Bisgaard taxon 44 str. 111
Average proteome isoelectric point is 6.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1591 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3A1YA77|A0A3A1YA77_9PAST Uncharacterized protein OS=Bisgaard taxon 44 str. 111 OX=2028575 GN=CKF58_06900 PE=4 SV=1
MM1 pKa = 7.39 SLIGIFYY8 pKa = 10.71 GSDD11 pKa = 2.9 TGNTEE16 pKa = 3.56 NLAFMLSNKK25 pKa = 9.35 FGKK28 pKa = 10.12 EE29 pKa = 3.57 LTDD32 pKa = 3.36 VYY34 pKa = 11.38 DD35 pKa = 3.77 VAKK38 pKa = 9.32 CTQADD43 pKa = 3.79 VEE45 pKa = 4.75 KK46 pKa = 11.42 YY47 pKa = 10.68 NILLLGIPTWYY58 pKa = 10.36 YY59 pKa = 11.6 GEE61 pKa = 4.91 AQADD65 pKa = 3.72 WEE67 pKa = 4.85 DD68 pKa = 3.92 FFPTLRR74 pKa = 11.84 QVDD77 pKa = 4.12 FNEE80 pKa = 3.78 RR81 pKa = 11.84 VVAIFGCGDD90 pKa = 3.19 QEE92 pKa = 5.04 DD93 pKa = 4.44 YY94 pKa = 11.9 ADD96 pKa = 4.07 YY97 pKa = 10.88 FCDD100 pKa = 4.42 AMGEE104 pKa = 3.93 IANIVEE110 pKa = 4.24 QNGGVVIGYY119 pKa = 6.68 TSKK122 pKa = 10.78 EE123 pKa = 4.14 GYY125 pKa = 8.73 TFTEE129 pKa = 4.25 SKK131 pKa = 11.14 ALVDD135 pKa = 3.81 EE136 pKa = 4.35 NTLVGLCIDD145 pKa = 4.32 EE146 pKa = 4.82 DD147 pKa = 3.9 RR148 pKa = 11.84 QPEE151 pKa = 4.31 LTEE154 pKa = 3.7 QRR156 pKa = 11.84 VDD158 pKa = 2.7 AWVAQLKK165 pKa = 10.56 ADD167 pKa = 4.48 LGII170 pKa = 4.39
Molecular weight: 19.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.727
IPC2_protein 3.884
IPC_protein 3.859
Toseland 3.656
ProMoST 3.999
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.745
Rodwell 3.681
Grimsley 3.567
Solomon 3.821
Lehninger 3.783
Nozaki 3.948
DTASelect 4.151
Thurlkill 3.694
EMBOSS 3.757
Sillero 3.973
Patrickios 1.074
IPC_peptide 3.821
IPC2_peptide 3.948
IPC2.peptide.svr19 3.858
Protein with the highest isoelectric point:
>tr|A0A3A1YCA9|A0A3A1YCA9_9PAST SSD domain-containing protein OS=Bisgaard taxon 44 str. 111 OX=2028575 GN=CKF58_07635 PE=4 SV=1
MM1 pKa = 7.29 FKK3 pKa = 10.65 SIALLLIRR11 pKa = 11.84 LYY13 pKa = 9.37 QTAISPLLGSGKK25 pKa = 9.61 CRR27 pKa = 11.84 YY28 pKa = 9.75 HH29 pKa = 5.83 PTCSQYY35 pKa = 11.47 GKK37 pKa = 10.54 LAIQQRR43 pKa = 11.84 GIIIGSLLTFWRR55 pKa = 11.84 VIRR58 pKa = 11.84 CHH60 pKa = 6.16 PLCIGGIDD68 pKa = 3.73 PVPSRR73 pKa = 11.84 VNNKK77 pKa = 8.79 KK78 pKa = 10.09 VKK80 pKa = 10.06 KK81 pKa = 10.26
Molecular weight: 9.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.325
IPC2_protein 9.78
IPC_protein 10.145
Toseland 10.599
ProMoST 10.262
Dawson 10.701
Bjellqvist 10.379
Wikipedia 10.862
Rodwell 11.184
Grimsley 10.76
Solomon 10.76
Lehninger 10.73
Nozaki 10.613
DTASelect 10.35
Thurlkill 10.599
EMBOSS 10.979
Sillero 10.628
Patrickios 10.95
IPC_peptide 10.76
IPC2_peptide 9.516
IPC2.peptide.svr19 8.488
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1591
0
1591
636759
18
6468
400.2
44.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.735 ± 0.082
0.848 ± 0.018
5.146 ± 0.058
5.675 ± 0.07
4.32 ± 0.053
5.488 ± 0.082
1.867 ± 0.028
6.256 ± 0.074
6.083 ± 0.063
10.274 ± 0.084
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.761 ± 0.027
6.067 ± 0.095
3.538 ± 0.046
5.397 ± 0.105
3.727 ± 0.052
6.717 ± 0.073
6.364 ± 0.097
6.814 ± 0.057
0.837 ± 0.022
4.086 ± 0.049
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here