Amino acid dipepetide frequency for Streptococcus phage Javan227

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.105AlaAla: 4.105 ± 1.196
0.328AlaCys: 0.328 ± 0.151
4.27AlaAsp: 4.27 ± 0.634
5.255AlaGlu: 5.255 ± 0.834
2.217AlaPhe: 2.217 ± 0.315
4.598AlaGly: 4.598 ± 0.701
1.067AlaHis: 1.067 ± 0.293
5.173AlaIle: 5.173 ± 0.843
5.255AlaLys: 5.255 ± 0.631
5.501AlaLeu: 5.501 ± 0.655
1.724AlaMet: 1.724 ± 0.397
3.859AlaAsn: 3.859 ± 0.821
1.888AlaPro: 1.888 ± 0.387
4.105AlaGln: 4.105 ± 0.73
3.202AlaArg: 3.202 ± 0.554
5.337AlaSer: 5.337 ± 0.76
4.516AlaThr: 4.516 ± 0.564
4.27AlaVal: 4.27 ± 0.663
1.067AlaTrp: 1.067 ± 0.483
2.463AlaTyr: 2.463 ± 0.48
0.0AlaXaa: 0.0 ± 0.0
Cys
0.246CysAla: 0.246 ± 0.138
0.0CysCys: 0.0 ± 0.0
0.575CysAsp: 0.575 ± 0.221
0.328CysGlu: 0.328 ± 0.156
0.246CysPhe: 0.246 ± 0.146
0.575CysGly: 0.575 ± 0.242
0.082CysHis: 0.082 ± 0.082
0.328CysIle: 0.328 ± 0.157
0.411CysLys: 0.411 ± 0.162
0.246CysLeu: 0.246 ± 0.114
0.0CysMet: 0.0 ± 0.0
0.328CysAsn: 0.328 ± 0.217
0.082CysPro: 0.082 ± 0.075
0.246CysGln: 0.246 ± 0.146
0.246CysArg: 0.246 ± 0.145
0.575CysSer: 0.575 ± 0.186
0.246CysThr: 0.246 ± 0.148
0.328CysVal: 0.328 ± 0.151
0.328CysTrp: 0.328 ± 0.151
0.575CysTyr: 0.575 ± 0.221
0.0CysXaa: 0.0 ± 0.0
Asp
2.792AspAla: 2.792 ± 0.541
0.411AspCys: 0.411 ± 0.196
4.598AspAsp: 4.598 ± 0.606
4.68AspGlu: 4.68 ± 0.924
3.695AspPhe: 3.695 ± 0.625
4.105AspGly: 4.105 ± 0.748
0.246AspHis: 0.246 ± 0.156
3.284AspIle: 3.284 ± 0.456
4.105AspLys: 4.105 ± 0.629
5.912AspLeu: 5.912 ± 0.655
1.971AspMet: 1.971 ± 0.452
4.598AspAsn: 4.598 ± 0.66
1.232AspPro: 1.232 ± 0.31
1.724AspGln: 1.724 ± 0.387
1.724AspArg: 1.724 ± 0.351
4.68AspSer: 4.68 ± 0.598
4.27AspThr: 4.27 ± 0.579
3.777AspVal: 3.777 ± 0.66
0.985AspTrp: 0.985 ± 0.328
3.777AspTyr: 3.777 ± 0.551
0.0AspXaa: 0.0 ± 0.0
Glu
5.255GluAla: 5.255 ± 0.807
0.328GluCys: 0.328 ± 0.195
4.188GluAsp: 4.188 ± 0.663
5.091GluGlu: 5.091 ± 0.841
3.449GluPhe: 3.449 ± 0.566
3.366GluGly: 3.366 ± 0.569
1.56GluHis: 1.56 ± 0.342
6.815GluIle: 6.815 ± 0.89
5.83GluLys: 5.83 ± 0.87
9.771GluLeu: 9.771 ± 1.315
1.806GluMet: 1.806 ± 0.467
4.352GluAsn: 4.352 ± 0.494
1.971GluPro: 1.971 ± 0.415
2.627GluGln: 2.627 ± 0.555
2.874GluArg: 2.874 ± 0.507
2.956GluSer: 2.956 ± 0.483
4.188GluThr: 4.188 ± 0.477
5.009GluVal: 5.009 ± 0.777
0.985GluTrp: 0.985 ± 0.362
3.284GluTyr: 3.284 ± 0.627
0.0GluXaa: 0.0 ± 0.0
Phe
2.217PheAla: 2.217 ± 0.388
0.328PheCys: 0.328 ± 0.165
3.12PheAsp: 3.12 ± 0.525
4.188PheGlu: 4.188 ± 0.931
1.888PhePhe: 1.888 ± 0.426
2.217PheGly: 2.217 ± 0.513
0.739PheHis: 0.739 ± 0.284
2.463PheIle: 2.463 ± 0.537
3.366PheLys: 3.366 ± 0.408
3.366PheLeu: 3.366 ± 0.556
1.15PheMet: 1.15 ± 0.301
1.888PheAsn: 1.888 ± 0.462
0.985PhePro: 0.985 ± 0.342
1.067PheGln: 1.067 ± 0.261
1.724PheArg: 1.724 ± 0.361
2.545PheSer: 2.545 ± 0.466
1.971PheThr: 1.971 ± 0.315
2.874PheVal: 2.874 ± 0.436
0.411PheTrp: 0.411 ± 0.174
1.642PheTyr: 1.642 ± 0.435
0.0PheXaa: 0.0 ± 0.0
Gly
4.023GlyAla: 4.023 ± 0.752
0.328GlyCys: 0.328 ± 0.129
3.531GlyAsp: 3.531 ± 0.478
3.449GlyGlu: 3.449 ± 0.477
2.299GlyPhe: 2.299 ± 0.472
3.12GlyGly: 3.12 ± 0.726
0.575GlyHis: 0.575 ± 0.221
5.501GlyIle: 5.501 ± 0.97
4.927GlyLys: 4.927 ± 0.604
5.665GlyLeu: 5.665 ± 0.885
0.821GlyMet: 0.821 ± 0.309
3.202GlyAsn: 3.202 ± 0.459
0.246GlyPro: 0.246 ± 0.216
2.463GlyGln: 2.463 ± 0.463
2.135GlyArg: 2.135 ± 0.463
4.105GlySer: 4.105 ± 0.565
3.12GlyThr: 3.12 ± 0.72
3.202GlyVal: 3.202 ± 0.52
0.575GlyTrp: 0.575 ± 0.27
2.874GlyTyr: 2.874 ± 0.42
0.0GlyXaa: 0.0 ± 0.0
His
0.903HisAla: 0.903 ± 0.282
0.164HisCys: 0.164 ± 0.151
0.739HisAsp: 0.739 ± 0.261
0.903HisGlu: 0.903 ± 0.352
0.903HisPhe: 0.903 ± 0.269
0.985HisGly: 0.985 ± 0.327
0.493HisHis: 0.493 ± 0.215
0.821HisIle: 0.821 ± 0.275
1.396HisLys: 1.396 ± 0.359
1.15HisLeu: 1.15 ± 0.294
0.246HisMet: 0.246 ± 0.193
0.493HisAsn: 0.493 ± 0.201
0.328HisPro: 0.328 ± 0.146
0.493HisGln: 0.493 ± 0.212
0.411HisArg: 0.411 ± 0.175
1.15HisSer: 1.15 ± 0.314
0.739HisThr: 0.739 ± 0.241
1.232HisVal: 1.232 ± 0.34
0.082HisTrp: 0.082 ± 0.088
0.493HisTyr: 0.493 ± 0.156
0.0HisXaa: 0.0 ± 0.0
Ile
6.815IleAla: 6.815 ± 0.746
0.493IleCys: 0.493 ± 0.147
4.516IleAsp: 4.516 ± 0.591
5.583IleGlu: 5.583 ± 0.808
2.463IlePhe: 2.463 ± 0.506
3.613IleGly: 3.613 ± 0.593
0.821IleHis: 0.821 ± 0.269
4.68IleIle: 4.68 ± 0.786
7.39IleLys: 7.39 ± 0.78
5.091IleLeu: 5.091 ± 0.781
1.232IleMet: 1.232 ± 0.385
5.912IleAsn: 5.912 ± 0.678
1.971IlePro: 1.971 ± 0.31
1.806IleGln: 1.806 ± 0.296
1.642IleArg: 1.642 ± 0.337
5.419IleSer: 5.419 ± 0.692
4.188IleThr: 4.188 ± 0.9
5.091IleVal: 5.091 ± 0.759
0.903IleTrp: 0.903 ± 0.261
2.217IleTyr: 2.217 ± 0.524
0.0IleXaa: 0.0 ± 0.0
Lys
5.665LysAla: 5.665 ± 0.77
0.575LysCys: 0.575 ± 0.226
4.188LysAsp: 4.188 ± 0.571
7.39LysGlu: 7.39 ± 0.961
2.792LysPhe: 2.792 ± 0.481
3.695LysGly: 3.695 ± 0.476
0.903LysHis: 0.903 ± 0.286
5.83LysIle: 5.83 ± 0.619
5.665LysLys: 5.665 ± 0.775
7.061LysLeu: 7.061 ± 0.867
1.56LysMet: 1.56 ± 0.389
4.762LysAsn: 4.762 ± 0.667
1.888LysPro: 1.888 ± 0.366
4.105LysGln: 4.105 ± 0.54
4.598LysArg: 4.598 ± 0.876
4.927LysSer: 4.927 ± 0.593
5.091LysThr: 5.091 ± 0.569
5.173LysVal: 5.173 ± 0.575
0.903LysTrp: 0.903 ± 0.309
3.777LysTyr: 3.777 ± 0.582
0.0LysXaa: 0.0 ± 0.0
Leu
6.158LeuAla: 6.158 ± 0.815
0.164LeuCys: 0.164 ± 0.117
6.569LeuAsp: 6.569 ± 0.742
7.143LeuGlu: 7.143 ± 1.009
1.971LeuPhe: 1.971 ± 0.429
4.434LeuGly: 4.434 ± 0.712
1.396LeuHis: 1.396 ± 0.331
5.501LeuIle: 5.501 ± 0.999
8.868LeuLys: 8.868 ± 1.021
6.979LeuLeu: 6.979 ± 1.068
1.314LeuMet: 1.314 ± 0.414
5.748LeuAsn: 5.748 ± 0.892
2.463LeuPro: 2.463 ± 0.452
3.366LeuGln: 3.366 ± 0.544
3.038LeuArg: 3.038 ± 0.495
6.897LeuSer: 6.897 ± 0.907
4.68LeuThr: 4.68 ± 0.679
4.188LeuVal: 4.188 ± 0.637
0.657LeuTrp: 0.657 ± 0.31
2.792LeuTyr: 2.792 ± 0.575
0.0LeuXaa: 0.0 ± 0.0
Met
1.642MetAla: 1.642 ± 0.452
0.082MetCys: 0.082 ± 0.082
1.15MetAsp: 1.15 ± 0.331
1.15MetGlu: 1.15 ± 0.288
0.821MetPhe: 0.821 ± 0.23
0.985MetGly: 0.985 ± 0.348
0.328MetHis: 0.328 ± 0.179
1.396MetIle: 1.396 ± 0.322
1.724MetLys: 1.724 ± 0.316
1.56MetLeu: 1.56 ± 0.322
0.575MetMet: 0.575 ± 0.227
1.15MetAsn: 1.15 ± 0.239
0.821MetPro: 0.821 ± 0.207
1.478MetGln: 1.478 ± 0.276
1.232MetArg: 1.232 ± 0.329
1.15MetSer: 1.15 ± 0.296
2.217MetThr: 2.217 ± 0.49
0.657MetVal: 0.657 ± 0.225
0.164MetTrp: 0.164 ± 0.101
0.657MetTyr: 0.657 ± 0.241
0.0MetXaa: 0.0 ± 0.0
Asn
3.695AsnAla: 3.695 ± 0.618
0.328AsnCys: 0.328 ± 0.153
2.956AsnAsp: 2.956 ± 0.62
3.941AsnGlu: 3.941 ± 0.802
2.381AsnPhe: 2.381 ± 0.395
5.83AsnGly: 5.83 ± 0.62
0.903AsnHis: 0.903 ± 0.282
4.023AsnIle: 4.023 ± 0.592
3.941AsnLys: 3.941 ± 0.45
4.598AsnLeu: 4.598 ± 0.798
0.903AsnMet: 0.903 ± 0.342
4.105AsnAsn: 4.105 ± 0.703
2.627AsnPro: 2.627 ± 0.471
2.545AsnGln: 2.545 ± 0.462
2.217AsnArg: 2.217 ± 0.529
4.844AsnSer: 4.844 ± 0.929
4.023AsnThr: 4.023 ± 0.604
3.695AsnVal: 3.695 ± 0.527
0.985AsnTrp: 0.985 ± 0.315
3.449AsnTyr: 3.449 ± 0.7
0.0AsnXaa: 0.0 ± 0.0
Pro
1.724ProAla: 1.724 ± 0.363
0.082ProCys: 0.082 ± 0.093
2.135ProAsp: 2.135 ± 0.425
2.381ProGlu: 2.381 ± 0.482
1.314ProPhe: 1.314 ± 0.346
0.493ProGly: 0.493 ± 0.209
0.411ProHis: 0.411 ± 0.213
2.053ProIle: 2.053 ± 0.433
1.888ProLys: 1.888 ± 0.523
1.15ProLeu: 1.15 ± 0.268
0.493ProMet: 0.493 ± 0.189
1.971ProAsn: 1.971 ± 0.574
0.328ProPro: 0.328 ± 0.186
1.15ProGln: 1.15 ± 0.317
1.15ProArg: 1.15 ± 0.422
1.806ProSer: 1.806 ± 0.257
1.888ProThr: 1.888 ± 0.345
1.642ProVal: 1.642 ± 0.301
0.246ProTrp: 0.246 ± 0.121
0.903ProTyr: 0.903 ± 0.21
0.0ProXaa: 0.0 ± 0.0
Gln
3.449GlnAla: 3.449 ± 0.696
0.411GlnCys: 0.411 ± 0.191
1.642GlnAsp: 1.642 ± 0.329
3.202GlnGlu: 3.202 ± 0.879
1.642GlnPhe: 1.642 ± 0.342
1.888GlnGly: 1.888 ± 0.481
0.739GlnHis: 0.739 ± 0.27
3.202GlnIle: 3.202 ± 0.561
3.531GlnLys: 3.531 ± 0.792
2.792GlnLeu: 2.792 ± 0.424
0.328GlnMet: 0.328 ± 0.154
3.038GlnAsn: 3.038 ± 0.575
0.985GlnPro: 0.985 ± 0.396
2.956GlnGln: 2.956 ± 0.571
1.806GlnArg: 1.806 ± 0.415
2.956GlnSer: 2.956 ± 0.459
3.366GlnThr: 3.366 ± 0.606
2.792GlnVal: 2.792 ± 0.755
0.164GlnTrp: 0.164 ± 0.12
1.314GlnTyr: 1.314 ± 0.334
0.0GlnXaa: 0.0 ± 0.0
Arg
3.12ArgAla: 3.12 ± 0.637
0.246ArgCys: 0.246 ± 0.15
1.56ArgAsp: 1.56 ± 0.31
3.038ArgGlu: 3.038 ± 0.584
1.724ArgPhe: 1.724 ± 0.381
1.15ArgGly: 1.15 ± 0.324
0.657ArgHis: 0.657 ± 0.189
3.12ArgIle: 3.12 ± 0.545
3.613ArgLys: 3.613 ± 0.662
3.366ArgLeu: 3.366 ± 0.46
1.56ArgMet: 1.56 ± 0.313
2.381ArgAsn: 2.381 ± 0.447
0.657ArgPro: 0.657 ± 0.211
2.217ArgGln: 2.217 ± 0.525
1.642ArgArg: 1.642 ± 0.516
1.56ArgSer: 1.56 ± 0.312
1.888ArgThr: 1.888 ± 0.393
2.217ArgVal: 2.217 ± 0.46
0.164ArgTrp: 0.164 ± 0.104
2.299ArgTyr: 2.299 ± 0.534
0.0ArgXaa: 0.0 ± 0.0
Ser
5.337SerAla: 5.337 ± 1.061
0.246SerCys: 0.246 ± 0.147
5.501SerAsp: 5.501 ± 0.754
5.994SerGlu: 5.994 ± 0.639
2.71SerPhe: 2.71 ± 0.539
4.762SerGly: 4.762 ± 0.593
0.821SerHis: 0.821 ± 0.23
5.009SerIle: 5.009 ± 0.831
4.762SerLys: 4.762 ± 0.7
5.748SerLeu: 5.748 ± 0.701
1.724SerMet: 1.724 ± 0.358
4.188SerAsn: 4.188 ± 0.824
1.642SerPro: 1.642 ± 0.404
3.038SerGln: 3.038 ± 0.594
2.627SerArg: 2.627 ± 0.449
4.434SerSer: 4.434 ± 0.694
4.844SerThr: 4.844 ± 0.909
3.695SerVal: 3.695 ± 0.752
0.411SerTrp: 0.411 ± 0.235
2.381SerTyr: 2.381 ± 0.52
0.0SerXaa: 0.0 ± 0.0
Thr
5.748ThrAla: 5.748 ± 1.325
0.493ThrCys: 0.493 ± 0.214
3.202ThrAsp: 3.202 ± 0.472
4.27ThrGlu: 4.27 ± 0.743
3.366ThrPhe: 3.366 ± 0.455
3.695ThrGly: 3.695 ± 0.579
0.821ThrHis: 0.821 ± 0.286
4.68ThrIle: 4.68 ± 0.655
5.009ThrLys: 5.009 ± 0.787
5.83ThrLeu: 5.83 ± 0.601
0.657ThrMet: 0.657 ± 0.24
2.956ThrAsn: 2.956 ± 0.518
2.299ThrPro: 2.299 ± 0.644
2.381ThrGln: 2.381 ± 0.44
1.478ThrArg: 1.478 ± 0.371
4.434ThrSer: 4.434 ± 0.908
3.941ThrThr: 3.941 ± 0.764
5.173ThrVal: 5.173 ± 0.684
0.411ThrTrp: 0.411 ± 0.15
2.71ThrTyr: 2.71 ± 0.533
0.0ThrXaa: 0.0 ± 0.0
Val
4.434ValAla: 4.434 ± 0.726
0.411ValCys: 0.411 ± 0.227
3.859ValAsp: 3.859 ± 0.751
4.105ValGlu: 4.105 ± 0.762
2.135ValPhe: 2.135 ± 0.452
3.777ValGly: 3.777 ± 0.638
0.985ValHis: 0.985 ± 0.26
4.023ValIle: 4.023 ± 0.652
5.419ValLys: 5.419 ± 0.738
4.434ValLeu: 4.434 ± 0.662
0.985ValMet: 0.985 ± 0.268
3.449ValAsn: 3.449 ± 0.388
1.56ValPro: 1.56 ± 0.387
2.135ValGln: 2.135 ± 0.348
2.135ValArg: 2.135 ± 0.563
5.419ValSer: 5.419 ± 0.825
5.091ValThr: 5.091 ± 0.888
4.844ValVal: 4.844 ± 0.519
1.15ValTrp: 1.15 ± 0.345
2.299ValTyr: 2.299 ± 0.504
0.0ValXaa: 0.0 ± 0.0
Trp
0.575TrpAla: 0.575 ± 0.192
0.164TrpCys: 0.164 ± 0.131
0.328TrpAsp: 0.328 ± 0.147
0.903TrpGlu: 0.903 ± 0.326
0.657TrpPhe: 0.657 ± 0.245
0.657TrpGly: 0.657 ± 0.247
0.164TrpHis: 0.164 ± 0.118
0.328TrpIle: 0.328 ± 0.142
0.821TrpLys: 0.821 ± 0.24
0.985TrpLeu: 0.985 ± 0.291
0.082TrpMet: 0.082 ± 0.082
1.232TrpAsn: 1.232 ± 0.736
0.0TrpPro: 0.0 ± 0.0
0.657TrpGln: 0.657 ± 0.235
0.328TrpArg: 0.328 ± 0.151
1.15TrpSer: 1.15 ± 0.333
0.903TrpThr: 0.903 ± 0.312
0.575TrpVal: 0.575 ± 0.191
0.328TrpTrp: 0.328 ± 0.145
0.575TrpTyr: 0.575 ± 0.236
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.299TyrAla: 2.299 ± 0.453
0.411TyrCys: 0.411 ± 0.179
3.941TyrAsp: 3.941 ± 0.782
2.71TyrGlu: 2.71 ± 0.494
1.478TyrPhe: 1.478 ± 0.31
2.217TyrGly: 2.217 ± 0.608
0.411TyrHis: 0.411 ± 0.189
3.695TyrIle: 3.695 ± 0.506
2.627TyrLys: 2.627 ± 0.426
3.12TyrLeu: 3.12 ± 0.494
1.724TyrMet: 1.724 ± 0.327
2.381TyrAsn: 2.381 ± 0.386
1.232TyrPro: 1.232 ± 0.403
1.642TyrGln: 1.642 ± 0.444
1.971TyrArg: 1.971 ± 0.44
3.531TyrSer: 3.531 ± 0.533
2.381TyrThr: 2.381 ± 0.489
2.217TyrVal: 2.217 ± 0.421
0.493TyrTrp: 0.493 ± 0.205
1.888TyrTyr: 1.888 ± 0.436
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 55 proteins (12180 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski