Amino acid dipepetide frequency for Achromatium sp. WMS3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.19AlaAla: 7.19 ± 0.12
0.884AlaCys: 0.884 ± 0.035
3.798AlaAsp: 3.798 ± 0.082
4.452AlaGlu: 4.452 ± 0.092
2.917AlaPhe: 2.917 ± 0.061
4.977AlaGly: 4.977 ± 0.108
1.67AlaHis: 1.67 ± 0.048
6.881AlaIle: 6.881 ± 0.102
5.0AlaLys: 5.0 ± 0.095
8.785AlaLeu: 8.785 ± 0.128
2.001AlaMet: 2.001 ± 0.058
3.912AlaAsn: 3.912 ± 0.071
2.855AlaPro: 2.855 ± 0.066
3.831AlaGln: 3.831 ± 0.1
3.609AlaArg: 3.609 ± 0.082
4.13AlaSer: 4.13 ± 0.07
5.349AlaThr: 5.349 ± 0.095
4.819AlaVal: 4.819 ± 0.077
1.096AlaTrp: 1.096 ± 0.044
2.457AlaTyr: 2.457 ± 0.057
0.0AlaXaa: 0.0 ± 0.0
Cys
0.73CysAla: 0.73 ± 0.03
0.17CysCys: 0.17 ± 0.016
0.529CysAsp: 0.529 ± 0.029
0.522CysGlu: 0.522 ± 0.026
0.432CysPhe: 0.432 ± 0.024
0.9CysGly: 0.9 ± 0.048
0.344CysHis: 0.344 ± 0.022
0.773CysIle: 0.773 ± 0.035
0.528CysLys: 0.528 ± 0.025
1.071CysLeu: 1.071 ± 0.036
0.264CysMet: 0.264 ± 0.016
0.566CysAsn: 0.566 ± 0.028
0.548CysPro: 0.548 ± 0.031
0.448CysGln: 0.448 ± 0.025
0.552CysArg: 0.552 ± 0.025
0.646CysSer: 0.646 ± 0.034
0.632CysThr: 0.632 ± 0.031
0.542CysVal: 0.542 ± 0.026
0.137CysTrp: 0.137 ± 0.014
0.414CysTyr: 0.414 ± 0.024
0.0CysXaa: 0.0 ± 0.0
Asp
4.146AspAla: 4.146 ± 0.089
0.511AspCys: 0.511 ± 0.03
2.559AspAsp: 2.559 ± 0.092
2.612AspGlu: 2.612 ± 0.073
2.296AspPhe: 2.296 ± 0.049
3.093AspGly: 3.093 ± 0.09
1.069AspHis: 1.069 ± 0.042
4.406AspIle: 4.406 ± 0.072
2.749AspLys: 2.749 ± 0.068
5.588AspLeu: 5.588 ± 0.089
1.059AspMet: 1.059 ± 0.037
2.42AspAsn: 2.42 ± 0.057
2.641AspPro: 2.641 ± 0.073
2.076AspGln: 2.076 ± 0.066
2.273AspArg: 2.273 ± 0.062
2.811AspSer: 2.811 ± 0.064
3.449AspThr: 3.449 ± 0.112
3.0AspVal: 3.0 ± 0.069
0.742AspTrp: 0.742 ± 0.03
1.891AspTyr: 1.891 ± 0.056
0.0AspXaa: 0.0 ± 0.0
Glu
4.462GluAla: 4.462 ± 0.087
0.51GluCys: 0.51 ± 0.025
2.455GluAsp: 2.455 ± 0.065
3.069GluGlu: 3.069 ± 0.075
2.074GluPhe: 2.074 ± 0.052
2.564GluGly: 2.564 ± 0.068
1.483GluHis: 1.483 ± 0.044
4.554GluIle: 4.554 ± 0.072
2.81GluLys: 2.81 ± 0.069
6.375GluLeu: 6.375 ± 0.105
1.21GluMet: 1.21 ± 0.041
2.469GluAsn: 2.469 ± 0.076
2.051GluPro: 2.051 ± 0.055
3.387GluGln: 3.387 ± 0.082
3.26GluArg: 3.26 ± 0.072
2.695GluSer: 2.695 ± 0.061
3.305GluThr: 3.305 ± 0.069
3.391GluVal: 3.391 ± 0.087
0.722GluTrp: 0.722 ± 0.028
1.865GluTyr: 1.865 ± 0.058
0.0GluXaa: 0.0 ± 0.0
Phe
3.182PheAla: 3.182 ± 0.075
0.493PheCys: 0.493 ± 0.023
2.145PheAsp: 2.145 ± 0.055
1.925PheGlu: 1.925 ± 0.059
1.695PhePhe: 1.695 ± 0.055
2.688PheGly: 2.688 ± 0.059
0.842PheHis: 0.842 ± 0.032
3.051PheIle: 3.051 ± 0.068
1.977PheLys: 1.977 ± 0.058
3.947PheLeu: 3.947 ± 0.091
0.933PheMet: 0.933 ± 0.039
1.886PheAsn: 1.886 ± 0.049
1.508PhePro: 1.508 ± 0.042
1.534PheGln: 1.534 ± 0.044
1.696PheArg: 1.696 ± 0.049
2.668PheSer: 2.668 ± 0.066
2.58PheThr: 2.58 ± 0.067
2.294PheVal: 2.294 ± 0.053
0.613PheTrp: 0.613 ± 0.028
1.38PheTyr: 1.38 ± 0.045
0.0PheXaa: 0.0 ± 0.0
Gly
4.466GlyAla: 4.466 ± 0.084
0.886GlyCys: 0.886 ± 0.04
2.937GlyAsp: 2.937 ± 0.077
2.914GlyGlu: 2.914 ± 0.067
2.664GlyPhe: 2.664 ± 0.07
4.173GlyGly: 4.173 ± 0.111
1.578GlyHis: 1.578 ± 0.052
5.437GlyIle: 5.437 ± 0.092
3.6GlyLys: 3.6 ± 0.065
6.625GlyLeu: 6.625 ± 0.111
1.567GlyMet: 1.567 ± 0.051
2.972GlyAsn: 2.972 ± 0.094
1.778GlyPro: 1.778 ± 0.053
2.772GlyGln: 2.772 ± 0.065
2.909GlyArg: 2.909 ± 0.07
3.571GlySer: 3.571 ± 0.094
3.982GlyThr: 3.982 ± 0.106
3.827GlyVal: 3.827 ± 0.083
0.93GlyTrp: 0.93 ± 0.037
2.357GlyTyr: 2.357 ± 0.058
0.0GlyXaa: 0.0 ± 0.0
His
1.575HisAla: 1.575 ± 0.044
0.353HisCys: 0.353 ± 0.022
1.014HisAsp: 1.014 ± 0.038
1.133HisGlu: 1.133 ± 0.043
0.921HisPhe: 0.921 ± 0.039
1.485HisGly: 1.485 ± 0.049
0.74HisHis: 0.74 ± 0.034
1.873HisIle: 1.873 ± 0.054
1.503HisLys: 1.503 ± 0.042
2.531HisLeu: 2.531 ± 0.057
0.361HisMet: 0.361 ± 0.021
1.339HisAsn: 1.339 ± 0.043
1.321HisPro: 1.321 ± 0.044
1.188HisGln: 1.188 ± 0.044
1.191HisArg: 1.191 ± 0.039
1.339HisSer: 1.339 ± 0.045
1.571HisThr: 1.571 ± 0.054
1.127HisVal: 1.127 ± 0.043
0.368HisTrp: 0.368 ± 0.021
1.018HisTyr: 1.018 ± 0.036
0.0HisXaa: 0.0 ± 0.0
Ile
7.26IleAla: 7.26 ± 0.106
0.823IleCys: 0.823 ± 0.033
4.596IleAsp: 4.596 ± 0.091
4.525IleGlu: 4.525 ± 0.081
3.066IlePhe: 3.066 ± 0.07
5.202IleGly: 5.202 ± 0.099
1.66IleHis: 1.66 ± 0.048
5.752IleIle: 5.752 ± 0.113
4.305IleLys: 4.305 ± 0.084
8.364IleLeu: 8.364 ± 0.139
1.508IleMet: 1.508 ± 0.047
3.87IleAsn: 3.87 ± 0.08
3.737IlePro: 3.737 ± 0.079
3.376IleGln: 3.376 ± 0.066
3.736IleArg: 3.736 ± 0.08
4.781IleSer: 4.781 ± 0.085
4.884IleThr: 4.884 ± 0.089
4.48IleVal: 4.48 ± 0.065
0.887IleTrp: 0.887 ± 0.03
2.282IleTyr: 2.282 ± 0.057
0.0IleXaa: 0.0 ± 0.0
Lys
4.428LysAla: 4.428 ± 0.089
0.437LysCys: 0.437 ± 0.027
2.995LysAsp: 2.995 ± 0.076
3.069LysGlu: 3.069 ± 0.071
2.007LysPhe: 2.007 ± 0.058
2.793LysGly: 2.793 ± 0.066
1.485LysHis: 1.485 ± 0.04
4.56LysIle: 4.56 ± 0.094
3.083LysLys: 3.083 ± 0.083
6.096LysLeu: 6.096 ± 0.106
1.084LysMet: 1.084 ± 0.04
2.775LysAsn: 2.775 ± 0.071
2.585LysPro: 2.585 ± 0.058
3.089LysGln: 3.089 ± 0.082
3.082LysArg: 3.082 ± 0.064
3.113LysSer: 3.113 ± 0.067
3.568LysThr: 3.568 ± 0.069
3.138LysVal: 3.138 ± 0.058
0.637LysTrp: 0.637 ± 0.028
1.82LysTyr: 1.82 ± 0.052
0.0LysXaa: 0.0 ± 0.0
Leu
9.6LeuAla: 9.6 ± 0.133
1.018LeuCys: 1.018 ± 0.037
6.007LeuAsp: 6.007 ± 0.109
6.54LeuGlu: 6.54 ± 0.133
4.008LeuPhe: 4.008 ± 0.095
6.752LeuGly: 6.752 ± 0.124
2.504LeuHis: 2.504 ± 0.056
7.489LeuIle: 7.489 ± 0.129
6.192LeuLys: 6.192 ± 0.1
11.096LeuLeu: 11.096 ± 0.183
2.15LeuMet: 2.15 ± 0.06
4.755LeuAsn: 4.755 ± 0.074
4.971LeuPro: 4.971 ± 0.087
5.388LeuGln: 5.388 ± 0.1
5.597LeuArg: 5.597 ± 0.103
6.15LeuSer: 6.15 ± 0.098
6.162LeuThr: 6.162 ± 0.088
6.002LeuVal: 6.002 ± 0.09
1.422LeuTrp: 1.422 ± 0.053
3.07LeuTyr: 3.07 ± 0.072
0.0LeuXaa: 0.0 ± 0.0
Met
1.828MetAla: 1.828 ± 0.05
0.143MetCys: 0.143 ± 0.015
1.18MetAsp: 1.18 ± 0.04
1.138MetGlu: 1.138 ± 0.041
0.743MetPhe: 0.743 ± 0.032
1.367MetGly: 1.367 ± 0.052
0.504MetHis: 0.504 ± 0.026
1.331MetIle: 1.331 ± 0.047
1.029MetLys: 1.029 ± 0.034
2.393MetLeu: 2.393 ± 0.06
0.485MetMet: 0.485 ± 0.028
1.059MetAsn: 1.059 ± 0.037
1.189MetPro: 1.189 ± 0.033
1.327MetGln: 1.327 ± 0.038
1.253MetArg: 1.253 ± 0.037
1.322MetSer: 1.322 ± 0.044
1.071MetThr: 1.071 ± 0.034
1.36MetVal: 1.36 ± 0.045
0.203MetTrp: 0.203 ± 0.016
0.52MetTyr: 0.52 ± 0.027
0.0MetXaa: 0.0 ± 0.0
Asn
3.604AsnAla: 3.604 ± 0.072
0.551AsnCys: 0.551 ± 0.031
2.156AsnAsp: 2.156 ± 0.075
2.179AsnGlu: 2.179 ± 0.062
1.923AsnPhe: 1.923 ± 0.057
2.908AsnGly: 2.908 ± 0.086
1.056AsnHis: 1.056 ± 0.038
4.364AsnIle: 4.364 ± 0.074
2.715AsnLys: 2.715 ± 0.068
5.248AsnLeu: 5.248 ± 0.094
1.032AsnMet: 1.032 ± 0.037
2.71AsnAsn: 2.71 ± 0.069
2.527AsnPro: 2.527 ± 0.059
2.326AsnGln: 2.326 ± 0.067
2.246AsnArg: 2.246 ± 0.059
2.9AsnSer: 2.9 ± 0.065
3.15AsnThr: 3.15 ± 0.081
2.562AsnVal: 2.562 ± 0.08
0.7AsnTrp: 0.7 ± 0.029
1.774AsnTyr: 1.774 ± 0.05
0.0AsnXaa: 0.0 ± 0.0
Pro
2.873ProAla: 2.873 ± 0.062
0.36ProCys: 0.36 ± 0.02
2.714ProAsp: 2.714 ± 0.068
3.128ProGlu: 3.128 ± 0.063
1.567ProPhe: 1.567 ± 0.039
2.567ProGly: 2.567 ± 0.068
0.987ProHis: 0.987 ± 0.033
3.545ProIle: 3.545 ± 0.073
2.86ProLys: 2.86 ± 0.066
4.052ProLeu: 4.052 ± 0.076
0.958ProMet: 0.958 ± 0.04
2.527ProAsn: 2.527 ± 0.059
2.015ProPro: 2.015 ± 0.068
2.226ProGln: 2.226 ± 0.057
1.594ProArg: 1.594 ± 0.044
2.281ProSer: 2.281 ± 0.059
2.742ProThr: 2.742 ± 0.07
2.571ProVal: 2.571 ± 0.065
0.631ProTrp: 0.631 ± 0.031
1.571ProTyr: 1.571 ± 0.047
0.0ProXaa: 0.0 ± 0.0
Gln
4.801GlnAla: 4.801 ± 0.104
0.459GlnCys: 0.459 ± 0.024
2.621GlnAsp: 2.621 ± 0.062
3.151GlnGlu: 3.151 ± 0.07
1.632GlnPhe: 1.632 ± 0.046
2.647GlnGly: 2.647 ± 0.071
1.486GlnHis: 1.486 ± 0.045
3.813GlnIle: 3.813 ± 0.069
2.415GlnLys: 2.415 ± 0.065
4.952GlnLeu: 4.952 ± 0.11
0.971GlnMet: 0.971 ± 0.039
2.165GlnAsn: 2.165 ± 0.056
2.067GlnPro: 2.067 ± 0.056
3.43GlnGln: 3.43 ± 0.105
2.918GlnArg: 2.918 ± 0.077
2.324GlnSer: 2.324 ± 0.062
2.842GlnThr: 2.842 ± 0.067
3.171GlnVal: 3.171 ± 0.063
0.61GlnTrp: 0.61 ± 0.027
1.463GlnTyr: 1.463 ± 0.051
0.0GlnXaa: 0.0 ± 0.0
Arg
3.017ArgAla: 3.017 ± 0.072
0.526ArgCys: 0.526 ± 0.026
2.37ArgAsp: 2.37 ± 0.058
2.629ArgGlu: 2.629 ± 0.065
2.34ArgPhe: 2.34 ± 0.054
2.563ArgGly: 2.563 ± 0.064
1.374ArgHis: 1.374 ± 0.045
3.981ArgIle: 3.981 ± 0.068
2.807ArgLys: 2.807 ± 0.07
5.858ArgLeu: 5.858 ± 0.106
1.237ArgMet: 1.237 ± 0.046
2.376ArgAsn: 2.376 ± 0.053
1.912ArgPro: 1.912 ± 0.054
2.384ArgGln: 2.384 ± 0.065
2.771ArgArg: 2.771 ± 0.072
2.673ArgSer: 2.673 ± 0.058
2.62ArgThr: 2.62 ± 0.056
2.721ArgVal: 2.721 ± 0.061
0.711ArgTrp: 0.711 ± 0.03
2.006ArgTyr: 2.006 ± 0.055
0.0ArgXaa: 0.0 ± 0.0
Ser
3.853SerAla: 3.853 ± 0.082
0.697SerCys: 0.697 ± 0.039
2.657SerAsp: 2.657 ± 0.063
2.908SerGlu: 2.908 ± 0.065
2.263SerPhe: 2.263 ± 0.051
4.226SerGly: 4.226 ± 0.092
1.344SerHis: 1.344 ± 0.045
4.499SerIle: 4.499 ± 0.088
3.427SerLys: 3.427 ± 0.077
5.887SerLeu: 5.887 ± 0.102
1.307SerMet: 1.307 ± 0.04
3.002SerAsn: 3.002 ± 0.076
2.377SerPro: 2.377 ± 0.061
2.736SerGln: 2.736 ± 0.061
2.562SerArg: 2.562 ± 0.064
3.67SerSer: 3.67 ± 0.085
3.258SerThr: 3.258 ± 0.08
3.136SerVal: 3.136 ± 0.066
0.822SerTrp: 0.822 ± 0.035
1.867SerTyr: 1.867 ± 0.05
0.0SerXaa: 0.0 ± 0.0
Thr
5.134ThrAla: 5.134 ± 0.107
0.556ThrCys: 0.556 ± 0.035
3.255ThrAsp: 3.255 ± 0.111
3.368ThrGlu: 3.368 ± 0.075
2.139ThrPhe: 2.139 ± 0.057
4.259ThrGly: 4.259 ± 0.109
1.344ThrHis: 1.344 ± 0.042
5.093ThrIle: 5.093 ± 0.096
3.598ThrLys: 3.598 ± 0.081
6.496ThrLeu: 6.496 ± 0.097
1.055ThrMet: 1.055 ± 0.036
3.15ThrAsn: 3.15 ± 0.071
3.053ThrPro: 3.053 ± 0.06
2.917ThrGln: 2.917 ± 0.062
2.477ThrArg: 2.477 ± 0.055
3.434ThrSer: 3.434 ± 0.072
3.941ThrThr: 3.941 ± 0.095
3.866ThrVal: 3.866 ± 0.132
0.823ThrTrp: 0.823 ± 0.034
1.85ThrTyr: 1.85 ± 0.052
0.0ThrXaa: 0.0 ± 0.0
Val
5.015ValAla: 5.015 ± 0.078
0.733ValCys: 0.733 ± 0.031
3.172ValAsp: 3.172 ± 0.101
3.149ValGlu: 3.149 ± 0.071
2.334ValPhe: 2.334 ± 0.064
3.803ValGly: 3.803 ± 0.081
1.233ValHis: 1.233 ± 0.041
4.418ValIle: 4.418 ± 0.086
2.861ValLys: 2.861 ± 0.065
6.103ValLeu: 6.103 ± 0.092
1.294ValMet: 1.294 ± 0.046
2.53ValAsn: 2.53 ± 0.089
2.557ValPro: 2.557 ± 0.051
2.527ValGln: 2.527 ± 0.064
2.674ValArg: 2.674 ± 0.062
3.283ValSer: 3.283 ± 0.075
4.187ValThr: 4.187 ± 0.112
3.583ValVal: 3.583 ± 0.086
0.728ValTrp: 0.728 ± 0.033
1.819ValTyr: 1.819 ± 0.046
0.0ValXaa: 0.0 ± 0.0
Trp
0.814TrpAla: 0.814 ± 0.032
0.195TrpCys: 0.195 ± 0.015
0.622TrpAsp: 0.622 ± 0.031
0.704TrpGlu: 0.704 ± 0.031
0.586TrpPhe: 0.586 ± 0.033
0.78TrpGly: 0.78 ± 0.034
0.457TrpHis: 0.457 ± 0.023
1.077TrpIle: 1.077 ± 0.043
0.614TrpLys: 0.614 ± 0.03
1.877TrpLeu: 1.877 ± 0.062
0.351TrpMet: 0.351 ± 0.019
0.584TrpAsn: 0.584 ± 0.024
0.548TrpPro: 0.548 ± 0.032
0.966TrpGln: 0.966 ± 0.033
0.672TrpArg: 0.672 ± 0.03
0.746TrpSer: 0.746 ± 0.03
0.569TrpThr: 0.569 ± 0.03
0.706TrpVal: 0.706 ± 0.03
0.24TrpTrp: 0.24 ± 0.019
0.479TrpTyr: 0.479 ± 0.029
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.634TyrAla: 2.634 ± 0.072
0.445TyrCys: 0.445 ± 0.027
1.61TyrAsp: 1.61 ± 0.042
1.597TyrGlu: 1.597 ± 0.049
1.431TyrPhe: 1.431 ± 0.046
2.195TyrGly: 2.195 ± 0.057
0.788TyrHis: 0.788 ± 0.033
2.197TyrIle: 2.197 ± 0.059
1.772TyrLys: 1.772 ± 0.056
3.531TyrLeu: 3.531 ± 0.068
0.618TyrMet: 0.618 ± 0.03
1.563TyrAsn: 1.563 ± 0.049
1.53TyrPro: 1.53 ± 0.047
2.01TyrGln: 2.01 ± 0.063
1.805TyrArg: 1.805 ± 0.048
1.962TyrSer: 1.962 ± 0.053
1.944TyrThr: 1.944 ± 0.053
1.705TyrVal: 1.705 ± 0.054
0.565TyrTrp: 0.565 ± 0.031
1.343TyrTyr: 1.343 ± 0.05
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3099 proteins (775224 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski