Rotavirus A pheasant-tc/GER/10V0112H5/2010/G23P[37]
Average proteome isoelectric point is 6.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|L0CSN4|L0CSN4_9REOV Non-structural protein 5 OS=Rotavirus A pheasant-tc/GER/10V0112H5/2010/G23P[37] OX=1271534 PE=4 SV=1
MM1 pKa = 7.49 YY2 pKa = 9.41 STEE5 pKa = 4.05 CTSFFLEE12 pKa = 4.15 IIFYY16 pKa = 10.44 VILYY20 pKa = 7.18 TIISSRR26 pKa = 11.84 LSRR29 pKa = 11.84 MSRR32 pKa = 11.84 LFYY35 pKa = 10.16 WIIIFIIFSSLCVSKK50 pKa = 10.92 CSSQNFGINVPITGSLDD67 pKa = 3.41 VTVLNSTQTQIGLTSTLCIYY87 pKa = 10.0 YY88 pKa = 9.69 PKK90 pKa = 10.12 EE91 pKa = 3.83 ASDD94 pKa = 4.14 EE95 pKa = 4.34 IKK97 pKa = 10.83 DD98 pKa = 4.09 SEE100 pKa = 4.32 WKK102 pKa = 8.72 NTVSQLLLTKK112 pKa = 9.96 GWPTTSVYY120 pKa = 10.89 LNEE123 pKa = 4.26 YY124 pKa = 9.77 NDD126 pKa = 4.06 LAAFSNDD133 pKa = 2.55 PKK135 pKa = 10.92 LYY137 pKa = 9.75 CDD139 pKa = 3.72 YY140 pKa = 11.28 NIVLVKK146 pKa = 9.17 YY147 pKa = 8.33 TGSNVALDD155 pKa = 3.47 ISEE158 pKa = 4.28 LAEE161 pKa = 3.99 LLLYY165 pKa = 10.18 EE166 pKa = 4.42 WLCNPMDD173 pKa = 3.53 VTLYY177 pKa = 10.62 YY178 pKa = 9.99 YY179 pKa = 10.53 QQTSEE184 pKa = 4.23 SNKK187 pKa = 8.2 WIAMGTDD194 pKa = 3.77 CTIKK198 pKa = 10.33 VCPLNVQTLGIGCQTTDD215 pKa = 2.68 VNTFEE220 pKa = 4.48 QVTASEE226 pKa = 4.0 QLAIIDD232 pKa = 3.76 VVDD235 pKa = 4.36 GVNHH239 pKa = 7.27 KK240 pKa = 10.36 INYY243 pKa = 7.41 TLTTCTIKK251 pKa = 10.89 NCIRR255 pKa = 11.84 LNPRR259 pKa = 11.84 GNVAIIQIGGPEE271 pKa = 3.96 ILDD274 pKa = 3.32 ISEE277 pKa = 4.74 DD278 pKa = 3.66 PMVVPKK284 pKa = 9.3 MVRR287 pKa = 11.84 MTRR290 pKa = 11.84 INWKK294 pKa = 9.41 KK295 pKa = 7.27 WWQVFYY301 pKa = 10.64 TIVDD305 pKa = 3.85 YY306 pKa = 11.35 INTIVNAMSKK316 pKa = 10.25 RR317 pKa = 11.84 ATSLDD322 pKa = 2.99 ASSYY326 pKa = 7.88 YY327 pKa = 10.84 LRR329 pKa = 11.84 VV330 pKa = 3.27
Molecular weight: 37.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.887
IPC2_protein 4.965
IPC_protein 4.863
Toseland 4.698
ProMoST 4.965
Dawson 4.813
Bjellqvist 4.965
Wikipedia 4.685
Rodwell 4.698
Grimsley 4.609
Solomon 4.813
Lehninger 4.762
Nozaki 4.914
DTASelect 5.092
Thurlkill 4.711
EMBOSS 4.711
Sillero 4.978
Patrickios 0.642
IPC_peptide 4.813
IPC2_peptide 4.965
IPC2.peptide.svr19 4.899
Protein with the highest isoelectric point:
>tr|L0CRY4|L0CRY4_9REOV Intermediate capsid protein VP6 OS=Rotavirus A pheasant-tc/GER/10V0112H5/2010/G23P[37] OX=1271534 PE=3 SV=1
MM1 pKa = 6.85 NHH3 pKa = 5.71 LVKK6 pKa = 10.67 RR7 pKa = 11.84 QVLQGNLLVGVSSMYY22 pKa = 10.41 RR23 pKa = 11.84 CPKK26 pKa = 9.39 KK27 pKa = 10.15 HH28 pKa = 6.4 LANTCLIGIQKK39 pKa = 8.46 TLDD42 pKa = 3.36 HH43 pKa = 7.23 LIQHH47 pKa = 6.18 QMTCPPNLLLNQMQLNQMLMLACRR71 pKa = 11.84 WMLDD75 pKa = 3.03 QWIVRR80 pKa = 11.84 QEE82 pKa = 3.96 TMQKK86 pKa = 8.57 MKK88 pKa = 10.79 SIFHH92 pKa = 5.94 SLKK95 pKa = 10.27 EE96 pKa = 3.95
Molecular weight: 11.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.0
IPC2_protein 8.902
IPC_protein 8.873
Toseland 9.853
ProMoST 9.399
Dawson 9.984
Bjellqvist 9.692
Wikipedia 10.072
Rodwell 10.613
Grimsley 9.984
Solomon 10.058
Lehninger 10.043
Nozaki 10.043
DTASelect 9.604
Thurlkill 9.882
EMBOSS 10.233
Sillero 9.97
Patrickios 10.496
IPC_peptide 10.058
IPC2_peptide 8.682
IPC2.peptide.svr19 7.794
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12
0
12
5986
96
1089
498.8
57.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.396 ± 0.352
1.286 ± 0.368
6.215 ± 0.231
5.379 ± 0.269
4.227 ± 0.199
3.375 ± 0.343
1.57 ± 0.356
7.685 ± 0.271
6.799 ± 0.525
9.422 ± 0.323
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.74 ± 0.318
6.866 ± 0.339
3.291 ± 0.316
4.059 ± 0.531
4.744 ± 0.319
7.785 ± 0.779
6.498 ± 0.388
6.365 ± 0.223
1.169 ± 0.172
5.129 ± 0.609
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here