Amino acid dipepetide frequency for Serratia phage SM9-3Y

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.843AlaAla: 10.843 ± 1.015
0.762AlaCys: 0.762 ± 0.239
5.337AlaAsp: 5.337 ± 0.745
6.099AlaGlu: 6.099 ± 0.834
3.558AlaPhe: 3.558 ± 0.508
7.624AlaGly: 7.624 ± 0.951
1.694AlaHis: 1.694 ± 0.375
4.998AlaIle: 4.998 ± 0.499
6.523AlaLys: 6.523 ± 0.693
7.878AlaLeu: 7.878 ± 0.661
3.134AlaMet: 3.134 ± 0.58
4.574AlaAsn: 4.574 ± 0.493
2.457AlaPro: 2.457 ± 0.448
4.32AlaGln: 4.32 ± 0.666
5.337AlaArg: 5.337 ± 0.71
5.337AlaSer: 5.337 ± 0.843
3.897AlaThr: 3.897 ± 0.728
5.93AlaVal: 5.93 ± 0.603
1.609AlaTrp: 1.609 ± 0.321
2.965AlaTyr: 2.965 ± 0.572
0.0AlaXaa: 0.0 ± 0.0
Cys
0.678CysAla: 0.678 ± 0.27
0.169CysCys: 0.169 ± 0.104
0.593CysAsp: 0.593 ± 0.194
0.424CysGlu: 0.424 ± 0.195
0.508CysPhe: 0.508 ± 0.242
0.508CysGly: 0.508 ± 0.2
0.424CysHis: 0.424 ± 0.223
0.678CysIle: 0.678 ± 0.244
0.678CysLys: 0.678 ± 0.333
0.762CysLeu: 0.762 ± 0.247
0.0CysMet: 0.0 ± 0.0
0.339CysAsn: 0.339 ± 0.181
0.339CysPro: 0.339 ± 0.158
0.508CysGln: 0.508 ± 0.248
0.678CysArg: 0.678 ± 0.252
0.508CysSer: 0.508 ± 0.217
0.085CysThr: 0.085 ± 0.085
0.508CysVal: 0.508 ± 0.211
0.254CysTrp: 0.254 ± 0.168
0.424CysTyr: 0.424 ± 0.231
0.0CysXaa: 0.0 ± 0.0
Asp
5.421AspAla: 5.421 ± 0.616
0.678AspCys: 0.678 ± 0.269
4.151AspAsp: 4.151 ± 0.536
4.49AspGlu: 4.49 ± 0.75
2.711AspPhe: 2.711 ± 0.413
6.099AspGly: 6.099 ± 0.859
1.186AspHis: 1.186 ± 0.288
2.457AspIle: 2.457 ± 0.517
4.49AspLys: 4.49 ± 0.641
3.981AspLeu: 3.981 ± 0.646
2.287AspMet: 2.287 ± 0.481
2.626AspAsn: 2.626 ± 0.368
3.219AspPro: 3.219 ± 0.463
1.864AspGln: 1.864 ± 0.446
3.05AspArg: 3.05 ± 0.583
2.711AspSer: 2.711 ± 0.53
3.558AspThr: 3.558 ± 0.473
3.897AspVal: 3.897 ± 0.534
0.847AspTrp: 0.847 ± 0.23
1.864AspTyr: 1.864 ± 0.366
0.0AspXaa: 0.0 ± 0.0
Glu
7.539GluAla: 7.539 ± 0.772
0.678GluCys: 0.678 ± 0.251
4.744GluAsp: 4.744 ± 0.613
4.744GluGlu: 4.744 ± 0.713
2.88GluPhe: 2.88 ± 0.424
5.083GluGly: 5.083 ± 0.656
1.864GluHis: 1.864 ± 0.376
3.473GluIle: 3.473 ± 0.584
4.235GluLys: 4.235 ± 0.796
6.014GluLeu: 6.014 ± 0.575
2.372GluMet: 2.372 ± 0.504
2.202GluAsn: 2.202 ± 0.384
1.864GluPro: 1.864 ± 0.347
3.897GluGln: 3.897 ± 0.707
4.235GluArg: 4.235 ± 0.491
4.235GluSer: 4.235 ± 0.495
3.219GluThr: 3.219 ± 0.488
4.235GluVal: 4.235 ± 0.536
1.44GluTrp: 1.44 ± 0.39
3.134GluTyr: 3.134 ± 0.38
0.0GluXaa: 0.0 ± 0.0
Phe
2.626PheAla: 2.626 ± 0.39
0.424PheCys: 0.424 ± 0.22
2.88PheAsp: 2.88 ± 0.53
2.118PheGlu: 2.118 ± 0.368
1.101PhePhe: 1.101 ± 0.274
3.304PheGly: 3.304 ± 0.559
0.508PheHis: 0.508 ± 0.203
2.118PheIle: 2.118 ± 0.458
3.05PheLys: 3.05 ± 0.602
2.711PheLeu: 2.711 ± 0.506
1.271PheMet: 1.271 ± 0.324
2.118PheAsn: 2.118 ± 0.383
1.609PhePro: 1.609 ± 0.364
1.44PheGln: 1.44 ± 0.344
1.694PheArg: 1.694 ± 0.291
1.948PheSer: 1.948 ± 0.364
3.05PheThr: 3.05 ± 0.474
2.033PheVal: 2.033 ± 0.337
0.254PheTrp: 0.254 ± 0.124
0.762PheTyr: 0.762 ± 0.201
0.0PheXaa: 0.0 ± 0.0
Gly
6.946GlyAla: 6.946 ± 0.88
1.186GlyCys: 1.186 ± 0.418
5.252GlyAsp: 5.252 ± 0.689
5.76GlyGlu: 5.76 ± 0.614
3.388GlyPhe: 3.388 ± 0.435
5.421GlyGly: 5.421 ± 0.896
1.017GlyHis: 1.017 ± 0.306
4.744GlyIle: 4.744 ± 0.713
5.506GlyLys: 5.506 ± 0.796
6.777GlyLeu: 6.777 ± 0.764
2.202GlyMet: 2.202 ± 0.372
3.643GlyAsn: 3.643 ± 0.693
0.932GlyPro: 0.932 ± 0.272
2.541GlyGln: 2.541 ± 0.468
4.659GlyArg: 4.659 ± 0.509
3.812GlySer: 3.812 ± 0.521
3.388GlyThr: 3.388 ± 0.716
4.32GlyVal: 4.32 ± 0.615
1.609GlyTrp: 1.609 ± 0.43
2.711GlyTyr: 2.711 ± 0.462
0.0GlyXaa: 0.0 ± 0.0
His
1.44HisAla: 1.44 ± 0.375
0.339HisCys: 0.339 ± 0.154
0.762HisAsp: 0.762 ± 0.256
1.186HisGlu: 1.186 ± 0.423
0.932HisPhe: 0.932 ± 0.26
1.525HisGly: 1.525 ± 0.28
0.508HisHis: 0.508 ± 0.175
1.44HisIle: 1.44 ± 0.306
1.271HisLys: 1.271 ± 0.275
2.033HisLeu: 2.033 ± 0.431
0.593HisMet: 0.593 ± 0.2
0.847HisAsn: 0.847 ± 0.219
0.678HisPro: 0.678 ± 0.245
0.085HisGln: 0.085 ± 0.08
1.017HisArg: 1.017 ± 0.267
1.609HisSer: 1.609 ± 0.454
0.847HisThr: 0.847 ± 0.189
1.271HisVal: 1.271 ± 0.283
0.424HisTrp: 0.424 ± 0.159
0.762HisTyr: 0.762 ± 0.214
0.0HisXaa: 0.0 ± 0.0
Ile
4.49IleAla: 4.49 ± 0.543
0.254IleCys: 0.254 ± 0.146
3.219IleAsp: 3.219 ± 0.566
4.659IleGlu: 4.659 ± 0.612
1.101IlePhe: 1.101 ± 0.259
3.643IleGly: 3.643 ± 0.553
