Lampropedia puyangensis
Average proteome isoelectric point is 6.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3366 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4S8FAV4|A0A4S8FAV4_9BURK DUF3567 domain-containing protein OS=Lampropedia puyangensis OX=1330072 GN=E9531_06455 PE=4 SV=1
MM1 pKa = 7.19 NAVAEE6 pKa = 4.28 QAQTTDD12 pKa = 3.75 LPTPIIFTDD21 pKa = 3.46 SAAAKK26 pKa = 10.02 VADD29 pKa = 5.45 LIAEE33 pKa = 4.31 EE34 pKa = 4.82 GNPDD38 pKa = 2.99 LKK40 pKa = 11.13 LRR42 pKa = 11.84 VFVQGGGCSGFQYY55 pKa = 10.94 GFTFDD60 pKa = 4.65 EE61 pKa = 4.88 IANEE65 pKa = 4.77 DD66 pKa = 4.07 DD67 pKa = 3.27 TTMTKK72 pKa = 10.66 NGVSLLIDD80 pKa = 3.54 AMSYY84 pKa = 10.32 QYY86 pKa = 11.5 LVGAEE91 pKa = 3.79 IDD93 pKa = 3.91 YY94 pKa = 11.36 KK95 pKa = 11.19 EE96 pKa = 4.67 DD97 pKa = 3.23 LQGAQFVIKK106 pKa = 10.4 NPNATTTCGCGSSFSTT122 pKa = 4.55
Molecular weight: 12.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.748
IPC2_protein 3.872
IPC_protein 3.821
Toseland 3.605
ProMoST 3.986
Dawson 3.808
Bjellqvist 3.973
Wikipedia 3.757
Rodwell 3.643
Grimsley 3.528
Solomon 3.795
Lehninger 3.745
Nozaki 3.935
DTASelect 4.151
Thurlkill 3.681
EMBOSS 3.757
Sillero 3.935
Patrickios 1.888
IPC_peptide 3.783
IPC2_peptide 3.91
IPC2.peptide.svr19 3.844
Protein with the highest isoelectric point:
>tr|A0A4S8FBL8|A0A4S8FBL8_9BURK Bacterioferritin OS=Lampropedia puyangensis OX=1330072 GN=bfr PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.07 QPSKK9 pKa = 9.07 IRR11 pKa = 11.84 RR12 pKa = 11.84 ARR14 pKa = 11.84 THH16 pKa = 5.91 GFLVRR21 pKa = 11.84 MKK23 pKa = 9.7 TRR25 pKa = 11.84 GGRR28 pKa = 11.84 AVINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.67 GRR39 pKa = 11.84 KK40 pKa = 8.84 RR41 pKa = 11.84 LALSS45 pKa = 3.39
Molecular weight: 5.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3366
0
3366
1140859
15
2578
338.9
36.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.926 ± 0.055
0.911 ± 0.012
4.881 ± 0.027
5.092 ± 0.04
3.503 ± 0.023
7.619 ± 0.042
2.529 ± 0.024
4.902 ± 0.03
3.505 ± 0.038
10.63 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.495 ± 0.021
3.145 ± 0.028
4.927 ± 0.029
5.265 ± 0.046
5.769 ± 0.036
5.953 ± 0.035
5.549 ± 0.043
7.432 ± 0.04
1.575 ± 0.019
2.392 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here