Amino acid dipepetide frequency for Lysobacter sp. WF-2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
24.914AlaAla: 24.914 ± 0.329
1.401AlaCys: 1.401 ± 0.051
8.279AlaAsp: 8.279 ± 0.114
7.136AlaGlu: 7.136 ± 0.15
4.399AlaPhe: 4.399 ± 0.083
13.173AlaGly: 13.173 ± 0.143
3.103AlaHis: 3.103 ± 0.061
5.572AlaIle: 5.572 ± 0.086
2.745AlaLys: 2.745 ± 0.089
18.809AlaLeu: 18.809 ± 0.256
3.275AlaMet: 3.275 ± 0.067
2.419AlaAsn: 2.419 ± 0.054
8.027AlaPro: 8.027 ± 0.144
6.4AlaGln: 6.4 ± 0.104
12.577AlaArg: 12.577 ± 0.179
6.137AlaSer: 6.137 ± 0.099
6.775AlaThr: 6.775 ± 0.107
9.599AlaVal: 9.599 ± 0.134
2.315AlaTrp: 2.315 ± 0.072
2.685AlaTyr: 2.685 ± 0.064
0.0AlaXaa: 0.0 ± 0.0
Cys
1.203CysAla: 1.203 ± 0.041
0.119CysCys: 0.119 ± 0.014
0.531CysAsp: 0.531 ± 0.03
0.427CysGlu: 0.427 ± 0.026
0.267CysPhe: 0.267 ± 0.021
0.953CysGly: 0.953 ± 0.041
0.231CysHis: 0.231 ± 0.019
0.31CysIle: 0.31 ± 0.02
0.169CysLys: 0.169 ± 0.015
0.75CysLeu: 0.75 ± 0.032
0.139CysMet: 0.139 ± 0.013
0.2CysAsn: 0.2 ± 0.017
0.441CysPro: 0.441 ± 0.023
0.205CysGln: 0.205 ± 0.017
0.711CysArg: 0.711 ± 0.028
0.445CysSer: 0.445 ± 0.023
0.411CysThr: 0.411 ± 0.026
0.688CysVal: 0.688 ± 0.031
0.115CysTrp: 0.115 ± 0.012
0.201CysTyr: 0.201 ± 0.017
0.0CysXaa: 0.0 ± 0.0
Asp
9.981AspAla: 9.981 ± 0.147
0.475AspCys: 0.475 ± 0.027
3.355AspAsp: 3.355 ± 0.078
2.882AspGlu: 2.882 ± 0.066
2.003AspPhe: 2.003 ± 0.056
6.103AspGly: 6.103 ± 0.109
1.038AspHis: 1.038 ± 0.038
2.183AspIle: 2.183 ± 0.055
1.487AspLys: 1.487 ± 0.051
5.587AspLeu: 5.587 ± 0.096
0.966AspMet: 0.966 ± 0.036
1.203AspAsn: 1.203 ± 0.055
3.987AspPro: 3.987 ± 0.067
1.371AspGln: 1.371 ± 0.05
4.181AspArg: 4.181 ± 0.08
2.351AspSer: 2.351 ± 0.052
2.719AspThr: 2.719 ± 0.063
3.842AspVal: 3.842 ± 0.077
1.1AspTrp: 1.1 ± 0.037
1.713AspTyr: 1.713 ± 0.049
0.0AspXaa: 0.0 ± 0.0
Glu
7.117GluAla: 7.117 ± 0.127
0.339GluCys: 0.339 ± 0.024
2.449GluAsp: 2.449 ± 0.065
2.146GluGlu: 2.146 ± 0.068
1.73GluPhe: 1.73 ± 0.047
3.44GluGly: 3.44 ± 0.076
1.375GluHis: 1.375 ± 0.046
2.37GluIle: 2.37 ± 0.068
1.171GluLys: 1.171 ± 0.045
6.017GluLeu: 6.017 ± 0.1
0.986GluMet: 0.986 ± 0.037
0.904GluAsn: 0.904 ± 0.035
2.47GluPro: 2.47 ± 0.061
2.298GluGln: 2.298 ± 0.071
5.358GluArg: 5.358 ± 0.102
2.108GluSer: 2.108 ± 0.059
2.285GluThr: 2.285 ± 0.056
3.697GluVal: 3.697 ± 0.075
0.546GluTrp: 0.546 ± 0.026
1.087GluTyr: 1.087 ± 0.034
0.0GluXaa: 0.0 ± 0.0
Phe
4.771PheAla: 4.771 ± 0.075
0.316PheCys: 0.316 ± 0.023
2.624PheAsp: 2.624 ± 0.062
1.76PheGlu: 1.76 ± 0.05
1.087PhePhe: 1.087 ± 0.043
3.299PheGly: 3.299 ± 0.078
0.754PheHis: 0.754 ± 0.03
0.966PheIle: 0.966 ± 0.038
0.711PheLys: 0.711 ± 0.037
3.031PheLeu: 3.031 ± 0.065
0.564PheMet: 0.564 ± 0.028
0.821PheAsn: 0.821 ± 0.033
1.399PhePro: 1.399 ± 0.048
0.85PheGln: 0.85 ± 0.035
2.295PheArg: 2.295 ± 0.065
1.559PheSer: 1.559 ± 0.049
1.462PheThr: 1.462 ± 0.048
2.625PheVal: 2.625 ± 0.058
0.499PheTrp: 0.499 ± 0.027
0.723PheTyr: 0.723 ± 0.03
0.0PheXaa: 0.0 ± 0.0
Gly
10.814GlyAla: 10.814 ± 0.135
0.889GlyCys: 0.889 ± 0.038
5.202GlyAsp: 5.202 ± 0.098
4.81GlyGlu: 4.81 ± 0.089
3.313GlyPhe: 3.313 ± 0.065
7.529GlyGly: 7.529 ± 0.112
2.165GlyHis: 2.165 ± 0.055
3.897GlyIle: 3.897 ± 0.09
2.811GlyLys: 2.811 ± 0.078
9.05GlyLeu: 9.05 ± 0.105
2.088GlyMet: 2.088 ± 0.05
2.229GlyAsn: 2.229 ± 0.069
3.327GlyPro: 3.327 ± 0.063
2.982GlyGln: 2.982 ± 0.062
6.954GlyArg: 6.954 ± 0.117
4.597GlySer: 4.597 ± 0.093
4.093GlyThr: 4.093 ± 0.084
6.447GlyVal: 6.447 ± 0.117
1.684GlyTrp: 1.684 ± 0.046
2.411GlyTyr: 2.411 ± 0.056
0.0GlyXaa: 0.0 ± 0.0
His
3.582HisAla: 3.582 ± 0.077
0.309HisCys: 0.309 ± 0.018
1.383HisAsp: 1.383 ± 0.046
1.053HisGlu: 1.053 ± 0.038
0.732HisPhe: 0.732 ± 0.032
2.552HisGly: 2.552 ± 0.071
0.525HisHis: 0.525 ± 0.028
0.701HisIle: 0.701 ± 0.028
0.499HisLys: 0.499 ± 0.021
2.079HisLeu: 2.079 ± 0.058
0.367HisMet: 0.367 ± 0.02
0.438HisAsn: 0.438 ± 0.023
1.574HisPro: 1.574 ± 0.048
0.529HisGln: 0.529 ± 0.027
1.705HisArg: 1.705 ± 0.043
0.837HisSer: 0.837 ± 0.031
0.913HisThr: 0.913 ± 0.031
1.6HisVal: 1.6 ± 0.048
0.465HisTrp: 0.465 ± 0.025
0.615HisTyr: 0.615 ± 0.033
0.0HisXaa: 0.0 ± 0.0
Ile
6.688IleAla: 6.688 ± 0.099
0.295IleCys: 0.295 ± 0.021
3.085IleAsp: 3.085 ± 0.067
2.638IleGlu: 2.638 ± 0.073
0.972IlePhe: 0.972 ± 0.038
3.978IleGly: 3.978 ± 0.088
0.727IleHis: 0.727 ± 0.031
0.932IleIle: 0.932 ± 0.043
0.857IleLys: 0.857 ± 0.038
2.877IleLeu: 2.877 ± 0.075
0.488IleMet: 0.488 ± 0.029
0.928IleAsn: 0.928 ± 0.04
1.957IlePro: 1.957 ± 0.05
0.998IleGln: 0.998 ± 0.036
2.638IleArg: 2.638 ± 0.059
1.544IleSer: 1.544 ± 0.054
1.643IleThr: 1.643 ± 0.059
3.307IleVal: 3.307 ± 0.075
0.367IleTrp: 0.367 ± 0.021
0.702IleTyr: 0.702 ± 0.032
0.0IleXaa: 0.0 ± 0.0
Lys
2.929LysAla: 2.929 ± 0.088
0.125LysCys: 0.125 ± 0.014
1.203LysAsp: 1.203 ± 0.042
0.864LysGlu: 0.864 ± 0.041
0.637LysPhe: 0.637 ± 0.031
1.672LysGly: 1.672 ± 0.055
0.501LysHis: 0.501 ± 0.025
0.948LysIle: 0.948 ± 0.044
0.757LysLys: 0.757 ± 0.056
2.76LysLeu: 2.76 ± 0.073
0.517LysMet: 0.517 ± 0.028
0.463LysAsn: 0.463 ± 0.03
1.692LysPro: 1.692 ± 0.048
0.97LysGln: 0.97 ± 0.041
1.883LysArg: 1.883 ± 0.053
1.141LysSer: 1.141 ± 0.042
1.283LysThr: 1.283 ± 0.051
1.782LysVal: 1.782 ± 0.062
0.247LysTrp: 0.247 ± 0.017
0.555LysTyr: 0.555 ± 0.026
0.0LysXaa: 0.0 ± 0.0
Leu
18.386LeuAla: 18.386 ± 0.226
0.981LeuCys: 0.981 ± 0.033
7.005LeuAsp: 7.005 ± 0.115
5.367LeuGlu: 5.367 ± 0.096
3.336LeuPhe: 3.336 ± 0.074
9.263LeuGly: 9.263 ± 0.134
2.691LeuHis: 2.691 ± 0.064
3.245LeuIle: 3.245 ± 0.069
2.578LeuLys: 2.578 ± 0.062
13.709LeuLeu: 13.709 ± 0.219
1.787LeuMet: 1.787 ± 0.047
1.893LeuAsn: 1.893 ± 0.046
7.201LeuPro: 7.201 ± 0.11
4.657LeuGln: 4.657 ± 0.085
10.246LeuArg: 10.246 ± 0.147
5.043LeuSer: 5.043 ± 0.075
4.556LeuThr: 4.556 ± 0.08
7.951LeuVal: 7.951 ± 0.109
1.512LeuTrp: 1.512 ± 0.053
2.179LeuTyr: 2.179 ± 0.051
0.0LeuXaa: 0.0 ± 0.0
Met
2.534MetAla: 2.534 ± 0.059
0.129MetCys: 0.129 ± 0.012
0.995MetAsp: 0.995 ± 0.041
0.795MetGlu: 0.795 ± 0.033
0.546MetPhe: 0.546 ± 0.029
1.353MetGly: 1.353 ± 0.043
0.425MetHis: 0.425 ± 0.024
0.733MetIle: 0.733 ± 0.033
0.603MetLys: 0.603 ± 0.028
2.233MetLeu: 2.233 ± 0.055
0.345MetMet: 0.345 ± 0.022
0.591MetAsn: 0.591 ± 0.03
1.406MetPro: 1.406 ± 0.048
0.86MetGln: 0.86 ± 0.032
1.744MetArg: 1.744 ± 0.046
1.227MetSer: 1.227 ± 0.044
1.133MetThr: 1.133 ± 0.037
1.277MetVal: 1.277 ± 0.043
0.152MetTrp: 0.152 ± 0.014
0.344MetTyr: 0.344 ± 0.02
0.0MetXaa: 0.0 ± 0.0
Asn
2.987AsnAla: 2.987 ± 0.068
0.175AsnCys: 0.175 ± 0.014
1.108AsnAsp: 1.108 ± 0.042
0.873AsnGlu: 0.873 ± 0.041
0.732AsnPhe: 0.732 ± 0.03
1.779AsnGly: 1.779 ± 0.058
0.421AsnHis: 0.421 ± 0.021
0.869AsnIle: 0.869 ± 0.036
0.499AsnLys: 0.499 ± 0.027
2.21AsnLeu: 2.21 ± 0.052
0.314AsnMet: 0.314 ± 0.021
0.454AsnAsn: 0.454 ± 0.027
1.569AsnPro: 1.569 ± 0.043
0.545AsnGln: 0.545 ± 0.03
1.574AsnArg: 1.574 ± 0.046
0.785AsnSer: 0.785 ± 0.056
0.929AsnThr: 0.929 ± 0.036
1.59AsnVal: 1.59 ± 0.051
0.297AsnTrp: 0.297 ± 0.017
0.51AsnTyr: 0.51 ± 0.026
0.001AsnXaa: 0.001 ± 0.001
Pro
9.414ProAla: 9.414 ± 0.147
0.34ProCys: 0.34 ± 0.024
3.594ProAsp: 3.594 ± 0.072
3.235ProGlu: 3.235 ± 0.065
1.545ProPhe: 1.545 ± 0.048
5.398ProGly: 5.398 ± 0.105
1.155ProHis: 1.155 ± 0.036
1.73ProIle: 1.73 ± 0.052
1.071ProLys: 1.071 ± 0.044
6.008ProLeu: 6.008 ± 0.104
1.295ProMet: 1.295 ± 0.039
0.917ProAsn: 0.917 ± 0.031
3.598ProPro: 3.598 ± 0.088
2.585ProGln: 2.585 ± 0.057
4.336ProArg: 4.336 ± 0.103
2.272ProSer: 2.272 ± 0.054
2.243ProThr: 2.243 ± 0.054
4.114ProVal: 4.114 ± 0.08
0.908ProTrp: 0.908 ± 0.033
1.176ProTyr: 1.176 ± 0.04
0.0ProXaa: 0.0 ± 0.0
Gln
6.175GlnAla: 6.175 ± 0.1
0.23GlnCys: 0.23 ± 0.018
1.497GlnAsp: 1.497 ± 0.044
1.287GlnGlu: 1.287 ± 0.046
1.028GlnPhe: 1.028 ± 0.041
2.556GlnGly: 2.556 ± 0.061
0.818GlnHis: 0.818 ± 0.031
1.291GlnIle: 1.291 ± 0.043
0.761GlnLys: 0.761 ± 0.03
4.459GlnLeu: 4.459 ± 0.079
0.659GlnMet: 0.659 ± 0.032
0.522GlnAsn: 0.522 ± 0.028
2.457GlnPro: 2.457 ± 0.062
1.802GlnGln: 1.802 ± 0.05
4.162GlnArg: 4.162 ± 0.095
1.498GlnSer: 1.498 ± 0.051
1.444GlnThr: 1.444 ± 0.051
3.027GlnVal: 3.027 ± 0.058
0.611GlnTrp: 0.611 ± 0.032
0.752GlnTyr: 0.752 ± 0.034
0.0GlnXaa: 0.0 ± 0.0
Arg
11.038ArgAla: 11.038 ± 0.168
0.655ArgCys: 0.655 ± 0.033
4.941ArgAsp: 4.941 ± 0.081
5.119ArgGlu: 5.119 ± 0.096
3.068ArgPhe: 3.068 ± 0.069
6.428ArgGly: 6.428 ± 0.098
2.183ArgHis: 2.183 ± 0.056
4.28ArgIle: 4.28 ± 0.071
1.973ArgLys: 1.973 ± 0.053
9.575ArgLeu: 9.575 ± 0.143
1.949ArgMet: 1.949 ± 0.045
1.902ArgAsn: 1.902 ± 0.049
4.098ArgPro: 4.098 ± 0.082
3.259ArgGln: 3.259 ± 0.072
7.227ArgArg: 7.227 ± 0.12
3.596ArgSer: 3.596 ± 0.068
3.427ArgThr: 3.427 ± 0.06
5.652ArgVal: 5.652 ± 0.088
1.598ArgTrp: 1.598 ± 0.053
2.317ArgTyr: 2.317 ± 0.061
0.0ArgXaa: 0.0 ± 0.0
Ser
5.943SerAla: 5.943 ± 0.101
0.381SerCys: 0.381 ± 0.024
2.439SerAsp: 2.439 ± 0.054
1.971SerGlu: 1.971 ± 0.05
1.547SerPhe: 1.547 ± 0.046
4.933SerGly: 4.933 ± 0.096
0.976SerHis: 0.976 ± 0.038
1.756SerIle: 1.756 ± 0.059
1.012SerLys: 1.012 ± 0.036
4.78SerLeu: 4.78 ± 0.077
0.84SerMet: 0.84 ± 0.034
1.027SerAsn: 1.027 ± 0.037
2.434SerPro: 2.434 ± 0.064
1.486SerGln: 1.486 ± 0.042
3.489SerArg: 3.489 ± 0.061
2.188SerSer: 2.188 ± 0.055
2.202SerThr: 2.202 ± 0.061
3.24SerVal: 3.24 ± 0.07
0.613SerTrp: 0.613 ± 0.037
1.141SerTyr: 1.141 ± 0.042
0.0SerXaa: 0.0 ± 0.0
Thr
5.953ThrAla: 5.953 ± 0.088
0.378ThrCys: 0.378 ± 0.024
2.261ThrAsp: 2.261 ± 0.061
1.788ThrGlu: 1.788 ± 0.043
1.283ThrPhe: 1.283 ± 0.041
4.269ThrGly: 4.269 ± 0.091
0.974ThrHis: 0.974 ± 0.035
1.801ThrIle: 1.801 ± 0.055
0.744ThrLys: 0.744 ± 0.039
6.031ThrLeu: 6.031 ± 0.086
0.807ThrMet: 0.807 ± 0.034
0.77ThrAsn: 0.77 ± 0.034
3.244ThrPro: 3.244 ± 0.062
1.459ThrGln: 1.459 ± 0.046
3.651ThrArg: 3.651 ± 0.07
1.821ThrSer: 1.821 ± 0.057
2.176ThrThr: 2.176 ± 0.063
3.763ThrVal: 3.763 ± 0.068
0.61ThrTrp: 0.61 ± 0.024
0.871ThrTyr: 0.871 ± 0.042
0.0ThrXaa: 0.0 ± 0.0
Val
10.788ValAla: 10.788 ± 0.144
0.637ValCys: 0.637 ± 0.032
4.551ValAsp: 4.551 ± 0.08
4.248ValGlu: 4.248 ± 0.073
2.475ValPhe: 2.475 ± 0.066
5.516ValGly: 5.516 ± 0.097
1.563ValHis: 1.563 ± 0.045
2.72ValIle: 2.72 ± 0.07
1.625ValLys: 1.625 ± 0.056
8.656ValLeu: 8.656 ± 0.108
1.273ValMet: 1.273 ± 0.042
1.663ValAsn: 1.663 ± 0.057
3.988ValPro: 3.988 ± 0.076
2.262ValGln: 2.262 ± 0.05
5.727ValArg: 5.727 ± 0.091
3.39ValSer: 3.39 ± 0.077
3.231ValThr: 3.231 ± 0.073
6.058ValVal: 6.058 ± 0.106
0.831ValTrp: 0.831 ± 0.04
1.524ValTyr: 1.524 ± 0.044
0.0ValXaa: 0.0 ± 0.0
Trp
1.332TrpAla: 1.332 ± 0.046
0.149TrpCys: 0.149 ± 0.014
0.68TrpAsp: 0.68 ± 0.029
0.501TrpGlu: 0.501 ± 0.025
0.582TrpPhe: 0.582 ± 0.027
0.913TrpGly: 0.913 ± 0.038
0.411TrpHis: 0.411 ± 0.023
0.675TrpIle: 0.675 ± 0.034
0.414TrpLys: 0.414 ± 0.028
2.466TrpLeu: 2.466 ± 0.072
0.383TrpMet: 0.383 ± 0.022
0.395TrpAsn: 0.395 ± 0.023
0.885TrpPro: 0.885 ± 0.036
0.774TrpGln: 0.774 ± 0.03
1.64TrpArg: 1.64 ± 0.053
0.809TrpSer: 0.809 ± 0.038
0.695TrpThr: 0.695 ± 0.032
0.814TrpVal: 0.814 ± 0.033
0.363TrpTrp: 0.363 ± 0.022
0.34TrpTyr: 0.34 ± 0.026
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.19TyrAla: 3.19 ± 0.076
0.193TyrCys: 0.193 ± 0.017
1.342TyrAsp: 1.342 ± 0.05
0.993TyrGlu: 0.993 ± 0.04
0.833TyrPhe: 0.833 ± 0.032
2.188TyrGly: 2.188 ± 0.07
0.424TyrHis: 0.424 ± 0.021
0.642TyrIle: 0.642 ± 0.028
0.44TyrLys: 0.44 ± 0.028
2.503TyrLeu: 2.503 ± 0.058
0.383TyrMet: 0.383 ± 0.023
0.484TyrAsn: 0.484 ± 0.025
1.177TyrPro: 1.177 ± 0.043
0.747TyrGln: 0.747 ± 0.033
2.188TyrArg: 2.188 ± 0.051
1.048TyrSer: 1.048 ± 0.051
1.032TyrThr: 1.032 ± 0.039
1.653TyrVal: 1.653 ± 0.05
0.386TyrTrp: 0.386 ± 0.021
0.579TyrTyr: 0.579 ± 0.03
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.001XaaTrp: 0.001 ± 0.001
0.0XaaTyr: 0.0 ± 0.0
0.003XaaXaa: 0.003 ± 0.002
Statistics based on 2414 proteins (790810 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski