Amino acid dipepetide frequency for Vagococcus fluvialis bH819

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.82AlaAla: 3.82 ± 0.095
0.484AlaCys: 0.484 ± 0.022
3.022AlaAsp: 3.022 ± 0.067
4.002AlaGlu: 4.002 ± 0.075
2.774AlaPhe: 2.774 ± 0.061
3.89AlaGly: 3.89 ± 0.084
0.969AlaHis: 0.969 ± 0.031
5.225AlaIle: 5.225 ± 0.095
4.783AlaLys: 4.783 ± 0.118
5.648AlaLeu: 5.648 ± 0.092
1.767AlaMet: 1.767 ± 0.044
2.888AlaAsn: 2.888 ± 0.058
1.705AlaPro: 1.705 ± 0.054
1.916AlaGln: 1.916 ± 0.051
1.946AlaArg: 1.946 ± 0.05
3.79AlaSer: 3.79 ± 0.073
3.602AlaThr: 3.602 ± 0.077
4.148AlaVal: 4.148 ± 0.082
0.433AlaTrp: 0.433 ± 0.023
2.072AlaTyr: 2.072 ± 0.05
0.0AlaXaa: 0.0 ± 0.0
Cys
0.31CysAla: 0.31 ± 0.019
0.075CysCys: 0.075 ± 0.012
0.362CysAsp: 0.362 ± 0.02
0.403CysGlu: 0.403 ± 0.022
0.318CysPhe: 0.318 ± 0.021
0.524CysGly: 0.524 ± 0.026
0.198CysHis: 0.198 ± 0.017
0.43CysIle: 0.43 ± 0.027
0.236CysLys: 0.236 ± 0.017
0.78CysLeu: 0.78 ± 0.033
0.146CysMet: 0.146 ± 0.013
0.183CysAsn: 0.183 ± 0.013
0.239CysPro: 0.239 ± 0.018
0.296CysGln: 0.296 ± 0.018
0.219CysArg: 0.219 ± 0.018
0.425CysSer: 0.425 ± 0.023
0.278CysThr: 0.278 ± 0.017
0.428CysVal: 0.428 ± 0.021
0.044CysTrp: 0.044 ± 0.007
0.272CysTyr: 0.272 ± 0.018
0.0CysXaa: 0.0 ± 0.0
Asp
2.962AspAla: 2.962 ± 0.075
0.352AspCys: 0.352 ± 0.021
2.769AspAsp: 2.769 ± 0.069
4.321AspGlu: 4.321 ± 0.094
2.805AspPhe: 2.805 ± 0.059
3.292AspGly: 3.292 ± 0.065
0.858AspHis: 0.858 ± 0.033
4.626AspIle: 4.626 ± 0.08
4.184AspLys: 4.184 ± 0.079
5.231AspLeu: 5.231 ± 0.073
1.476AspMet: 1.476 ± 0.046
2.752AspAsn: 2.752 ± 0.061
1.586AspPro: 1.586 ± 0.043
1.915AspGln: 1.915 ± 0.048
1.77AspArg: 1.77 ± 0.048
3.155AspSer: 3.155 ± 0.064
2.798AspThr: 2.798 ± 0.063
3.855AspVal: 3.855 ± 0.065
0.573AspTrp: 0.573 ± 0.025
2.682AspTyr: 2.682 ± 0.071
0.0AspXaa: 0.0 ± 0.0
Glu
4.966GluAla: 4.966 ± 0.104
0.352GluCys: 0.352 ± 0.023
3.815GluAsp: 3.815 ± 0.067
6.91GluGlu: 6.91 ± 0.121
2.808GluPhe: 2.808 ± 0.063
3.905GluGly: 3.905 ± 0.074
1.133GluHis: 1.133 ± 0.035
6.406GluIle: 6.406 ± 0.09
7.948GluLys: 7.948 ± 0.114
7.714GluLeu: 7.714 ± 0.107
2.5GluMet: 2.5 ± 0.059
4.466GluAsn: 4.466 ± 0.072
1.741GluPro: 1.741 ± 0.05
2.856GluGln: 2.856 ± 0.069
2.644GluArg: 2.644 ± 0.062
4.01GluSer: 4.01 ± 0.077
4.655GluThr: 4.655 ± 0.076
5.262GluVal: 5.262 ± 0.097
0.704GluTrp: 0.704 ± 0.031
2.276GluTyr: 2.276 ± 0.05
0.0GluXaa: 0.0 ± 0.0
Phe
2.254PheAla: 2.254 ± 0.062
0.323PheCys: 0.323 ± 0.019
2.781PheAsp: 2.781 ± 0.056
3.12PheGlu: 3.12 ± 0.068
2.479PhePhe: 2.479 ± 0.066
3.044PheGly: 3.044 ± 0.069
0.666PheHis: 0.666 ± 0.027
4.068PheIle: 4.068 ± 0.086
3.274PheLys: 3.274 ± 0.068
4.459PheLeu: 4.459 ± 0.1
1.243PheMet: 1.243 ± 0.036
2.634PheAsn: 2.634 ± 0.05
1.427PhePro: 1.427 ± 0.037
1.288PheGln: 1.288 ± 0.043
1.3PheArg: 1.3 ± 0.04
3.805PheSer: 3.805 ± 0.084
2.404PheThr: 2.404 ± 0.059
3.0PheVal: 3.0 ± 0.065
0.422PheTrp: 0.422 ± 0.027
1.917PheTyr: 1.917 ± 0.05
0.0PheXaa: 0.0 ± 0.0
Gly
3.923GlyAla: 3.923 ± 0.084
0.441GlyCys: 0.441 ± 0.024
3.029GlyAsp: 3.029 ± 0.063
4.037GlyGlu: 4.037 ± 0.088
3.109GlyPhe: 3.109 ± 0.066
4.136GlyGly: 4.136 ± 0.091
1.114GlyHis: 1.114 ± 0.038
5.778GlyIle: 5.778 ± 0.096
4.971GlyLys: 4.971 ± 0.08
6.026GlyLeu: 6.026 ± 0.101
1.978GlyMet: 1.978 ± 0.063
3.026GlyAsn: 3.026 ± 0.069
1.331GlyPro: 1.331 ± 0.035
2.109GlyGln: 2.109 ± 0.045
2.087GlyArg: 2.087 ± 0.063
3.968GlySer: 3.968 ± 0.078
3.805GlyThr: 3.805 ± 0.073
4.414GlyVal: 4.414 ± 0.076
0.548GlyTrp: 0.548 ± 0.028
2.679GlyTyr: 2.679 ± 0.064
0.0GlyXaa: 0.0 ± 0.0
His
0.912HisAla: 0.912 ± 0.036
0.125HisCys: 0.125 ± 0.013
0.841HisAsp: 0.841 ± 0.034
1.077HisGlu: 1.077 ± 0.036
0.982HisPhe: 0.982 ± 0.035
1.133HisGly: 1.133 ± 0.035
0.474HisHis: 0.474 ± 0.026
1.169HisIle: 1.169 ± 0.037
0.891HisLys: 0.891 ± 0.034
1.789HisLeu: 1.789 ± 0.048
0.387HisMet: 0.387 ± 0.024
0.636HisAsn: 0.636 ± 0.027
0.784HisPro: 0.784 ± 0.032
0.808HisGln: 0.808 ± 0.032
0.675HisArg: 0.675 ± 0.03
1.16HisSer: 1.16 ± 0.048
0.866HisThr: 0.866 ± 0.034
1.069HisVal: 1.069 ± 0.034
0.13HisTrp: 0.13 ± 0.013
0.819HisTyr: 0.819 ± 0.037
0.0HisXaa: 0.0 ± 0.0
Ile
5.239IleAla: 5.239 ± 0.092
0.612IleCys: 0.612 ± 0.028
4.717IleAsp: 4.717 ± 0.083
6.188IleGlu: 6.188 ± 0.085
3.907IlePhe: 3.907 ± 0.087
5.631IleGly: 5.631 ± 0.096
1.342IleHis: 1.342 ± 0.043
7.053IleIle: 7.053 ± 0.127
6.305IleLys: 6.305 ± 0.08
7.852IleLeu: 7.852 ± 0.123
2.072IleMet: 2.072 ± 0.044
4.536IleAsn: 4.536 ± 0.07
3.184IlePro: 3.184 ± 0.059
2.838IleGln: 2.838 ± 0.059
2.616IleArg: 2.616 ± 0.056
5.908IleSer: 5.908 ± 0.087
4.64IleThr: 4.64 ± 0.084
5.47IleVal: 5.47 ± 0.086
0.58IleTrp: 0.58 ± 0.028
2.747IleTyr: 2.747 ± 0.066
0.0IleXaa: 0.0 ± 0.0
Lys
4.365LysAla: 4.365 ± 0.099
0.296LysCys: 0.296 ± 0.019
4.686LysAsp: 4.686 ± 0.08
9.081LysGlu: 9.081 ± 0.118
2.347LysPhe: 2.347 ± 0.052
4.317LysGly: 4.317 ± 0.069
1.173LysHis: 1.173 ± 0.043
6.575LysIle: 6.575 ± 0.094
8.672LysLys: 8.672 ± 0.127
6.338LysLeu: 6.338 ± 0.089
2.66LysMet: 2.66 ± 0.054
5.366LysAsn: 5.366 ± 0.095
2.084LysPro: 2.084 ± 0.053
3.384LysGln: 3.384 ± 0.055
2.941LysArg: 2.941 ± 0.055
4.237LysSer: 4.237 ± 0.073
4.386LysThr: 4.386 ± 0.075
4.963LysVal: 4.963 ± 0.082
0.749LysTrp: 0.749 ± 0.033
2.787LysTyr: 2.787 ± 0.079
0.0LysXaa: 0.0 ± 0.0
Leu
6.208LeuAla: 6.208 ± 0.112
0.581LeuCys: 0.581 ± 0.024
5.313LeuAsp: 5.313 ± 0.075
6.984LeuGlu: 6.984 ± 0.117
4.749LeuPhe: 4.749 ± 0.093
6.014LeuGly: 6.014 ± 0.097
1.328LeuHis: 1.328 ± 0.041
7.933LeuIle: 7.933 ± 0.138
7.458LeuLys: 7.458 ± 0.093
9.468LeuLeu: 9.468 ± 0.147
2.557LeuMet: 2.557 ± 0.061
5.108LeuAsn: 5.108 ± 0.072
3.609LeuPro: 3.609 ± 0.074
2.655LeuGln: 2.655 ± 0.052
2.83LeuArg: 2.83 ± 0.07
7.248LeuSer: 7.248 ± 0.111
6.102LeuThr: 6.102 ± 0.099
6.455LeuVal: 6.455 ± 0.085
0.637LeuTrp: 0.637 ± 0.029
2.956LeuTyr: 2.956 ± 0.066
0.0LeuXaa: 0.0 ± 0.0
Met
1.864MetAla: 1.864 ± 0.051
0.13MetCys: 0.13 ± 0.013
1.428MetAsp: 1.428 ± 0.043
1.771MetGlu: 1.771 ± 0.051
1.068MetPhe: 1.068 ± 0.035
1.711MetGly: 1.711 ± 0.053
0.341MetHis: 0.341 ± 0.02
2.587MetIle: 2.587 ± 0.051
2.613MetLys: 2.613 ± 0.052
2.481MetLeu: 2.481 ± 0.06
0.952MetMet: 0.952 ± 0.039
1.634MetAsn: 1.634 ± 0.043
0.942MetPro: 0.942 ± 0.036
0.677MetGln: 0.677 ± 0.028
0.861MetArg: 0.861 ± 0.034
1.956MetSer: 1.956 ± 0.051
2.14MetThr: 2.14 ± 0.049
1.767MetVal: 1.767 ± 0.05
0.159MetTrp: 0.159 ± 0.013
0.763MetTyr: 0.763 ± 0.027
0.0MetXaa: 0.0 ± 0.0
Asn
2.643AsnAla: 2.643 ± 0.058
0.315AsnCys: 0.315 ± 0.02
2.672AsnAsp: 2.672 ± 0.061
4.058AsnGlu: 4.058 ± 0.072
2.413AsnPhe: 2.413 ± 0.055
3.315AsnGly: 3.315 ± 0.075
1.143AsnHis: 1.143 ± 0.04
4.165AsnIle: 4.165 ± 0.083
4.413AsnLys: 4.413 ± 0.089
4.858AsnLeu: 4.858 ± 0.085
1.377AsnMet: 1.377 ± 0.037
3.126AsnAsn: 3.126 ± 0.083
2.056AsnPro: 2.056 ± 0.048
2.964AsnGln: 2.964 ± 0.062
1.925AsnArg: 1.925 ± 0.053
3.01AsnSer: 3.01 ± 0.059
2.663AsnThr: 2.663 ± 0.073
3.205AsnVal: 3.205 ± 0.054
0.585AsnTrp: 0.585 ± 0.03
2.373AsnTyr: 2.373 ± 0.055
0.0AsnXaa: 0.0 ± 0.0
Pro
1.526ProAla: 1.526 ± 0.05
0.175ProCys: 0.175 ± 0.016
1.717ProAsp: 1.717 ± 0.046
2.78ProGlu: 2.78 ± 0.058
1.726ProPhe: 1.726 ± 0.051
1.787ProGly: 1.787 ± 0.059
0.532ProHis: 0.532 ± 0.024
2.728ProIle: 2.728 ± 0.053
2.511ProLys: 2.511 ± 0.051
2.872ProLeu: 2.872 ± 0.058
0.786ProMet: 0.786 ± 0.031
1.805ProAsn: 1.805 ± 0.042
0.586ProPro: 0.586 ± 0.029
1.0ProGln: 1.0 ± 0.033
0.833ProArg: 0.833 ± 0.03
1.973ProSer: 1.973 ± 0.052
2.045ProThr: 2.045 ± 0.051
2.449ProVal: 2.449 ± 0.053
0.244ProTrp: 0.244 ± 0.017
1.252ProTyr: 1.252 ± 0.038
0.0ProXaa: 0.0 ± 0.0
Gln
2.406GlnAla: 2.406 ± 0.065
0.139GlnCys: 0.139 ± 0.014
1.639GlnAsp: 1.639 ± 0.039
3.045GlnGlu: 3.045 ± 0.066
1.457GlnPhe: 1.457 ± 0.042
2.037GlnGly: 2.037 ± 0.052
0.639GlnHis: 0.639 ± 0.033
2.756GlnIle: 2.756 ± 0.056
3.206GlnLys: 3.206 ± 0.061
4.019GlnLeu: 4.019 ± 0.078
1.052GlnMet: 1.052 ± 0.032
1.772GlnAsn: 1.772 ± 0.045
1.077GlnPro: 1.077 ± 0.035
1.522GlnGln: 1.522 ± 0.049
1.22GlnArg: 1.22 ± 0.041
2.186GlnSer: 2.186 ± 0.048
2.179GlnThr: 2.179 ± 0.051
2.488GlnVal: 2.488 ± 0.057
0.303GlnTrp: 0.303 ± 0.02
1.228GlnTyr: 1.228 ± 0.041
0.0GlnXaa: 0.0 ± 0.0
Arg
1.675ArgAla: 1.675 ± 0.046
0.203ArgCys: 0.203 ± 0.015
1.704ArgAsp: 1.704 ± 0.046
2.688ArgGlu: 2.688 ± 0.055
1.472ArgPhe: 1.472 ± 0.045
1.922ArgGly: 1.922 ± 0.056
0.616ArgHis: 0.616 ± 0.028
2.655ArgIle: 2.655 ± 0.059
2.766ArgLys: 2.766 ± 0.057
3.276ArgLeu: 3.276 ± 0.06
1.003ArgMet: 1.003 ± 0.039
1.694ArgAsn: 1.694 ± 0.039
1.0ArgPro: 1.0 ± 0.053
1.422ArgGln: 1.422 ± 0.045
1.379ArgArg: 1.379 ± 0.047
1.754ArgSer: 1.754 ± 0.048
1.634ArgThr: 1.634 ± 0.048
2.312ArgVal: 2.312 ± 0.054
0.278ArgTrp: 0.278 ± 0.018
1.385ArgTyr: 1.385 ± 0.051
0.0ArgXaa: 0.0 ± 0.0
Ser
3.354SerAla: 3.354 ± 0.064
0.368SerCys: 0.368 ± 0.023
3.644SerAsp: 3.644 ± 0.071
4.732SerGlu: 4.732 ± 0.075
3.441SerPhe: 3.441 ± 0.072
4.561SerGly: 4.561 ± 0.084
1.224SerHis: 1.224 ± 0.042
5.239SerIle: 5.239 ± 0.083
4.9SerLys: 4.9 ± 0.073
6.642SerLeu: 6.642 ± 0.098
1.626SerMet: 1.626 ± 0.048
3.208SerAsn: 3.208 ± 0.07
1.8SerPro: 1.8 ± 0.045
2.445SerGln: 2.445 ± 0.057
2.059SerArg: 2.059 ± 0.053
4.284SerSer: 4.284 ± 0.108
3.466SerThr: 3.466 ± 0.081
4.35SerVal: 4.35 ± 0.074
0.596SerTrp: 0.596 ± 0.028
2.576SerTyr: 2.576 ± 0.067
0.0SerXaa: 0.0 ± 0.0
Thr
3.412ThrAla: 3.412 ± 0.073
0.333ThrCys: 0.333 ± 0.02
3.222ThrAsp: 3.222 ± 0.066
4.069ThrGlu: 4.069 ± 0.086
2.704ThrPhe: 2.704 ± 0.054
4.049ThrGly: 4.049 ± 0.077
1.046ThrHis: 1.046 ± 0.036
5.107ThrIle: 5.107 ± 0.08
4.449ThrLys: 4.449 ± 0.088
5.46ThrLeu: 5.46 ± 0.1
1.389ThrMet: 1.389 ± 0.036
2.983ThrAsn: 2.983 ± 0.066
2.236ThrPro: 2.236 ± 0.049
1.911ThrGln: 1.911 ± 0.048
1.692ThrArg: 1.692 ± 0.044
3.725ThrSer: 3.725 ± 0.073
3.676ThrThr: 3.676 ± 0.08
4.034ThrVal: 4.034 ± 0.078
0.51ThrTrp: 0.51 ± 0.025
2.237ThrTyr: 2.237 ± 0.064
0.0ThrXaa: 0.0 ± 0.0
Val
4.634ValAla: 4.634 ± 0.084
0.517ValCys: 0.517 ± 0.024
3.89ValAsp: 3.89 ± 0.078
4.628ValGlu: 4.628 ± 0.082
2.842ValPhe: 2.842 ± 0.055
4.408ValGly: 4.408 ± 0.087
0.941ValHis: 0.941 ± 0.033
5.836ValIle: 5.836 ± 0.103
4.926ValLys: 4.926 ± 0.088
6.173ValLeu: 6.173 ± 0.091
1.809ValMet: 1.809 ± 0.05
3.32ValAsn: 3.32 ± 0.06
2.396ValPro: 2.396 ± 0.057
1.786ValGln: 1.786 ± 0.049
2.093ValArg: 2.093 ± 0.052
4.971ValSer: 4.971 ± 0.079
4.458ValThr: 4.458 ± 0.069
5.018ValVal: 5.018 ± 0.087
0.513ValTrp: 0.513 ± 0.026
2.346ValTyr: 2.346 ± 0.06
0.0ValXaa: 0.0 ± 0.0
Trp
0.407TrpAla: 0.407 ± 0.025
0.045TrpCys: 0.045 ± 0.007
0.461TrpAsp: 0.461 ± 0.028
0.619TrpGlu: 0.619 ± 0.032
0.419TrpPhe: 0.419 ± 0.027
0.481TrpGly: 0.481 ± 0.025
0.184TrpHis: 0.184 ± 0.015
0.602TrpIle: 0.602 ± 0.025
0.64TrpLys: 0.64 ± 0.032
1.022TrpLeu: 1.022 ± 0.039
0.245TrpMet: 0.245 ± 0.017
0.487TrpAsn: 0.487 ± 0.027
0.181TrpPro: 0.181 ± 0.016
0.503TrpGln: 0.503 ± 0.025
0.282TrpArg: 0.282 ± 0.018
0.513TrpSer: 0.513 ± 0.025
0.413TrpThr: 0.413 ± 0.025
0.504TrpVal: 0.504 ± 0.024
0.094TrpTrp: 0.094 ± 0.011
0.319TrpTyr: 0.319 ± 0.023
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.006TyrAla: 2.006 ± 0.049
0.298TyrCys: 0.298 ± 0.02
2.252TyrAsp: 2.252 ± 0.065
2.56TyrGlu: 2.56 ± 0.055
2.089TyrPhe: 2.089 ± 0.053
2.35TyrGly: 2.35 ± 0.056
0.809TyrHis: 0.809 ± 0.031
2.498TyrIle: 2.498 ± 0.062
2.265TyrLys: 2.265 ± 0.064
4.081TyrLeu: 4.081 ± 0.08
0.817TyrMet: 0.817 ± 0.035
1.656TyrAsn: 1.656 ± 0.051
1.364TyrPro: 1.364 ± 0.039
2.075TyrGln: 2.075 ± 0.069
1.467TyrArg: 1.467 ± 0.049
2.436TyrSer: 2.436 ± 0.059
2.031TyrThr: 2.031 ± 0.069
2.315TyrVal: 2.315 ± 0.049
0.319TyrTrp: 0.319 ± 0.021
1.711TyrTyr: 1.711 ± 0.062
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2822 proteins (869045 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski