± 0.021

2.949 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski