Lysobacter sp. WF-2
Average proteome isoelectric point is 7.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2414 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A372DR64|A0A372DR64_9GAMM Flagellar basal-body rod protein FlgC OS=Lysobacter sp. WF-2 OX=2303539 GN=flgC PE=3 SV=1
MM1 pKa = 7.06 NAAVSTAPAPAAYY14 pKa = 7.36 RR15 pKa = 11.84 TWMCVVCGFVYY26 pKa = 10.69 DD27 pKa = 4.34 EE28 pKa = 4.44 AAGLPEE34 pKa = 4.8 EE35 pKa = 4.73 GLAPGTRR42 pKa = 11.84 WEE44 pKa = 4.77 DD45 pKa = 3.78 VPDD48 pKa = 3.14 TWTCPDD54 pKa = 3.91 CGVTKK59 pKa = 10.79 DD60 pKa = 4.24 DD61 pKa = 4.4 FEE63 pKa = 4.51 MMALL67 pKa = 3.73
Molecular weight: 7.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.828
IPC2_protein 3.808
IPC_protein 3.681
Toseland 3.49
ProMoST 3.872
Dawson 3.681
Bjellqvist 3.846
Wikipedia 3.643
Rodwell 3.528
Grimsley 3.414
Solomon 3.643
Lehninger 3.605
Nozaki 3.834
DTASelect 3.999
Thurlkill 3.579
EMBOSS 3.656
Sillero 3.808
Patrickios 0.299
IPC_peptide 3.643
IPC2_peptide 3.783
IPC2.peptide.svr19 3.747
Protein with the highest isoelectric point:
>tr|A0A372DSJ9|A0A372DSJ9_9GAMM Elongation factor G OS=Lysobacter sp. WF-2 OX=2303539 GN=D0Y53_00830 PE=3 SV=1
MM1 pKa = 7.5 RR2 pKa = 11.84 RR3 pKa = 11.84 ASPRR7 pKa = 11.84 PRR9 pKa = 11.84 RR10 pKa = 11.84 RR11 pKa = 11.84 QARR14 pKa = 11.84 RR15 pKa = 11.84 RR16 pKa = 11.84 QARR19 pKa = 11.84 GRR21 pKa = 11.84 RR22 pKa = 11.84 ARR24 pKa = 11.84 SCRR27 pKa = 11.84 ARR29 pKa = 11.84 SRR31 pKa = 11.84 RR32 pKa = 11.84 APRR35 pKa = 11.84 RR36 pKa = 11.84 GASRR40 pKa = 11.84 HH41 pKa = 4.28 GRR43 pKa = 11.84 WRR45 pKa = 11.84 RR46 pKa = 11.84 RR47 pKa = 11.84 CYY49 pKa = 9.66 WPSAWPGNCSRR60 pKa = 11.84 HH61 pKa = 4.44 PHH63 pKa = 5.85 RR64 pKa = 11.84 RR65 pKa = 11.84 PGRR68 pKa = 11.84 RR69 pKa = 11.84 WRR71 pKa = 11.84 RR72 pKa = 11.84 RR73 pKa = 11.84 RR74 pKa = 11.84 RR75 pKa = 11.84 STARR79 pKa = 11.84 PRR81 pKa = 11.84 RR82 pKa = 3.7
Molecular weight: 10.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.473
IPC2_protein 11.111
IPC_protein 12.647
Toseland 12.808
ProMoST 13.305
Dawson 12.808
Bjellqvist 12.808
Wikipedia 13.29
Rodwell 12.31
Grimsley 12.852
Solomon 13.305
Lehninger 13.203
Nozaki 12.808
DTASelect 12.808
Thurlkill 12.808
EMBOSS 13.305
Sillero 12.808
Patrickios 12.018
IPC_peptide 13.305
IPC2_peptide 12.296
IPC2.peptide.svr19 9.165
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2414
0
2414
790810
33
2361
327.6
35.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.974 ± 0.086
0.852 ± 0.017
5.852 ± 0.038
4.989 ± 0.044
3.212 ± 0.029
8.729 ± 0.046
2.246 ± 0.021
3.762 ± 0.038
2.293 ± 0.042
11.359 ± 0.079
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.943 ± 0.022
2.022 ± 0.032
5.693 ± 0.041
3.704 ± 0.028
8.428 ± 0.063
4.44 ± 0.034
4.465 ± 0.037
7.273 ± 0.044
1.522 ± 0.026
2.243 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here