Staphylococcus virus 29
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 75 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q4ZAY1|Q4ZAY1_9CAUD ORF055 OS=Staphylococcus virus 29 OX=320846 PE=4 SV=1
MM1 pKa = 7.38 EE2 pKa = 4.48 MMNNRR7 pKa = 11.84 EE8 pKa = 4.09 QIEE11 pKa = 3.98 QSVISASAYY20 pKa = 10.04 NGNDD24 pKa = 3.29 TEE26 pKa = 4.52 GLLKK30 pKa = 10.49 EE31 pKa = 4.44 IEE33 pKa = 4.25 DD34 pKa = 4.16 VYY36 pKa = 11.47 KK37 pKa = 10.57 KK38 pKa = 10.68 ARR40 pKa = 11.84 AFDD43 pKa = 4.71 EE44 pKa = 4.26 ILEE47 pKa = 4.21 GLPNAMQDD55 pKa = 3.61 ALKK58 pKa = 10.63 EE59 pKa = 4.3 DD60 pKa = 3.43 IGLDD64 pKa = 3.48 EE65 pKa = 5.32 AVGIMTGQVVYY76 pKa = 10.25 KK77 pKa = 10.48 YY78 pKa = 10.54 EE79 pKa = 4.02 EE80 pKa = 4.2 EE81 pKa = 4.27 QEE83 pKa = 4.06 SDD85 pKa = 3.01
Molecular weight: 9.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.253
IPC2_protein 4.012
IPC_protein 3.923
Toseland 3.745
ProMoST 3.973
Dawson 3.872
Bjellqvist 4.075
Wikipedia 3.745
Rodwell 3.757
Grimsley 3.668
Solomon 3.859
Lehninger 3.808
Nozaki 3.986
DTASelect 4.113
Thurlkill 3.77
EMBOSS 3.77
Sillero 4.024
Patrickios 3.541
IPC_peptide 3.859
IPC2_peptide 4.012
IPC2.peptide.svr19 3.96
Protein with the highest isoelectric point:
>tr|Q4ZB36|Q4ZB36_9CAUD ORF050 OS=Staphylococcus virus 29 OX=320846 PE=4 SV=1
MM1 pKa = 7.74 LLILVVNTQYY11 pKa = 10.82 MSIMVLVYY19 pKa = 7.72 MQQVLEE25 pKa = 4.16 VVEE28 pKa = 4.42 RR29 pKa = 11.84 KK30 pKa = 9.5 RR31 pKa = 11.84 YY32 pKa = 8.92 HH33 pKa = 5.11 GHH35 pKa = 5.33 TRR37 pKa = 11.84 MQTVSGTLLKK47 pKa = 11.25 GNMHH51 pKa = 6.67 NLFGSRR57 pKa = 11.84 QQ58 pKa = 3.34
Molecular weight: 6.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.253
IPC2_protein 9.897
IPC_protein 10.496
Toseland 10.145
ProMoST 9.999
Dawson 10.409
Bjellqvist 10.16
Wikipedia 10.643
Rodwell 10.599
Grimsley 10.511
Solomon 10.482
Lehninger 10.438
Nozaki 10.101
DTASelect 10.16
Thurlkill 10.233
EMBOSS 10.57
Sillero 10.321
Patrickios 10.423
IPC_peptide 10.467
IPC2_peptide 9.033
IPC2.peptide.svr19 8.451
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
75
0
75
13864
33
1047
184.9
21.13
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.749 ± 0.351
0.57 ± 0.112
6.08 ± 0.278
7.321 ± 0.452
4.299 ± 0.229
5.814 ± 0.368
1.789 ± 0.148
7.473 ± 0.259
8.966 ± 0.347
7.761 ± 0.29
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.698 ± 0.177
6.268 ± 0.22
3.008 ± 0.262
4.018 ± 0.218
4.003 ± 0.234
5.792 ± 0.282
6.448 ± 0.266
6.391 ± 0.316
1.161 ± 0.179
4.393 ± 0.346
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here