Streptomyces sp. CB01201

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7508 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2M9J2R3|A0A2M9J2R3_9ACTN Uncharacterized protein OS=Streptomyces sp. CB01201 OX=2020324 GN=CG740_21065 PE=4 SV=1
MM1 pKa = 7.55NEE3 pKa = 4.07ALEE6 pKa = 4.13VWIDD10 pKa = 3.41QDD12 pKa = 3.99LCTGDD17 pKa = 4.65GICVQYY23 pKa = 10.96APEE26 pKa = 4.18VFEE29 pKa = 5.9LDD31 pKa = 3.05IDD33 pKa = 3.63GLAYY37 pKa = 10.42VKK39 pKa = 10.8SADD42 pKa = 4.59DD43 pKa = 4.34EE44 pKa = 4.66LLQTPRR50 pKa = 11.84ATTPVPLPLLQDD62 pKa = 3.66VVDD65 pKa = 4.35SAKK68 pKa = 10.25EE69 pKa = 3.92CPGDD73 pKa = 4.18CIHH76 pKa = 6.28VRR78 pKa = 11.84RR79 pKa = 11.84VSDD82 pKa = 3.47SVEE85 pKa = 4.14VYY87 pKa = 10.93GPDD90 pKa = 3.69AEE92 pKa = 4.31

Molecular weight: 10.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2M9J6H7|A0A2M9J6H7_9ACTN Uncharacterized protein OS=Streptomyces sp. CB01201 OX=2020324 GN=CG740_15655 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.51GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7508

0

7508

2436049

29

7016

324.5

34.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.795 ± 0.04

0.806 ± 0.008

5.834 ± 0.024

5.481 ± 0.031

2.762 ± 0.015

9.509 ± 0.026

2.32 ± 0.014

3.233 ± 0.02

2.255 ± 0.026

10.357 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.751 ± 0.012

1.842 ± 0.016

6.151 ± 0.026

2.877 ± 0.018

7.857 ± 0.03

5.233 ± 0.023

6.047 ± 0.026

8.28 ± 0.027

1.516 ± 0.012

2.095 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski