Serratia phage SM9-3Y

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Teetrevirus; Serratia virus SM9-3Y

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I9S3T5|A0A1I9S3T5_9CAUD Major capsid protein OS=Serratia phage SM9-3Y OX=1897434 PE=3 SV=1
MM1 pKa = 7.7AEE3 pKa = 4.14SNADD7 pKa = 3.26VYY9 pKa = 11.77ASFGVNNAVMTGSTPTEE26 pKa = 4.18HH27 pKa = 6.27EE28 pKa = 4.14QNMLSLDD35 pKa = 3.43VAARR39 pKa = 11.84DD40 pKa = 3.57GDD42 pKa = 4.04DD43 pKa = 5.11AIVLSEE49 pKa = 4.67EE50 pKa = 4.33PSSHH54 pKa = 6.95NDD56 pKa = 3.06DD57 pKa = 4.45PYY59 pKa = 11.73AAGVDD64 pKa = 3.74PFADD68 pKa = 4.11GEE70 pKa = 4.21DD71 pKa = 3.6DD72 pKa = 3.43EE73 pKa = 6.17GRR75 pKa = 11.84IQVRR79 pKa = 11.84ISEE82 pKa = 4.79DD83 pKa = 3.29GNEE86 pKa = 4.1AEE88 pKa = 5.55FDD90 pKa = 3.68TGSDD94 pKa = 3.21NAEE97 pKa = 3.88VEE99 pKa = 4.69TEE101 pKa = 4.07GEE103 pKa = 3.94AAEE106 pKa = 4.45FEE108 pKa = 4.49PLGDD112 pKa = 3.79TPEE115 pKa = 4.39EE116 pKa = 3.88LSQVTEE122 pKa = 3.53QLGQHH127 pKa = 5.61EE128 pKa = 5.22EE129 pKa = 4.43GFQAMVEE136 pKa = 4.22QAVEE140 pKa = 4.12RR141 pKa = 11.84GLSAEE146 pKa = 4.15SVTRR150 pKa = 11.84IYY152 pKa = 10.94EE153 pKa = 3.95EE154 pKa = 4.46YY155 pKa = 9.5EE156 pKa = 3.62ADD158 pKa = 4.46GISEE162 pKa = 3.98KK163 pKa = 10.89SYY165 pKa = 11.82AEE167 pKa = 4.14LEE169 pKa = 3.96AAGYY173 pKa = 10.17SRR175 pKa = 11.84AFVDD179 pKa = 4.73SYY181 pKa = 11.66ISGQEE186 pKa = 3.89ALVDD190 pKa = 3.44QYY192 pKa = 11.99VNQVVAFAGGKK203 pKa = 8.68EE204 pKa = 4.09RR205 pKa = 11.84FSAIHH210 pKa = 5.15THH212 pKa = 7.31LEE214 pKa = 3.98ATNPAAAEE222 pKa = 4.08SLEE225 pKa = 4.12TAMMNRR231 pKa = 11.84DD232 pKa = 3.64LATVKK237 pKa = 10.78AIINLAGEE245 pKa = 4.59SYY247 pKa = 9.79TKK249 pKa = 10.83KK250 pKa = 10.34FGKK253 pKa = 8.89PANRR257 pKa = 11.84SVTKK261 pKa = 10.23RR262 pKa = 11.84ATPVKK267 pKa = 9.56PVARR271 pKa = 11.84QKK273 pKa = 11.17EE274 pKa = 4.53GFTNQAEE281 pKa = 4.64MIKK284 pKa = 10.66AMSDD288 pKa = 2.8PRR290 pKa = 11.84YY291 pKa = 10.05RR292 pKa = 11.84SDD294 pKa = 2.88AAYY297 pKa = 10.33RR298 pKa = 11.84QMVEE302 pKa = 3.81QKK304 pKa = 10.93VIDD307 pKa = 3.65SSFF310 pKa = 2.9

Molecular weight: 33.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1P8EDP1|A0A1P8EDP1_9CAUD Uncharacterized protein OS=Serratia phage SM9-3Y OX=1897434 PE=4 SV=1
MM1 pKa = 7.89RR2 pKa = 11.84LHH4 pKa = 7.03FNTSNGIFSVRR15 pKa = 11.84RR16 pKa = 11.84EE17 pKa = 4.02DD18 pKa = 3.47RR19 pKa = 11.84STAVAYY25 pKa = 8.29EE26 pKa = 4.22RR27 pKa = 11.84NAKK30 pKa = 10.13LPLIGSVVPLSPRR43 pKa = 11.84VHH45 pKa = 7.13LLITRR50 pKa = 11.84GEE52 pKa = 4.31FIKK55 pKa = 10.9AMNKK59 pKa = 8.58EE60 pKa = 4.2RR61 pKa = 11.84PHH63 pKa = 8.07LEE65 pKa = 3.6AVVTYY70 pKa = 8.64WPRR73 pKa = 11.84IRR75 pKa = 11.84LFFKK79 pKa = 9.57WVKK82 pKa = 10.03EE83 pKa = 4.04VLL85 pKa = 3.61

Molecular weight: 10.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

48

0

48

11806

37

1320

246.0

27.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.487 ± 0.444

0.906 ± 0.159

6.158 ± 0.231

7.217 ± 0.386

3.702 ± 0.218

7.581 ± 0.364

1.957 ± 0.183

5.319 ± 0.181

6.7 ± 0.352

7.971 ± 0.373

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.795 ± 0.24

4.515 ± 0.314

3.685 ± 0.199

4.032 ± 0.281

5.988 ± 0.245

5.675 ± 0.367

5.192 ± 0.244

6.293 ± 0.285

1.584 ± 0.18

3.244 ± 0.143
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski