Erwinia mallotivora
Average proteome isoelectric point is 6.64
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3959 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A014NB36|A0A014NB36_9GAMM DeoR faimly transcriptional regulator OS=Erwinia mallotivora OX=69222 GN=BG55_04100 PE=4 SV=1
MM1 pKa = 7.55 KK2 pKa = 10.38 YY3 pKa = 10.57 DD4 pKa = 3.39 VATLISGMLSDD15 pKa = 5.46 AGLSDD20 pKa = 4.44 IVDD23 pKa = 3.78 SDD25 pKa = 4.35 LSNHH29 pKa = 5.18 STISLNMKK37 pKa = 10.37 DD38 pKa = 5.48 DD39 pKa = 3.56 IPAINIKK46 pKa = 9.63 TDD48 pKa = 3.71 DD49 pKa = 4.09 DD50 pKa = 4.54 EE51 pKa = 4.81 VWVWTKK57 pKa = 11.03 LCDD60 pKa = 3.77 YY61 pKa = 9.76 SPSSMSYY68 pKa = 9.39 CSEE71 pKa = 4.19 NIFTVMFNYY80 pKa = 10.61 NEE82 pKa = 3.91 AFFYY86 pKa = 10.62 AGQPCLYY93 pKa = 9.48 PIDD96 pKa = 4.55 GNLEE100 pKa = 3.56 LRR102 pKa = 11.84 ALVKK106 pKa = 10.74 DD107 pKa = 4.07 KK108 pKa = 10.92 FLEE111 pKa = 4.43 SPSAFMEE118 pKa = 4.09 VLDD121 pKa = 4.25 NFLSIMQEE129 pKa = 3.77 YY130 pKa = 9.91 RR131 pKa = 11.84 AVLMM135 pKa = 5.02
Molecular weight: 15.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.749
IPC2_protein 3.948
IPC_protein 3.923
Toseland 3.694
ProMoST 4.088
Dawson 3.91
Bjellqvist 4.075
Wikipedia 3.859
Rodwell 3.745
Grimsley 3.617
Solomon 3.897
Lehninger 3.859
Nozaki 4.037
DTASelect 4.279
Thurlkill 3.757
EMBOSS 3.872
Sillero 4.037
Patrickios 0.998
IPC_peptide 3.897
IPC2_peptide 4.012
IPC2.peptide.svr19 3.928
Protein with the highest isoelectric point:
>tr|A0A014M5I1|A0A014M5I1_9GAMM ABC transporter substrate-binding protein OS=Erwinia mallotivora OX=69222 GN=BG55_01915 PE=4 SV=1
MM1 pKa = 7.59 EE2 pKa = 5.39 FYY4 pKa = 10.43 EE5 pKa = 4.33 NRR7 pKa = 11.84 SARR10 pKa = 11.84 PFKK13 pKa = 10.42 EE14 pKa = 4.0 SPFRR18 pKa = 11.84 LICQLPYY25 pKa = 11.02 RR26 pKa = 11.84 MWKK29 pKa = 8.6 SWRR32 pKa = 11.84 LRR34 pKa = 11.84 TLTRR38 pKa = 11.84 RR39 pKa = 11.84 ALSRR43 pKa = 11.84 LDD45 pKa = 3.23 KK46 pKa = 11.14 AQLRR50 pKa = 11.84 DD51 pKa = 3.27 IGLTCEE57 pKa = 3.83 DD58 pKa = 3.39 VNRR61 pKa = 11.84 FKK63 pKa = 11.28
Molecular weight: 7.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.353
IPC2_protein 9.619
IPC_protein 10.526
Toseland 10.716
ProMoST 10.57
Dawson 10.804
Bjellqvist 10.555
Wikipedia 11.038
Rodwell 10.935
Grimsley 10.847
Solomon 10.965
Lehninger 10.921
Nozaki 10.716
DTASelect 10.54
Thurlkill 10.716
EMBOSS 11.125
Sillero 10.745
Patrickios 10.716
IPC_peptide 10.965
IPC2_peptide 9.75
IPC2.peptide.svr19 8.623
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3959
0
3959
1244858
34
2587
314.4
34.77
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.731 ± 0.047
1.09 ± 0.013
5.152 ± 0.027
5.446 ± 0.043
3.834 ± 0.026
7.295 ± 0.043
2.308 ± 0.02
5.587 ± 0.033
4.09 ± 0.038
11.044 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.657 ± 0.02
3.77 ± 0.031
4.429 ± 0.025
4.829 ± 0.037
5.827 ± 0.034
6.438 ± 0.035
5.32 ± 0.028
6.91 ± 0.029
1.516 ± 0.018
2.726 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here