Helicobacter apodemus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Epsilonproteobacteria; Campylobacterales; Helicobacteraceae; Helicobacter

Average proteome isoelectric point is 6.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2029 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4U8UJ10|A0A4U8UJ10_9HELI 4-hydroxy-tetrahydrodipicolinate reductase OS=Helicobacter apodemus OX=135569 GN=dapB PE=3 SV=1
MM1 pKa = 7.86DD2 pKa = 5.67WINFNDD8 pKa = 4.01MPDD11 pKa = 4.83FIADD15 pKa = 3.47MEE17 pKa = 4.9NILKK21 pKa = 10.32RR22 pKa = 11.84EE23 pKa = 3.96NLSVTEE29 pKa = 4.04EE30 pKa = 4.32EE31 pKa = 3.99VWEE34 pKa = 4.17IARR37 pKa = 11.84EE38 pKa = 4.39SPCVPLFQNIYY49 pKa = 8.63IEE51 pKa = 4.29LVFMHH56 pKa = 6.98LEE58 pKa = 4.14SLCEE62 pKa = 3.95SHH64 pKa = 6.98NLSCEE69 pKa = 4.06YY70 pKa = 10.3QVNCLASSFSIEE82 pKa = 4.19DD83 pKa = 3.45EE84 pKa = 4.14EE85 pKa = 6.37FYY87 pKa = 11.09TEE89 pKa = 4.5DD90 pKa = 4.3DD91 pKa = 4.17YY92 pKa = 12.1YY93 pKa = 11.75DD94 pKa = 4.93KK95 pKa = 10.81IRR97 pKa = 11.84KK98 pKa = 8.46SAA100 pKa = 3.18

Molecular weight: 11.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A099UHN6|A0A099UHN6_9HELI 10 kDa chaperonin OS=Helicobacter apodemus OX=135569 GN=groS PE=3 SV=1
MM1 pKa = 7.28KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.04QPHH8 pKa = 4.86NTPRR12 pKa = 11.84KK13 pKa = 7.32RR14 pKa = 11.84THH16 pKa = 6.02GFRR19 pKa = 11.84ARR21 pKa = 11.84MQNKK25 pKa = 8.76NGRR28 pKa = 11.84RR29 pKa = 11.84IINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.99GRR39 pKa = 11.84KK40 pKa = 8.85KK41 pKa = 10.48LALL44 pKa = 3.91

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2029

0

2029

586621

30

2885

289.1

32.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.359 ± 0.053

1.215 ± 0.023

4.581 ± 0.036

6.968 ± 0.056

5.415 ± 0.047

5.993 ± 0.058

1.715 ± 0.023

8.991 ± 0.048

8.513 ± 0.058

10.811 ± 0.072

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.195 ± 0.025

5.717 ± 0.057

3.241 ± 0.031

3.89 ± 0.038

3.454 ± 0.034

6.552 ± 0.038

4.554 ± 0.047

5.176 ± 0.052

0.766 ± 0.019

3.894 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski