Vagococcus fluvialis bH819

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae; Vagococcus; Vagococcus fluvialis

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2822 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X6WLV3|A0A1X6WLV3_9ENTE Tyrosine-protein phosphatase OS=Vagococcus fluvialis bH819 OX=1255619 GN=FM121_04080 PE=3 SV=1
MM1 pKa = 7.36TDD3 pKa = 2.92KK4 pKa = 10.8HH5 pKa = 6.24VHH7 pKa = 6.94DD8 pKa = 5.17EE9 pKa = 4.41NCNHH13 pKa = 6.64DD14 pKa = 4.03HH15 pKa = 7.25DD16 pKa = 6.05HH17 pKa = 6.37IHH19 pKa = 6.99DD20 pKa = 4.5AEE22 pKa = 4.33DD23 pKa = 5.03LITLIDD29 pKa = 3.98DD30 pKa = 4.09EE31 pKa = 4.86GNEE34 pKa = 3.99VLFRR38 pKa = 11.84IHH40 pKa = 6.3LTIDD44 pKa = 3.2GQEE47 pKa = 4.29EE48 pKa = 4.31YY49 pKa = 11.2GKK51 pKa = 10.45DD52 pKa = 3.53YY53 pKa = 11.46VLLYY57 pKa = 10.64DD58 pKa = 4.64ASTPEE63 pKa = 3.92NEE65 pKa = 4.41EE66 pKa = 4.2VEE68 pKa = 4.44LLAYY72 pKa = 10.02AYY74 pKa = 7.14EE75 pKa = 4.27QKK77 pKa = 10.8DD78 pKa = 3.66GEE80 pKa = 4.64TEE82 pKa = 3.91GRR84 pKa = 11.84LTEE87 pKa = 4.26IEE89 pKa = 4.71TEE91 pKa = 4.49AEE93 pKa = 3.56WEE95 pKa = 4.13MIEE98 pKa = 4.66EE99 pKa = 4.21VFNTFEE105 pKa = 4.81AEE107 pKa = 4.09EE108 pKa = 4.82LEE110 pKa = 4.55DD111 pKa = 3.77

Molecular weight: 13.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X6WTK7|A0A1X6WTK7_9ENTE Chromosome (Plasmid) partitioning protein ParB OS=Vagococcus fluvialis bH819 OX=1255619 GN=FM121_12690 PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.7QPKK8 pKa = 8.83KK9 pKa = 9.05RR10 pKa = 11.84KK11 pKa = 7.43RR12 pKa = 11.84QKK14 pKa = 8.87VHH16 pKa = 5.8GFRR19 pKa = 11.84KK20 pKa = 10.04RR21 pKa = 11.84MSTKK25 pKa = 9.01NGRR28 pKa = 11.84RR29 pKa = 11.84VVASRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.58GRR39 pKa = 11.84KK40 pKa = 8.76VLAAA44 pKa = 4.31

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2822

0

2822

869045

37

4454

308.0

34.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.889 ± 0.056

0.616 ± 0.013

5.396 ± 0.043

7.707 ± 0.053

4.591 ± 0.037

6.251 ± 0.035

1.673 ± 0.02

8.344 ± 0.051

8.141 ± 0.051

9.619 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.784 ± 0.031

5.328 ± 0.038

3.133 ± 0.022

3.637 ± 0.028

3.284 ± 0.031

6.451 ± 0.038

5.824 ± 0.04

6.751 ± 0.036

0.842 ± 0.014

3.74 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski