Arthrobacter crystallopoietes

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Micrococcaceae; Arthrobacter

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4622 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H1EZC7|A0A1H1EZC7_9MICC 3-oxoadipate enol-lactonase OS=Arthrobacter crystallopoietes OX=37928 GN=SAMN04489742_3168 PE=4 SV=1
MM1 pKa = 7.35RR2 pKa = 11.84TNASTVVKK10 pKa = 9.89VFSVLATGTLALTACGGGSSTDD32 pKa = 3.48GGSPAADD39 pKa = 3.41GNEE42 pKa = 4.19LGLVTPGTLTVCSDD56 pKa = 2.76IPYY59 pKa = 9.82PPFEE63 pKa = 4.11FEE65 pKa = 4.57EE66 pKa = 4.34NGEE69 pKa = 4.1YY70 pKa = 9.8TGYY73 pKa = 11.36DD74 pKa = 2.87MDD76 pKa = 5.96LIRR79 pKa = 11.84EE80 pKa = 4.19IATGMGLEE88 pKa = 4.18TQIQDD93 pKa = 3.45VGFDD97 pKa = 3.59ALQSGVVLASGQCDD111 pKa = 2.83IGASAMTITEE121 pKa = 4.03EE122 pKa = 4.73RR123 pKa = 11.84EE124 pKa = 3.8EE125 pKa = 4.04NLDD128 pKa = 3.54FSEE131 pKa = 5.13PYY133 pKa = 10.14YY134 pKa = 11.08DD135 pKa = 4.06SLQSLLVPADD145 pKa = 3.75SDD147 pKa = 3.38IKK149 pKa = 11.16AIGDD153 pKa = 3.74LAGKK157 pKa = 9.84SVGVQQGTTGEE168 pKa = 4.17AYY170 pKa = 9.32TRR172 pKa = 11.84EE173 pKa = 4.12NVPADD178 pKa = 3.84TEE180 pKa = 4.23VRR182 pKa = 11.84AYY184 pKa = 9.91PSDD187 pKa = 4.28AEE189 pKa = 4.7LFPALQSGGVDD200 pKa = 3.88AVLQDD205 pKa = 3.72LPVNLGHH212 pKa = 7.63LEE214 pKa = 4.03GGKK217 pKa = 9.79YY218 pKa = 9.97EE219 pKa = 4.17IVEE222 pKa = 4.43EE223 pKa = 4.45YY224 pKa = 8.39PTDD227 pKa = 3.48EE228 pKa = 4.48SYY230 pKa = 11.73GFAVKK235 pKa = 10.65EE236 pKa = 4.13EE237 pKa = 4.28GSEE240 pKa = 4.08ALLEE244 pKa = 4.23AVNAEE249 pKa = 4.09LAEE252 pKa = 4.23LRR254 pKa = 11.84DD255 pKa = 3.5SGKK258 pKa = 10.07YY259 pKa = 7.88QEE261 pKa = 5.62IYY263 pKa = 10.83DD264 pKa = 4.37KK265 pKa = 11.54YY266 pKa = 9.33FTQQ269 pKa = 4.4

Molecular weight: 28.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H1BMJ5|A0A1H1BMJ5_9MICC DNA-binding transcriptional regulator IclR family OS=Arthrobacter crystallopoietes OX=37928 GN=SAMN04489742_1483 PE=4 SV=1
MM1 pKa = 7.41HH2 pKa = 7.02SAATSRR8 pKa = 11.84LAPVRR13 pKa = 11.84LLRR16 pKa = 11.84AAAVSATVLSLAAAAHH32 pKa = 5.24VAGGGTMPAAGVVIGLSVLTLLPVTLLAGRR62 pKa = 11.84KK63 pKa = 9.06LGPTTMAAILAVSQLALHH81 pKa = 5.8QAFTVLSTTAVCTEE95 pKa = 4.5AGHH98 pKa = 6.2HH99 pKa = 6.59AYY101 pKa = 10.22AGAGSLEE108 pKa = 4.4CVATAAGAAGAAVHH122 pKa = 6.7GPAEE126 pKa = 4.38HH127 pKa = 6.33GAAMVVAHH135 pKa = 6.24VLAAALTGLLLVRR148 pKa = 11.84GEE150 pKa = 4.01EE151 pKa = 4.33AVWALAAWLRR161 pKa = 11.84PLAKK165 pKa = 10.26LPSAVVLPILPRR177 pKa = 11.84PAMPPGGALLRR188 pKa = 11.84PRR190 pKa = 11.84TLCQLKK196 pKa = 10.5LEE198 pKa = 4.61PLRR201 pKa = 11.84GPPALSFHH209 pKa = 6.83

Molecular weight: 20.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4622

0

4622

1477220

30

2027

319.6

34.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.667 ± 0.055

0.672 ± 0.01

5.625 ± 0.025

6.115 ± 0.039

3.187 ± 0.023

8.966 ± 0.032

2.076 ± 0.016

4.537 ± 0.026

2.785 ± 0.024

10.252 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.108 ± 0.015

2.532 ± 0.019

5.425 ± 0.024

3.296 ± 0.021

6.492 ± 0.032

5.788 ± 0.022

5.783 ± 0.025

8.13 ± 0.03

1.424 ± 0.013

2.14 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski