Sphingosinicella humi

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingosinicellaceae; Sphingosinicella

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2892 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U2J0Q6|A0A2U2J0Q6_9SPHN LuxR family transcriptional regulator OS=Sphingosinicella humi OX=2068657 GN=DF286_02795 PE=4 SV=1
MM1 pKa = 7.07KK2 pKa = 9.68TVSLLPFIALSFVAAPAAAQLPDD25 pKa = 3.7GGITIYY31 pKa = 11.23GEE33 pKa = 4.0AKK35 pKa = 9.91AVSDD39 pKa = 3.37YY40 pKa = 10.54RR41 pKa = 11.84YY42 pKa = 10.24RR43 pKa = 11.84GISRR47 pKa = 11.84SDD49 pKa = 3.35EE50 pKa = 4.41DD51 pKa = 3.81PALQANVTVQGDD63 pKa = 3.54SGFYY67 pKa = 9.92AGAWGSTLGGIDD79 pKa = 4.74DD80 pKa = 4.04VTEE83 pKa = 4.06GDD85 pKa = 3.45IGDD88 pKa = 4.11VEE90 pKa = 4.77LDD92 pKa = 3.92LYY94 pKa = 11.23AGYY97 pKa = 8.18GTSLGLGTSVDD108 pKa = 3.65AGVLYY113 pKa = 10.61YY114 pKa = 8.91YY115 pKa = 10.75FPDD118 pKa = 4.56GEE120 pKa = 4.48GKK122 pKa = 8.41TDD124 pKa = 3.43YY125 pKa = 10.75FEE127 pKa = 5.54PYY129 pKa = 10.82ASISQQLGPVQATAGAKK146 pKa = 8.94YY147 pKa = 10.36AWDD150 pKa = 3.47QAALGGEE157 pKa = 3.98DD158 pKa = 4.07LLYY161 pKa = 11.23LFGEE165 pKa = 4.68VEE167 pKa = 4.23AGIPLTPFTLTAGGGRR183 pKa = 11.84QSAGAFGDD191 pKa = 3.46YY192 pKa = 10.13WNWHH196 pKa = 6.11LGAEE200 pKa = 4.25ATMGPLNAGVRR211 pKa = 11.84YY212 pKa = 10.25VDD214 pKa = 3.23TDD216 pKa = 4.02LPAEE220 pKa = 4.54LPNVDD225 pKa = 3.62AGLVFSVGVKK235 pKa = 8.77FF236 pKa = 4.59

Molecular weight: 24.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U2J4U0|A0A2U2J4U0_9SPHN Formamidopyrimidine-DNA glycosylase OS=Sphingosinicella humi OX=2068657 GN=mutM PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 9.84LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.51GFRR19 pKa = 11.84ARR21 pKa = 11.84KK22 pKa = 7.81ATVGGRR28 pKa = 11.84KK29 pKa = 9.05VLAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 6.76TLSAA44 pKa = 4.22

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2892

0

2892

902573

35

2578

312.1

33.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.017 ± 0.068

0.768 ± 0.014

5.847 ± 0.035

6.367 ± 0.045

3.492 ± 0.03

8.847 ± 0.066

1.948 ± 0.021

4.976 ± 0.031

2.983 ± 0.038

10.123 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.339 ± 0.022

2.356 ± 0.033

5.338 ± 0.038

2.954 ± 0.028

7.657 ± 0.05

5.212 ± 0.039

4.989 ± 0.039

7.208 ± 0.04

1.396 ± 0.021

2.181 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski