Algoriphagus machipongonensis

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Cyclobacteriaceae; Algoriphagus

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3932 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A3HZS1|A3HZS1_9BACT Uncharacterized protein OS=Algoriphagus machipongonensis OX=388413 GN=ALPR1_07525 PE=4 SV=1
MM1 pKa = 7.59KK2 pKa = 10.68SFINKK7 pKa = 9.56LGLFGVVLLTLTSCLDD23 pKa = 3.74EE24 pKa = 6.21DD25 pKa = 4.97PLFDD29 pKa = 4.86PSNSTGIIEE38 pKa = 4.09IVEE41 pKa = 4.2IGPLATSGSIYY52 pKa = 10.33PMNRR56 pKa = 11.84LTFEE60 pKa = 4.6SVPEE64 pKa = 3.99DD65 pKa = 3.47QIEE68 pKa = 4.56VIVQYY73 pKa = 10.87SGAFDD78 pKa = 3.8APEE81 pKa = 5.3DD82 pKa = 3.56IDD84 pKa = 3.83VTIEE88 pKa = 4.01VAPSALDD95 pKa = 4.77DD96 pKa = 4.3FNADD100 pKa = 3.23QGLDD104 pKa = 3.58EE105 pKa = 4.99SNGYY109 pKa = 9.79YY110 pKa = 10.01IIDD113 pKa = 4.08DD114 pKa = 3.78SSYY117 pKa = 10.51EE118 pKa = 3.94LPGGGNSVTVTIPKK132 pKa = 9.51GEE134 pKa = 4.02KK135 pKa = 8.42RR136 pKa = 11.84VSVIVTVRR144 pKa = 11.84PDD146 pKa = 3.11QFGFDD151 pKa = 3.87KK152 pKa = 11.08NYY154 pKa = 10.76ALPIRR159 pKa = 11.84IASASSGQVSGNFSNMIYY177 pKa = 10.74AVIPNNQWAGDD188 pKa = 3.58WTNTYY193 pKa = 10.55SSPFGSGTNTVHH205 pKa = 6.8MSTTGEE211 pKa = 4.02FTTTSNLIGVYY222 pKa = 10.3SNQTVIEE229 pKa = 4.26VNPDD233 pKa = 2.93TNYY236 pKa = 10.99AQVLSVSGLGPVTNSPDD253 pKa = 3.71NYY255 pKa = 9.68WDD257 pKa = 4.38PEE259 pKa = 4.16TKK261 pKa = 9.83TIYY264 pKa = 10.63LSFVSSGYY272 pKa = 10.87AFEE275 pKa = 3.89QTMVKK280 pKa = 10.2KK281 pKa = 10.69

Molecular weight: 30.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A3HXH8|A3HXH8_9BACT Putative outer membrane protein OS=Algoriphagus machipongonensis OX=388413 GN=ALPR1_19733 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84RR10 pKa = 11.84KK11 pKa = 9.77RR12 pKa = 11.84KK13 pKa = 8.25NKK15 pKa = 9.37HH16 pKa = 4.12GFRR19 pKa = 11.84EE20 pKa = 4.21RR21 pKa = 11.84MSSANGRR28 pKa = 11.84RR29 pKa = 11.84VIKK32 pKa = 10.36ARR34 pKa = 11.84RR35 pKa = 11.84SKK37 pKa = 10.55GRR39 pKa = 11.84HH40 pKa = 5.05KK41 pKa = 10.93LSVSSEE47 pKa = 3.97KK48 pKa = 9.91TLKK51 pKa = 10.51KK52 pKa = 10.72

Molecular weight: 6.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3932

0

3932

1430355

38

9449

363.8

40.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.438 ± 0.036

0.638 ± 0.013

5.572 ± 0.033

7.221 ± 0.045

5.263 ± 0.035

7.166 ± 0.045

1.733 ± 0.022

7.26 ± 0.03

6.852 ± 0.055

9.666 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.421 ± 0.025

5.201 ± 0.044

4.028 ± 0.023

3.646 ± 0.023

3.638 ± 0.031

6.872 ± 0.035

5.15 ± 0.07

6.17 ± 0.038

1.278 ± 0.016

3.788 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski