Exophiala aquamarina CBS 119918
Average proteome isoelectric point is 6.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13118 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A072Q243|A0A072Q243_9EURO DNA excision repair protein ERCC-4 OS=Exophiala aquamarina CBS 119918 OX=1182545 GN=A1O9_03532 PE=3 SV=1
MM1 pKa = 7.33 VPQSLLGLLSLPLLVSAHH19 pKa = 5.39 FHH21 pKa = 5.47 MLYY24 pKa = 7.22 PTPRR28 pKa = 11.84 SDD30 pKa = 5.95 DD31 pKa = 4.14 DD32 pKa = 3.67 DD33 pKa = 4.21 TEE35 pKa = 4.28 PTFPCGGFPVSDD47 pKa = 3.15 NRR49 pKa = 11.84 TAVSSTAFPLALEE62 pKa = 4.47 MGHH65 pKa = 6.92 DD66 pKa = 3.3 RR67 pKa = 11.84 TVIQVLLGLGNNVGDD82 pKa = 4.71 GFDD85 pKa = 3.2 ITLKK89 pKa = 9.18 QTFQQQGEE97 pKa = 4.56 GAFCLPSVQIPSGLNITDD115 pKa = 3.7 GTNGTLQVVTDD126 pKa = 4.11 GEE128 pKa = 4.82 GGGGLYY134 pKa = 9.7 ICADD138 pKa = 3.83 LTFTSSPTVQMLPSSCANGTGITAFPLSSNVNANQTNGDD177 pKa = 3.85 GSPQGGSGSASPSGSGSSTSSSTATGASASPTSNAAILTAGWSLLGAVVLGAVALMM233 pKa = 4.52
Molecular weight: 23.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.745
IPC2_protein 3.923
IPC_protein 3.884
Toseland 3.668
ProMoST 4.075
Dawson 3.897
Bjellqvist 4.062
Wikipedia 3.872
Rodwell 3.719
Grimsley 3.579
Solomon 3.884
Lehninger 3.846
Nozaki 4.024
DTASelect 4.291
Thurlkill 3.745
EMBOSS 3.872
Sillero 4.024
Patrickios 0.896
IPC_peptide 3.884
IPC2_peptide 3.986
IPC2.peptide.svr19 3.894
Protein with the highest isoelectric point:
>tr|A0A072NYA7|A0A072NYA7_9EURO Uncharacterized protein OS=Exophiala aquamarina CBS 119918 OX=1182545 GN=A1O9_11633 PE=4 SV=1
MM1 pKa = 7.07 TGRR4 pKa = 11.84 RR5 pKa = 11.84 APRR8 pKa = 11.84 HH9 pKa = 5.71 LPSLSKK15 pKa = 10.93 LPLSGTTGRR24 pKa = 11.84 KK25 pKa = 7.83 VLRR28 pKa = 11.84 FLNLLRR34 pKa = 11.84 PRR36 pKa = 11.84 RR37 pKa = 11.84 HH38 pKa = 5.79 NNLLKK43 pKa = 10.74 GGMIGKK49 pKa = 8.88 SRR51 pKa = 11.84 RR52 pKa = 11.84 PRR54 pKa = 11.84 HH55 pKa = 5.83 LNLSKK60 pKa = 10.76 LPNPPYY66 pKa = 10.04 PNRR69 pKa = 11.84 LRR71 pKa = 11.84 AGTIGNSRR79 pKa = 11.84 PRR81 pKa = 11.84 LPSPPRR87 pKa = 11.84 LPSLLLLNSLKK98 pKa = 11.05 DD99 pKa = 3.5 GMTGNSRR106 pKa = 11.84 PRR108 pKa = 11.84 LPSQFF113 pKa = 3.51
Molecular weight: 12.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.445
IPC2_protein 11.082
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.34
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.062
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.107
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
13118
0
13118
6064173
37
4877
462.3
51.17
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.573 ± 0.017
1.206 ± 0.008
5.61 ± 0.016
5.877 ± 0.017
3.879 ± 0.014
6.89 ± 0.018
2.453 ± 0.009
5.182 ± 0.016
4.731 ± 0.015
9.185 ± 0.021
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.138 ± 0.009
3.729 ± 0.01
5.803 ± 0.025
4.086 ± 0.015
5.92 ± 0.016
8.232 ± 0.023
6.022 ± 0.015
6.164 ± 0.015
1.504 ± 0.009
2.814 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here