Streptococcus phage Javan227

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6A3J4|A0A4D6A3J4_9CAUD Holliday junction resolvase OS=Streptococcus phage Javan227 OX=2548056 GN=Javan227_0021 PE=4 SV=1
MM1 pKa = 7.45NKK3 pKa = 9.44QEE5 pKa = 4.37IMSGMEE11 pKa = 4.6SIMQIAMCYY20 pKa = 8.57CHH22 pKa = 7.03PSVPDD27 pKa = 3.11VDD29 pKa = 4.53DD30 pKa = 3.23WHH32 pKa = 6.76AVHH35 pKa = 7.02SIAEE39 pKa = 4.34TIYY42 pKa = 11.16DD43 pKa = 3.94EE44 pKa = 4.44LEE46 pKa = 4.16KK47 pKa = 11.56GEE49 pKa = 4.53GEE51 pKa = 4.01VDD53 pKa = 3.45EE54 pKa = 4.79

Molecular weight: 6.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6A3L5|A0A4D6A3L5_9CAUD Uncharacterized protein OS=Streptococcus phage Javan227 OX=2548056 GN=Javan227_0013 PE=4 SV=1
MM1 pKa = 7.35LASALGLYY9 pKa = 10.29GVGSILEE16 pKa = 4.31AKK18 pKa = 10.41RR19 pKa = 11.84MTLEE23 pKa = 3.74EE24 pKa = 4.12FNVRR28 pKa = 11.84KK29 pKa = 9.78RR30 pKa = 11.84GYY32 pKa = 10.63LMQRR36 pKa = 11.84LEE38 pKa = 4.28RR39 pKa = 11.84EE40 pKa = 4.22RR41 pKa = 11.84EE42 pKa = 3.76IHH44 pKa = 6.03LQAYY48 pKa = 8.91LNRR51 pKa = 11.84LIKK54 pKa = 10.36ATDD57 pKa = 3.41KK58 pKa = 11.15SGKK61 pKa = 9.88NYY63 pKa = 10.65LYY65 pKa = 11.3GNFNDD70 pKa = 5.65FYY72 pKa = 11.55NEE74 pKa = 3.31AKK76 pKa = 10.51ARR78 pKa = 11.84NTVLGNGHH86 pKa = 6.57GEE88 pKa = 4.17PVSSDD93 pKa = 4.0LIAIAKK99 pKa = 9.52RR100 pKa = 11.84RR101 pKa = 11.84QKK103 pKa = 10.88LLRR106 pKa = 11.84KK107 pKa = 9.7GDD109 pKa = 3.79NNGG112 pKa = 3.08

Molecular weight: 12.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

12180

46

1458

221.5

25.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.02 ± 0.528

0.599 ± 0.108

6.256 ± 0.34

7.57 ± 0.585

4.015 ± 0.263

5.616 ± 0.432

1.412 ± 0.157

7.192 ± 0.323

8.054 ± 0.402

8.432 ± 0.347

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.094 ± 0.174

6.199 ± 0.333

2.521 ± 0.201

4.22 ± 0.272

3.818 ± 0.284

6.987 ± 0.508

6.355 ± 0.54

6.264 ± 0.276

1.141 ± 0.19

4.236 ± 0.299
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski