Desulfofustis sp. PB-SRB1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfobacterales; Desulfobulbaceae; Desulfofustis; unclassified Desulfofustis

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3578 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V4J9P9|V4J9P9_9DELT Methionine aminopeptidase OS=Desulfofustis sp. PB-SRB1 OX=1385624 GN=map PE=3 SV=1
MM1 pKa = 7.81LYY3 pKa = 10.93ADD5 pKa = 4.57IYY7 pKa = 10.9RR8 pKa = 11.84DD9 pKa = 3.42FGTVSFHH16 pKa = 6.72AGVGRR21 pKa = 11.84SVIDD25 pKa = 4.42EE26 pKa = 4.01NVEE29 pKa = 3.79WSFLLSLLLDD39 pKa = 3.51MCFDD43 pKa = 5.56PDD45 pKa = 4.02PSLPPHH51 pKa = 7.3LDD53 pKa = 3.31PGTTDD58 pKa = 3.59LFFGIASFQCTNEE71 pKa = 3.75LSTLIAA77 pKa = 4.57

Molecular weight: 8.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V4L3H1|V4L3H1_9DELT Uncharacterized protein OS=Desulfofustis sp. PB-SRB1 OX=1385624 GN=N839_09680 PE=4 SV=1
MM1 pKa = 7.48GGGVNSRR8 pKa = 11.84SISVLLNALGRR19 pKa = 11.84VRR21 pKa = 11.84PLFGKK26 pKa = 8.52STVANGLVVMWRR38 pKa = 11.84LLVRR42 pKa = 4.26

Molecular weight: 4.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3578

0

3578

1046809

29

2855

292.6

32.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.394 ± 0.043

1.266 ± 0.017

5.464 ± 0.035

6.159 ± 0.041

4.249 ± 0.032

7.758 ± 0.036

2.363 ± 0.021

6.635 ± 0.037

4.023 ± 0.036

10.35 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.599 ± 0.021

3.112 ± 0.021

4.537 ± 0.027

3.466 ± 0.026

6.163 ± 0.033

5.76 ± 0.028

5.466 ± 0.024

7.121 ± 0.035

1.179 ± 0.017

2.935 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski