Celeribacter marinus
Average proteome isoelectric point is 6.2
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3134 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0P0A1A4|A0A0P0A1A4_9RHOB Uncharacterized protein OS=Celeribacter marinus OX=1397108 GN=IMCC12053_16 PE=4 SV=1
MM1 pKa = 7.24 ATYY4 pKa = 10.3 ILKK7 pKa = 10.63 GYY9 pKa = 9.25 AASSLSSTSGNLTAQVGHH27 pKa = 6.04 VWRR30 pKa = 11.84 LDD32 pKa = 3.78 PSWDD36 pKa = 3.44 NSNDD40 pKa = 3.1 HH41 pKa = 6.28 LTYY44 pKa = 10.38 TINDD48 pKa = 3.61 DD49 pKa = 3.77 DD50 pKa = 4.14 VRR52 pKa = 11.84 FGGDD56 pKa = 2.97 ATINEE61 pKa = 4.36 VGDD64 pKa = 5.1 DD65 pKa = 3.85 FTQTAVVTNVSGGTVASGGVYY86 pKa = 10.65 LEE88 pKa = 4.37 DD89 pKa = 4.83 QITLSDD95 pKa = 3.73 GQGNLFDD102 pKa = 4.23 VYY104 pKa = 10.79 SVEE107 pKa = 4.21 IGGTQVGVVSNPEE120 pKa = 3.94 LVPGVTYY127 pKa = 10.37 QVSSVSNVSAFNLPDD142 pKa = 3.87 YY143 pKa = 11.47 VDD145 pKa = 4.0 MATPTYY151 pKa = 10.86 DD152 pKa = 3.66 PDD154 pKa = 4.22 LAQTYY159 pKa = 10.85 NGGAYY164 pKa = 9.96 NDD166 pKa = 4.44 EE167 pKa = 4.26 IVAGAGDD174 pKa = 4.55 DD175 pKa = 4.37 IINAGAGADD184 pKa = 3.99 SIEE187 pKa = 4.41 GGAGNDD193 pKa = 3.52 TIYY196 pKa = 10.9 YY197 pKa = 6.62 GTGGATQADD206 pKa = 4.5 GDD208 pKa = 4.36 TVYY211 pKa = 11.07 GGDD214 pKa = 4.1 GDD216 pKa = 6.2 DD217 pKa = 5.14 IIDD220 pKa = 4.32 DD221 pKa = 3.93 EE222 pKa = 5.68 AVVQHH227 pKa = 6.56 NYY229 pKa = 10.99 DD230 pKa = 3.37 DD231 pKa = 3.96 TLYY234 pKa = 11.16 GGAGNDD240 pKa = 3.5 TIYY243 pKa = 11.09 AGGGDD248 pKa = 3.83 DD249 pKa = 4.97 EE250 pKa = 5.63 VYY252 pKa = 11.35 GDD254 pKa = 5.47 ADD256 pKa = 3.68 NDD258 pKa = 4.39 WIHH261 pKa = 6.97 GEE263 pKa = 4.11 GGHH266 pKa = 7.17 DD267 pKa = 3.76 EE268 pKa = 4.29 LHH270 pKa = 6.72 GGTGDD275 pKa = 3.46 DD276 pKa = 4.15 TIYY279 pKa = 11.24 GGDD282 pKa = 4.04 GYY284 pKa = 11.24 DD285 pKa = 5.61 LIWGDD290 pKa = 4.65 DD291 pKa = 4.11 GNDD294 pKa = 3.31 TLYY297 pKa = 11.27 GGNDD301 pKa = 3.76 DD302 pKa = 3.7 DD303 pKa = 4.98 TIYY306 pKa = 11.07 GGAGIDD312 pKa = 3.75 VLYY315 pKa = 11.07 GDD317 pKa = 5.3 AGNDD321 pKa = 3.73 TLSGDD326 pKa = 3.35 AGGDD330 pKa = 3.36 HH331 pKa = 7.45 LYY333 pKa = 11.15 GGTGNDD339 pKa = 3.42 TMSGGTGNDD348 pKa = 2.65 SFYY351 pKa = 11.46 VLEE354 pKa = 4.81 GDD356 pKa = 4.3 GTSTMYY362 pKa = 11.24 GDD364 pKa = 5.56 DD365 pKa = 4.0 DD366 pKa = 4.15 WDD368 pKa = 4.08 TVDD371 pKa = 4.65 FAYY374 pKa = 9.53 STGSAVDD381 pKa = 3.43 VTYY384 pKa = 11.18 SGDD387 pKa = 3.41 NAFNFAFDD395 pKa = 3.96 TGNSSGSGNSMEE407 pKa = 4.68 NFILTGQNDD416 pKa = 4.05 TVDD419 pKa = 3.63 ASLDD423 pKa = 3.73 ATGATYY429 pKa = 10.66 DD430 pKa = 4.44 LRR432 pKa = 11.84 AGDD435 pKa = 3.89 DD436 pKa = 3.71 TFTGGSGNDD445 pKa = 3.14 AVYY448 pKa = 10.61 GGDD451 pKa = 3.63 GADD454 pKa = 4.08 TITLGAGGDD463 pKa = 3.93 YY464 pKa = 11.39 AEE466 pKa = 5.31 GGAGNDD472 pKa = 4.05 TIYY475 pKa = 11.44 GDD477 pKa = 4.85 AGEE480 pKa = 4.48 DD481 pKa = 3.51 WIEE484 pKa = 4.24 GGTGNDD490 pKa = 3.32 TLYY493 pKa = 11.28 GGDD496 pKa = 3.73 DD497 pKa = 3.94 ADD499 pKa = 3.72 TFFYY503 pKa = 10.99 SDD505 pKa = 2.85 GWGSDD510 pKa = 3.4 TVFGGAGISDD520 pKa = 4.57 ADD522 pKa = 3.78 TLDD525 pKa = 3.7 FSNVTTDD532 pKa = 3.25 VTVTFSDD539 pKa = 3.89 WEE541 pKa = 4.71 DD542 pKa = 3.39 GTATSGADD550 pKa = 2.98 SVNFDD555 pKa = 3.95 NIEE558 pKa = 4.4 AIRR561 pKa = 11.84 GGSGADD567 pKa = 3.73 TIDD570 pKa = 3.79 ASPDD574 pKa = 3.31 GSGLLLDD581 pKa = 5.1 GGDD584 pKa = 4.12 GGDD587 pKa = 3.91 TIIGGSGDD595 pKa = 3.73 DD596 pKa = 3.85 EE597 pKa = 5.88 LYY599 pKa = 11.23 GGDD602 pKa = 4.42 GVDD605 pKa = 3.68 TLFGGAGNDD614 pKa = 3.98 AIDD617 pKa = 4.19 GGRR620 pKa = 11.84 GDD622 pKa = 3.86 DD623 pKa = 4.44 TITGGAGDD631 pKa = 4.12 DD632 pKa = 4.62 TITSSNGQDD641 pKa = 2.75 TMVFADD647 pKa = 4.44 GFGNDD652 pKa = 4.39 IITDD656 pKa = 4.3 FDD658 pKa = 4.07 FTDD661 pKa = 4.05 SNSDD665 pKa = 3.35 GYY667 pKa = 9.63 TDD669 pKa = 4.16 DD670 pKa = 3.94 QLDD673 pKa = 3.65 VSALTDD679 pKa = 3.47 SDD681 pKa = 4.3 GNPVNAWDD689 pKa = 4.33 VVVSDD694 pKa = 5.17 DD695 pKa = 4.01 GLGNAVLTFPNGEE708 pKa = 4.57 SITLTGIAPTQVDD721 pKa = 4.55 GAQQMHH727 pKa = 7.52 AIGIPCFVSGTRR739 pKa = 11.84 IMTPSGNRR747 pKa = 11.84 LIEE750 pKa = 4.09 EE751 pKa = 4.4 LRR753 pKa = 11.84 HH754 pKa = 5.69 GDD756 pKa = 4.05 LVSTLNSGAQPILWAGRR773 pKa = 11.84 TTLKK777 pKa = 10.35 RR778 pKa = 11.84 ARR780 pKa = 11.84 LALQPDD786 pKa = 4.75 LLPIRR791 pKa = 11.84 IRR793 pKa = 11.84 DD794 pKa = 3.75 GTLGNSGDD802 pKa = 3.7 LWVSPQHH809 pKa = 6.59 GLVLPDD815 pKa = 4.03 LCSHH819 pKa = 6.81 AKK821 pKa = 10.45 AGVLVRR827 pKa = 11.84 AKK829 pKa = 10.41 HH830 pKa = 5.46 LLEE833 pKa = 5.93 HH834 pKa = 6.59 GDD836 pKa = 3.36 GRR838 pKa = 11.84 VRR840 pKa = 11.84 RR841 pKa = 11.84 AKK843 pKa = 10.23 GSRR846 pKa = 11.84 DD847 pKa = 3.25 VTYY850 pKa = 10.73 HH851 pKa = 6.4 HH852 pKa = 7.25 LLLPRR857 pKa = 11.84 HH858 pKa = 5.86 ALILANGCASEE869 pKa = 4.26 SLFPGRR875 pKa = 11.84 FALSGFDD882 pKa = 3.19 RR883 pKa = 11.84 AAKK886 pKa = 10.62 AEE888 pKa = 4.1 LFDD891 pKa = 5.35 LFPALEE897 pKa = 4.51 TILRR901 pKa = 11.84 SGAPATDD908 pKa = 3.61 AARR911 pKa = 11.84 MYY913 pKa = 8.49 GQPVLRR919 pKa = 11.84 FAKK922 pKa = 10.19 GRR924 pKa = 11.84 DD925 pKa = 2.85 WRR927 pKa = 11.84 AGHH930 pKa = 6.19 IRR932 pKa = 11.84 LPKK935 pKa = 9.96 RR936 pKa = 11.84 AA937 pKa = 3.85
Molecular weight: 96.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.682
IPC2_protein 3.567
IPC_protein 3.643
Toseland 3.389
ProMoST 3.834
Dawson 3.656
Bjellqvist 3.808
Wikipedia 3.63
Rodwell 3.452
Grimsley 3.287
Solomon 3.656
Lehninger 3.617
Nozaki 3.757
DTASelect 4.101
Thurlkill 3.452
EMBOSS 3.643
Sillero 3.77
Patrickios 1.227
IPC_peptide 3.643
IPC2_peptide 3.745
IPC2.peptide.svr19 3.702
Protein with the highest isoelectric point:
>tr|A0A0P0ADR4|A0A0P0ADR4_9RHOB Sorbitol-6-phosphate 2-dehydrogenase OS=Celeribacter marinus OX=1397108 GN=IMCC12053_2686 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 KK29 pKa = 8.54 ILNARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.07 SLSAA44 pKa = 3.93
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3134
0
3134
909897
37
2082
290.3
31.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.03 ± 0.062
0.882 ± 0.014
6.405 ± 0.051
5.571 ± 0.042
3.811 ± 0.027
8.413 ± 0.046
2.114 ± 0.024
5.556 ± 0.033
3.68 ± 0.037
9.518 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.931 ± 0.024
2.837 ± 0.024
4.589 ± 0.034
3.194 ± 0.024
6.073 ± 0.041
5.471 ± 0.035
5.894 ± 0.037
7.476 ± 0.033
1.284 ± 0.018
2.272 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here