Lysobacter sp. WF-2

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Xanthomonadaceae; Lysobacter; unclassified Lysobacter

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2414 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A372DR64|A0A372DR64_9GAMM Flagellar basal-body rod protein FlgC OS=Lysobacter sp. WF-2 OX=2303539 GN=flgC PE=3 SV=1
MM1 pKa = 7.06NAAVSTAPAPAAYY14 pKa = 7.36RR15 pKa = 11.84TWMCVVCGFVYY26 pKa = 10.69DD27 pKa = 4.34EE28 pKa = 4.44AAGLPEE34 pKa = 4.8EE35 pKa = 4.73GLAPGTRR42 pKa = 11.84WEE44 pKa = 4.77DD45 pKa = 3.78VPDD48 pKa = 3.14TWTCPDD54 pKa = 3.91CGVTKK59 pKa = 10.79DD60 pKa = 4.24DD61 pKa = 4.4FEE63 pKa = 4.51MMALL67 pKa = 3.73

Molecular weight:
7.23 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A372DSJ9|A0A372DSJ9_9GAMM Elongation factor G OS=Lysobacter sp. WF-2 OX=2303539 GN=D0Y53_00830 PE=3 SV=1
MM1 pKa = 7.5RR2 pKa = 11.84RR3 pKa = 11.84ASPRR7 pKa = 11.84PRR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84QARR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84QARR19 pKa = 11.84GRR21 pKa = 11.84RR22 pKa = 11.84ARR24 pKa = 11.84SCRR27 pKa = 11.84ARR29 pKa = 11.84SRR31 pKa = 11.84RR32 pKa = 11.84APRR35 pKa = 11.84RR36 pKa = 11.84GASRR40 pKa = 11.84HH41 pKa = 4.28GRR43 pKa = 11.84WRR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84CYY49 pKa = 9.66WPSAWPGNCSRR60 pKa = 11.84HH61 pKa = 4.44PHH63 pKa = 5.85RR64 pKa = 11.84RR65 pKa = 11.84PGRR68 pKa = 11.84RR69 pKa = 11.84WRR71 pKa = 11.84RR72 pKa = 11.84RR73 pKa = 11.84RR74 pKa = 11.84RR75 pKa = 11.84STARR79 pKa = 11.84PRR81 pKa = 11.84RR82 pKa = 3.7

Molecular weight:
10.34 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2414

0

2414

790810

33

2361

327.6

35.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.974 ± 0.086

0.852 ± 0.017

5.852 ± 0.038

4.989 ± 0.044

3.212 ± 0.029

8.729 ± 0.046

2.246 ± 0.021

3.762 ± 0.038

2.293 ± 0.042

11.359 ± 0.079

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.943 ± 0.022

2.022 ± 0.032

5.693 ± 0.041

3.704 ± 0.028

8.428 ± 0.063

4.44 ± 0.034

4.465 ± 0.037

7.273 ± 0.044

1.522 ± 0.026

2.243 ± 0.032

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski