Amino acid dipepetide frequency for Streptomyces sp. CB01201

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.807AlaAla: 21.807 ± 0.13	1.07AlaCys: 1.07 ± 0.022	8.173AlaAsp: 8.173 ± 0.066	8.719AlaGlu: 8.719 ± 0.096	3.627AlaPhe: 3.627 ± 0.036	13.206AlaGly: 13.206 ± 0.087	2.979AlaHis: 2.979 ± 0.039	3.688AlaIle: 3.688 ± 0.043	3.175AlaLys: 3.175 ± 0.047	14.855AlaLeu: 14.855 ± 0.107	2.592AlaMet: 2.592 ± 0.034	2.099AlaAsn: 2.099 ± 0.036	7.584AlaPro: 7.584 ± 0.07	3.995AlaGln: 3.995 ± 0.05	10.174AlaArg: 10.174 ± 0.079	6.284AlaSer: 6.284 ± 0.055	7.096AlaThr: 7.096 ± 0.059	12.165AlaVal: 12.165 ± 0.082	1.864AlaTrp: 1.864 ± 0.029	2.797AlaTyr: 2.797 ± 0.038	0.0AlaXaa: 0.0 ± 0.0
Cys	1.119CysAla: 1.119 ± 0.022	0.089CysCys: 0.089 ± 0.006	0.461CysAsp: 0.461 ± 0.014	0.417CysGlu: 0.417 ± 0.017	0.222CysPhe: 0.222 ± 0.009	0.994CysGly: 0.994 ± 0.024	0.197CysHis: 0.197 ± 0.01	0.187CysIle: 0.187 ± 0.008	0.122CysLys: 0.122 ± 0.007	0.744CysLeu: 0.744 ± 0.018	0.123CysMet: 0.123 ± 0.007	0.14CysAsn: 0.14 ± 0.008	0.507CysPro: 0.507 ± 0.016	0.179CysGln: 0.179 ± 0.008	0.62CysArg: 0.62 ± 0.017	0.445CysSer: 0.445 ± 0.014	0.541CysThr: 0.541 ± 0.015	0.668CysVal: 0.668 ± 0.018	0.131CysTrp: 0.131 ± 0.007	0.157CysTyr: 0.157 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	7.663AspAla: 7.663 ± 0.062	0.427AspCys: 0.427 ± 0.015	3.378AspAsp: 3.378 ± 0.04	3.697AspGlu: 3.697 ± 0.046	1.624AspPhe: 1.624 ± 0.024	6.07AspGly: 6.07 ± 0.059	1.426AspHis: 1.426 ± 0.023	1.947AspIle: 1.947 ± 0.027	1.303AspLys: 1.303 ± 0.028	6.066AspLeu: 6.066 ± 0.066	0.803AspMet: 0.803 ± 0.017	0.988AspAsn: 0.988 ± 0.021	4.414AspPro: 4.414 ± 0.049	1.644AspGln: 1.644 ± 0.028	4.706AspArg: 4.706 ± 0.057	2.55AspSer: 2.55 ± 0.033	3.062AspThr: 3.062 ± 0.036	4.445AspVal: 4.445 ± 0.047	1.02AspTrp: 1.02 ± 0.021	1.105AspTyr: 1.105 ± 0.023	0.0AspXaa: 0.0 ± 0.0
Glu	7.28GluAla: 7.28 ± 0.087	0.357GluCys: 0.357 ± 0.012	2.615GluAsp: 2.615 ± 0.041	3.167GluGlu: 3.167 ± 0.046	1.436GluPhe: 1.436 ± 0.025	4.191GluGly: 4.191 ± 0.043	1.482GluHis: 1.482 ± 0.025	2.156GluIle: 2.156 ± 0.033	1.328GluLys: 1.328 ± 0.031	6.855GluLeu: 6.855 ± 0.073	0.843GluMet: 0.843 ± 0.018	0.96GluAsn: 0.96 ± 0.022	3.256GluPro: 3.256 ± 0.043	2.25GluGln: 2.25 ± 0.034	5.372GluArg: 5.372 ± 0.065	2.631GluSer: 2.631 ± 0.036	2.605GluThr: 2.605 ± 0.037	4.256GluVal: 4.256 ± 0.053	0.709GluTrp: 0.709 ± 0.016	1.055GluTyr: 1.055 ± 0.023	0.0GluXaa: 0.0 ± 0.0
Phe	3.867PheAla: 3.867 ± 0.037	0.287PheCys: 0.287 ± 0.009	1.953PheAsp: 1.953 ± 0.029	1.426PheGlu: 1.426 ± 0.027	0.898PhePhe: 0.898 ± 0.021	3.057PheGly: 3.057 ± 0.037	0.628PheHis: 0.628 ± 0.017	0.771PheIle: 0.771 ± 0.018	0.552PheLys: 0.552 ± 0.018	2.547PheLeu: 2.547 ± 0.036	0.413PheMet: 0.413 ± 0.011	0.597PheAsn: 0.597 ± 0.015	1.41PhePro: 1.41 ± 0.025	0.716PheGln: 0.716 ± 0.017	1.764PheArg: 1.764 ± 0.027	1.469PheSer: 1.469 ± 0.029	2.062PheThr: 2.062 ± 0.033	2.218PheVal: 2.218 ± 0.031	0.408PheTrp: 0.408 ± 0.013	0.579PheTyr: 0.579 ± 0.016	0.0PheXaa: 0.0 ± 0.0
Gly	11.505GlyAla: 11.505 ± 0.075	0.9GlyCys: 0.9 ± 0.019	5.027GlyAsp: 5.027 ± 0.05	4.915GlyGlu: 4.915 ± 0.05	2.906GlyPhe: 2.906 ± 0.04	8.848GlyGly: 8.848 ± 0.087	2.363GlyHis: 2.363 ± 0.033	3.527GlyIle: 3.527 ± 0.039	2.495GlyLys: 2.495 ± 0.04	9.513GlyLeu: 9.513 ± 0.071	1.972GlyMet: 1.972 ± 0.027	1.797GlyAsn: 1.797 ± 0.035	5.426GlyPro: 5.426 ± 0.064	2.782GlyGln: 2.782 ± 0.046	7.772GlyArg: 7.772 ± 0.058	5.738GlySer: 5.738 ± 0.06	6.214GlyThr: 6.214 ± 0.058	7.334GlyVal: 7.334 ± 0.06	1.766GlyTrp: 1.766 ± 0.032	2.286GlyTyr: 2.286 ± 0.032	0.0GlyXaa: 0.0 ± 0.0
His	2.706HisAla: 2.706 ± 0.034	0.219HisCys: 0.219 ± 0.009	1.333HisAsp: 1.333 ± 0.023	1.176HisGlu: 1.176 ± 0.022	0.67HisPhe: 0.67 ± 0.016	2.444HisGly: 2.444 ± 0.035	0.723HisHis: 0.723 ± 0.022	0.735HisIle: 0.735 ± 0.018	0.368HisLys: 0.368 ± 0.012	2.43HisLeu: 2.43 ± 0.033	0.355HisMet: 0.355 ± 0.012	0.404HisAsn: 0.404 ± 0.013	1.844HisPro: 1.844 ± 0.03	0.752HisGln: 0.752 ± 0.019	2.053HisArg: 2.053 ± 0.031	1.082HisSer: 1.082 ± 0.022	1.42HisThr: 1.42 ± 0.026	1.622HisVal: 1.622 ± 0.024	0.384HisTrp: 0.384 ± 0.013	0.486HisTyr: 0.486 ± 0.014	0.0HisXaa: 0.0 ± 0.0
Ile	5.004IleAla: 5.004 ± 0.05	0.309IleCys: 0.309 ± 0.012	2.261IleAsp: 2.261 ± 0.031	2.084IleGlu: 2.084 ± 0.031	0.704IlePhe: 0.704 ± 0.019	3.724IleGly: 3.724 ± 0.048	0.627IleHis: 0.627 ± 0.015	0.855IleIle: 0.855 ± 0.021	0.768IleLys: 0.768 ± 0.02	2.454IleLeu: 2.454 ± 0.032	0.431IleMet: 0.431 ± 0.014	0.75IleAsn: 0.75 ± 0.018	1.796IlePro: 1.796 ± 0.029	0.783IleGln: 0.783 ± 0.018	2.149IleArg: 2.149 ± 0.031	1.706IleSer: 1.706 ± 0.027	2.24IleThr: 2.24 ± 0.034	2.75IleVal: 2.75 ± 0.037	0.404IleTrp: 0.404 ± 0.013	0.528IleTyr: 0.528 ± 0.014	0.0IleXaa: 0.0 ± 0.0
Lys	3.106LysAla: 3.106 ± 0.051	0.128LysCys: 0.128 ± 0.008	1.426LysAsp: 1.426 ± 0.033	1.19LysGlu: 1.19 ± 0.024	0.498LysPhe: 0.498 ± 0.017	1.919LysGly: 1.919 ± 0.033	0.454LysHis: 0.454 ± 0.015	0.89LysIle: 0.89 ± 0.02	0.876LysLys: 0.876 ± 0.028	2.099LysLeu: 2.099 ± 0.035	0.417LysMet: 0.417 ± 0.015	0.577LysAsn: 0.577 ± 0.018	1.447LysPro: 1.447 ± 0.03	0.754LysGln: 0.754 ± 0.018	1.444LysArg: 1.444 ± 0.026	1.278LysSer: 1.278 ± 0.025	1.335LysThr: 1.335 ± 0.027	1.947LysVal: 1.947 ± 0.031	0.298LysTrp: 0.298 ± 0.011	0.469LysTyr: 0.469 ± 0.014	0.0LysXaa: 0.0 ± 0.0
Leu	15.476LeuAla: 15.476 ± 0.099	0.893LeuCys: 0.893 ± 0.02	6.78LeuAsp: 6.78 ± 0.069	4.443LeuGlu: 4.443 ± 0.054	2.716LeuPhe: 2.716 ± 0.04	9.426LeuGly: 9.426 ± 0.072	2.337LeuHis: 2.337 ± 0.034	3.444LeuIle: 3.444 ± 0.045	2.169LeuLys: 2.169 ± 0.033	11.182LeuLeu: 11.182 ± 0.095	1.671LeuMet: 1.671 ± 0.029	1.82LeuAsn: 1.82 ± 0.028	6.435LeuPro: 6.435 ± 0.061	2.216LeuGln: 2.216 ± 0.03	8.502LeuArg: 8.502 ± 0.071	5.41LeuSer: 5.41 ± 0.052	6.746LeuThr: 6.746 ± 0.063	8.688LeuVal: 8.688 ± 0.066	1.341LeuTrp: 1.341 ± 0.026	1.871LeuTyr: 1.871 ± 0.032	0.0LeuXaa: 0.0 ± 0.0
Met	2.341MetAla: 2.341 ± 0.033	0.148MetCys: 0.148 ± 0.007	0.888MetAsp: 0.888 ± 0.019	0.743MetGlu: 0.743 ± 0.017	0.454MetPhe: 0.454 ± 0.014	1.331MetGly: 1.331 ± 0.023	0.35MetHis: 0.35 ± 0.014	0.647MetIle: 0.647 ± 0.018	0.438MetLys: 0.438 ± 0.012	1.699MetLeu: 1.699 ± 0.031	0.304MetMet: 0.304 ± 0.012	0.465MetAsn: 0.465 ± 0.016	1.161MetPro: 1.161 ± 0.021	0.441MetGln: 0.441 ± 0.015	1.431MetArg: 1.431 ± 0.025	1.36MetSer: 1.36 ± 0.024	1.465MetThr: 1.465 ± 0.026	1.285MetVal: 1.285 ± 0.025	0.222MetTrp: 0.222 ± 0.01	0.333MetTyr: 0.333 ± 0.011	0.0MetXaa: 0.0 ± 0.0
Asn	2.332AsnAla: 2.332 ± 0.035	0.171AsnCys: 0.171 ± 0.009	0.953AsnAsp: 0.953 ± 0.021	0.814AsnGlu: 0.814 ± 0.018	0.506AsnPhe: 0.506 ± 0.016	1.975AsnGly: 1.975 ± 0.036	0.406AsnHis: 0.406 ± 0.013	0.677AsnIle: 0.677 ± 0.017	0.465AsnLys: 0.465 ± 0.013	1.702AsnLeu: 1.702 ± 0.031	0.344AsnMet: 0.344 ± 0.012	0.463AsnAsn: 0.463 ± 0.018	1.374AsnPro: 1.374 ± 0.027	0.549AsnGln: 0.549 ± 0.016	1.284AsnArg: 1.284 ± 0.022	1.057AsnSer: 1.057 ± 0.025	1.232AsnThr: 1.232 ± 0.028	1.371AsnVal: 1.371 ± 0.027	0.319AsnTrp: 0.319 ± 0.013	0.43AsnTyr: 0.43 ± 0.015	0.0AsnXaa: 0.0 ± 0.0
Pro	9.037ProAla: 9.037 ± 0.086	0.356ProCys: 0.356 ± 0.012	4.312ProAsp: 4.312 ± 0.048	4.157ProGlu: 4.157 ± 0.051	1.527ProPhe: 1.527 ± 0.024	6.868ProGly: 6.868 ± 0.06	1.406ProHis: 1.406 ± 0.026	1.317ProIle: 1.317 ± 0.024	1.255ProLys: 1.255 ± 0.026	5.499ProLeu: 5.499 ± 0.054	1.021ProMet: 1.021 ± 0.021	0.996ProAsn: 0.996 ± 0.021	3.547ProPro: 3.547 ± 0.056	1.912ProGln: 1.912 ± 0.036	3.984ProArg: 3.984 ± 0.044	3.355ProSer: 3.355 ± 0.05	3.165ProThr: 3.165 ± 0.036	5.52ProVal: 5.52 ± 0.053	0.885ProTrp: 0.885 ± 0.021	1.39ProTyr: 1.39 ± 0.023	0.0ProXaa: 0.0 ± 0.0
Gln	3.79GlnAla: 3.79 ± 0.046	0.191GlnCys: 0.191 ± 0.009	1.447GlnAsp: 1.447 ± 0.024	1.456GlnGlu: 1.456 ± 0.027	0.724GlnPhe: 0.724 ± 0.017	2.436GlnGly: 2.436 ± 0.037	0.752GlnHis: 0.752 ± 0.022	1.104GlnIle: 1.104 ± 0.023	0.655GlnLys: 0.655 ± 0.019	3.309GlnLeu: 3.309 ± 0.041	0.524GlnMet: 0.524 ± 0.015	0.544GlnAsn: 0.544 ± 0.018	1.777GlnPro: 1.777 ± 0.038	1.332GlnGln: 1.332 ± 0.033	2.399GlnArg: 2.399 ± 0.038	1.427GlnSer: 1.427 ± 0.025	1.323GlnThr: 1.323 ± 0.022	2.431GlnVal: 2.431 ± 0.037	0.475GlnTrp: 0.475 ± 0.013	0.671GlnTyr: 0.671 ± 0.019	0.0GlnXaa: 0.0 ± 0.0
Arg	9.878ArgAla: 9.878 ± 0.075	0.598ArgCys: 0.598 ± 0.015	4.13ArgAsp: 4.13 ± 0.048	4.502ArgGlu: 4.502 ± 0.047	2.321ArgPhe: 2.321 ± 0.028	5.853ArgGly: 5.853 ± 0.054	2.074ArgHis: 2.074 ± 0.034	3.273ArgIle: 3.273 ± 0.035	1.608ArgLys: 1.608 ± 0.031	8.585ArgLeu: 8.585 ± 0.08	1.683ArgMet: 1.683 ± 0.027	1.354ArgAsn: 1.354 ± 0.025	4.99ArgPro: 4.99 ± 0.056	2.279ArgGln: 2.279 ± 0.032	7.41ArgArg: 7.41 ± 0.073	4.074ArgSer: 4.074 ± 0.046	5.416ArgThr: 5.416 ± 0.052	5.523ArgVal: 5.523 ± 0.05	1.327ArgTrp: 1.327 ± 0.028	1.696ArgTyr: 1.696 ± 0.029	0.0ArgXaa: 0.0 ± 0.0
Ser	7.18SerAla: 7.18 ± 0.055	0.436SerCys: 0.436 ± 0.013	2.713SerAsp: 2.713 ± 0.036	2.377SerGlu: 2.377 ± 0.032	1.584SerPhe: 1.584 ± 0.026	6.229SerGly: 6.229 ± 0.068	1.052SerHis: 1.052 ± 0.021	1.544SerIle: 1.544 ± 0.028	1.114SerLys: 1.114 ± 0.027	4.985SerLeu: 4.985 ± 0.056	1.141SerMet: 1.141 ± 0.018	0.908SerAsn: 0.908 ± 0.021	3.296SerPro: 3.296 ± 0.044	1.343SerGln: 1.343 ± 0.025	3.664SerArg: 3.664 ± 0.041	2.974SerSer: 2.974 ± 0.043	3.171SerThr: 3.171 ± 0.042	4.367SerVal: 4.367 ± 0.046	0.947SerTrp: 0.947 ± 0.021	1.303SerTyr: 1.303 ± 0.027	0.0SerXaa: 0.0 ± 0.0
Thr	8.834ThrAla: 8.834 ± 0.071	0.403ThrCys: 0.403 ± 0.012	3.438ThrAsp: 3.438 ± 0.041	3.089ThrGlu: 3.089 ± 0.036	1.637ThrPhe: 1.637 ± 0.031	6.724ThrGly: 6.724 ± 0.07	1.176ThrHis: 1.176 ± 0.021	1.706ThrIle: 1.706 ± 0.033	1.221ThrLys: 1.221 ± 0.022	5.716ThrLeu: 5.716 ± 0.056	0.92ThrMet: 0.92 ± 0.018	1.029ThrAsn: 1.029 ± 0.021	4.147ThrPro: 4.147 ± 0.051	1.401ThrGln: 1.401 ± 0.024	3.756ThrArg: 3.756 ± 0.042	3.266ThrSer: 3.266 ± 0.049	3.809ThrThr: 3.809 ± 0.06	5.92ThrVal: 5.92 ± 0.067	0.876ThrTrp: 0.876 ± 0.021	1.403ThrTyr: 1.403 ± 0.033	0.0ThrXaa: 0.0 ± 0.0
Val	10.523ValAla: 10.523 ± 0.08	0.723ValCys: 0.723 ± 0.018	4.791ValAsp: 4.791 ± 0.047	4.529ValGlu: 4.529 ± 0.052	2.394ValPhe: 2.394 ± 0.037	6.389ValGly: 6.389 ± 0.058	1.991ValHis: 1.991 ± 0.028	2.821ValIle: 2.821 ± 0.038	1.821ValLys: 1.821 ± 0.031	9.41ValLeu: 9.41 ± 0.071	1.41ValMet: 1.41 ± 0.025	1.668ValAsn: 1.668 ± 0.029	5.224ValPro: 5.224 ± 0.053	2.144ValGln: 2.144 ± 0.032	7.006ValArg: 7.006 ± 0.062	4.265ValSer: 4.265 ± 0.043	5.354ValThr: 5.354 ± 0.05	7.698ValVal: 7.698 ± 0.072	1.066ValTrp: 1.066 ± 0.024	1.574ValTyr: 1.574 ± 0.027	0.0ValXaa: 0.0 ± 0.0
Trp	1.696TrpAla: 1.696 ± 0.026	0.163TrpCys: 0.163 ± 0.008	0.799TrpAsp: 0.799 ± 0.021	0.676TrpGlu: 0.676 ± 0.018	0.504TrpPhe: 0.504 ± 0.015	1.056TrpGly: 1.056 ± 0.023	0.391TrpHis: 0.391 ± 0.012	0.548TrpIle: 0.548 ± 0.016	0.387TrpLys: 0.387 ± 0.013	1.794TrpLeu: 1.794 ± 0.029	0.3TrpMet: 0.3 ± 0.012	0.435TrpAsn: 0.435 ± 0.014	0.803TrpPro: 0.803 ± 0.021	0.655TrpGln: 0.655 ± 0.02	1.284TrpArg: 1.284 ± 0.026	0.964TrpSer: 0.964 ± 0.022	1.014TrpThr: 1.014 ± 0.019	0.965TrpVal: 0.965 ± 0.022	0.358TrpTrp: 0.358 ± 0.013	0.371TrpTyr: 0.371 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.806TyrAla: 2.806 ± 0.034	0.196TyrCys: 0.196 ± 0.009	1.461TyrAsp: 1.461 ± 0.036	1.228TyrGlu: 1.228 ± 0.022	0.673TyrPhe: 0.673 ± 0.016	2.346TyrGly: 2.346 ± 0.035	0.39TyrHis: 0.39 ± 0.012	0.486TyrIle: 0.486 ± 0.015	0.431TyrLys: 0.431 ± 0.015	2.125TyrLeu: 2.125 ± 0.027	0.241TyrMet: 0.241 ± 0.01	0.428TyrAsn: 0.428 ± 0.016	1.072TyrPro: 1.072 ± 0.022	0.641TyrGln: 0.641 ± 0.015	1.797TyrArg: 1.797 ± 0.029	0.994TyrSer: 0.994 ± 0.024	1.203TyrThr: 1.203 ± 0.028	1.628TyrVal: 1.628 ± 0.025	0.36TyrTrp: 0.36 ± 0.012	0.446TyrTyr: 0.446 ± 0.017	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 7508 proteins (2436049 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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