1.694IleHis: 1.694 ± 0.305
3.812IleIle: 3.812 ± 0.814
3.643IleLys: 3.643 ± 0.62
3.558IleLeu: 3.558 ± 0.397
0.932IleMet: 0.932 ± 0.275
3.05IleAsn: 3.05 ± 0.625
2.457IlePro: 2.457 ± 0.442
1.948IleGln: 1.948 ± 0.366
3.473IleArg: 3.473 ± 0.541
3.558IleSer: 3.558 ± 0.482
2.372IleThr: 2.372 ± 0.345
2.711IleVal: 2.711 ± 0.409
0.762IleTrp: 0.762 ± 0.306
1.864IleTyr: 1.864 ± 0.29
0.0IleXaa: 0.0 ± 0.0
Lys
7.709LysAla: 7.709 ± 0.736
0.424LysCys: 0.424 ± 0.228
3.219LysAsp: 3.219 ± 0.387
5.252LysGlu: 5.252 ± 0.596
2.541LysPhe: 2.541 ± 0.412
5.506LysGly: 5.506 ± 0.6
1.779LysHis: 1.779 ± 0.371
1.948LysIle: 1.948 ± 0.33
4.235LysLys: 4.235 ± 1.002
5.167LysLeu: 5.167 ± 0.896
1.864LysMet: 1.864 ± 0.342
2.287LysAsn: 2.287 ± 0.382
2.795LysPro: 2.795 ± 0.607
2.88LysGln: 2.88 ± 0.615
4.32LysArg: 4.32 ± 0.618
3.134LysSer: 3.134 ± 0.625
3.134LysThr: 3.134 ± 0.502
5.676LysVal: 5.676 ± 0.629
1.017LysTrp: 1.017 ± 0.306
2.118LysTyr: 2.118 ± 0.294
0.0LysXaa: 0.0 ± 0.0
Leu
7.878LeuAla: 7.878 ± 1.19
0.339LeuCys: 0.339 ± 0.168
4.574LeuAsp: 4.574 ± 0.419
5.845LeuGlu: 5.845 ± 0.855
2.372LeuPhe: 2.372 ± 0.433
4.49LeuGly: 4.49 ± 0.485
1.186LeuHis: 1.186 ± 0.321
4.405LeuIle: 4.405 ± 0.512
6.184LeuLys: 6.184 ± 0.749
4.913LeuLeu: 4.913 ± 0.677
2.202LeuMet: 2.202 ± 0.396
3.727LeuAsn: 3.727 ± 0.494
3.643LeuPro: 3.643 ± 0.54
3.812LeuGln: 3.812 ± 0.505
5.76LeuArg: 5.76 ± 0.608
5.167LeuSer: 5.167 ± 0.704
4.998LeuThr: 4.998 ± 0.576
4.405LeuVal: 4.405 ± 0.622
1.44LeuTrp: 1.44 ± 0.434
2.372LeuTyr: 2.372 ± 0.523
0.0LeuXaa: 0.0 ± 0.0
Met
2.965MetAla: 2.965 ± 0.344
0.339MetCys: 0.339 ± 0.19
1.864MetAsp: 1.864 ± 0.548
1.355MetGlu: 1.355 ± 0.475
1.271MetPhe: 1.271 ± 0.275
2.033MetGly: 2.033 ± 0.455
0.254MetHis: 0.254 ± 0.136
1.355MetIle: 1.355 ± 0.246
0.762MetLys: 0.762 ± 0.229
3.219MetLeu: 3.219 ± 0.484
1.101MetMet: 1.101 ± 0.293
1.609MetAsn: 1.609 ± 0.326
1.355MetPro: 1.355 ± 0.323
1.355MetGln: 1.355 ± 0.389
1.44MetArg: 1.44 ± 0.317
1.186MetSer: 1.186 ± 0.236
1.864MetThr: 1.864 ± 0.379
1.948MetVal: 1.948 ± 0.343
0.085MetTrp: 0.085 ± 0.089
0.593MetTyr: 0.593 ± 0.245
0.0MetXaa: 0.0 ± 0.0
Asn
4.32AsnAla: 4.32 ± 0.742
0.508AsnCys: 0.508 ± 0.257
2.711AsnAsp: 2.711 ± 0.527
2.711AsnGlu: 2.711 ± 0.434
1.609AsnPhe: 1.609 ± 0.364
3.558AsnGly: 3.558 ± 0.49
0.339AsnHis: 0.339 ± 0.164
2.88AsnIle: 2.88 ± 0.579
2.711AsnLys: 2.711 ± 0.409
3.219AsnLeu: 3.219 ± 0.426
0.932AsnMet: 0.932 ± 0.244
2.287AsnAsn: 2.287 ± 0.394
2.795AsnPro: 2.795 ± 0.537
1.864AsnGln: 1.864 ± 0.342
2.965AsnArg: 2.965 ± 0.69
2.457AsnSer: 2.457 ± 0.527
2.033AsnThr: 2.033 ± 0.391
2.287AsnVal: 2.287 ± 0.526
0.847AsnTrp: 0.847 ± 0.286
2.118AsnTyr: 2.118 ± 0.493
0.0AsnXaa: 0.0 ± 0.0
Pro
2.541ProAla: 2.541 ± 0.441
0.339ProCys: 0.339 ± 0.147
2.457ProAsp: 2.457 ± 0.382
4.066ProGlu: 4.066 ± 0.791
1.271ProPhe: 1.271 ± 0.287
2.033ProGly: 2.033 ± 0.373
0.678ProHis: 0.678 ± 0.179
1.609ProIle: 1.609 ± 0.405
2.795ProLys: 2.795 ± 0.568
3.05ProLeu: 3.05 ± 0.564
1.101ProMet: 1.101 ± 0.306
2.372ProAsn: 2.372 ± 0.512
1.101ProPro: 1.101 ± 0.349
1.271ProGln: 1.271 ± 0.318
1.864ProArg: 1.864 ± 0.393
1.948ProSer: 1.948 ± 0.317
1.694ProThr: 1.694 ± 0.305
2.541ProVal: 2.541 ± 0.537
0.762ProTrp: 0.762 ± 0.154
1.355ProTyr: 1.355 ± 0.33
0.0ProXaa: 0.0 ± 0.0
Gln
4.913GlnAla: 4.913 ± 0.839
0.169GlnCys: 0.169 ± 0.117
1.779GlnAsp: 1.779 ± 0.478
3.897GlnGlu: 3.897 ± 0.532
1.525GlnPhe: 1.525 ± 0.33
2.88GlnGly: 2.88 ± 0.393
0.593GlnHis: 0.593 ± 0.155
2.287GlnIle: 2.287 ± 0.435
2.711GlnLys: 2.711 ± 0.498
3.473GlnLeu: 3.473 ± 0.615
0.932GlnMet: 0.932 ± 0.257
1.609GlnAsn: 1.609 ± 0.395
1.525GlnPro: 1.525 ± 0.402
1.694GlnGln: 1.694 ± 0.354
2.118GlnArg: 2.118 ± 0.44
2.033GlnSer: 2.033 ± 0.457
1.864GlnThr: 1.864 ± 0.433
2.457GlnVal: 2.457 ± 0.403
0.847GlnTrp: 0.847 ± 0.258
1.017GlnTyr: 1.017 ± 0.337
0.0GlnXaa: 0.0 ± 0.0
Arg
6.269ArgAla: 6.269 ± 0.891
0.847ArgCys: 0.847 ± 0.226
3.812ArgAsp: 3.812 ± 0.683
4.574ArgGlu: 4.574 ± 0.731
2.711ArgPhe: 2.711 ± 0.416
4.066ArgGly: 4.066 ± 0.651
1.609ArgHis: 1.609 ± 0.373
3.134ArgIle: 3.134 ± 0.631
3.558ArgLys: 3.558 ± 0.543
5.337ArgLeu: 5.337 ± 0.627
2.033ArgMet: 2.033 ± 0.514
2.711ArgAsn: 2.711 ± 0.478
1.609ArgPro: 1.609 ± 0.317
2.033ArgGln: 2.033 ± 0.366
3.304ArgArg: 3.304 ± 0.464
4.659ArgSer: 4.659 ± 0.722
2.795ArgThr: 2.795 ± 0.405
2.795ArgVal: 2.795 ± 0.344
0.847ArgTrp: 0.847 ± 0.263
1.186ArgTyr: 1.186 ± 0.209
0.0ArgXaa: 0.0 ± 0.0
Ser
4.574SerAla: 4.574 ± 0.556
0.424SerCys: 0.424 ± 0.201
4.659SerAsp: 4.659 ± 0.605
3.812SerGlu: 3.812 ± 0.746
2.541SerPhe: 2.541 ± 0.321
4.744SerGly: 4.744 ± 0.607
0.847SerHis: 0.847 ± 0.245
3.304SerIle: 3.304 ± 0.618
3.812SerLys: 3.812 ± 0.598
3.558SerLeu: 3.558 ± 0.446
0.593SerMet: 0.593 ± 0.206
1.779SerAsn: 1.779 ± 0.324
2.033SerPro: 2.033 ± 0.574
1.948SerGln: 1.948 ± 0.376
3.897SerArg: 3.897 ± 0.566
3.727SerSer: 3.727 ± 0.647
3.219SerThr: 3.219 ± 0.362
4.151SerVal: 4.151 ± 0.505
0.593SerTrp: 0.593 ± 0.162
2.541SerTyr: 2.541 ± 0.566
0.0SerXaa: 0.0 ± 0.0
Thr
4.405ThrAla: 4.405 ± 0.604
0.508ThrCys: 0.508 ± 0.235
2.626ThrAsp: 2.626 ± 0.457
3.134ThrGlu: 3.134 ± 0.563
1.864ThrPhe: 1.864 ± 0.377
5.337ThrGly: 5.337 ± 0.782
1.44ThrHis: 1.44 ± 0.246
3.643ThrIle: 3.643 ± 0.495
3.388ThrLys: 3.388 ± 0.369
4.828ThrLeu: 4.828 ± 0.669
1.355ThrMet: 1.355 ± 0.308
1.864ThrAsn: 1.864 ± 0.378
2.626ThrPro: 2.626 ± 0.432
1.525ThrGln: 1.525 ± 0.314
2.118ThrArg: 2.118 ± 0.317
3.219ThrSer: 3.219 ± 0.493
2.965ThrThr: 2.965 ± 0.587
3.219ThrVal: 3.219 ± 0.405
0.339ThrTrp: 0.339 ± 0.135
1.525ThrTyr: 1.525 ± 0.363
0.0ThrXaa: 0.0 ± 0.0
Val
4.913ValAla: 4.913 ± 0.614
0.254ValCys: 0.254 ± 0.139
3.558ValAsp: 3.558 ± 0.405
4.659ValGlu: 4.659 ± 0.545
1.779ValPhe: 1.779 ± 0.437
4.744ValGly: 4.744 ± 0.724
1.017ValHis: 1.017 ± 0.276
2.457ValIle: 2.457 ± 0.456
4.405ValLys: 4.405 ± 0.578
5.337ValLeu: 5.337 ± 0.672
1.271ValMet: 1.271 ± 0.312
3.05ValAsn: 3.05 ± 0.403
2.626ValPro: 2.626 ± 0.468
2.795ValGln: 2.795 ± 0.538
4.235ValArg: 4.235 ± 0.604
3.388ValSer: 3.388 ± 0.467
4.235ValThr: 4.235 ± 0.516
4.998ValVal: 4.998 ± 0.818
1.017ValTrp: 1.017 ± 0.35
2.202ValTyr: 2.202 ± 0.474
0.0ValXaa: 0.0 ± 0.0
Trp
1.186TrpAla: 1.186 ± 0.349
0.339TrpCys: 0.339 ± 0.135
0.762TrpAsp: 0.762 ± 0.227
1.101TrpGlu: 1.101 ± 0.231
0.339TrpPhe: 0.339 ± 0.182
1.017TrpGly: 1.017 ± 0.236
0.424TrpHis: 0.424 ± 0.222
0.424TrpIle: 0.424 ± 0.308
1.271TrpLys: 1.271 ± 0.354
1.44TrpLeu: 1.44 ± 0.373
0.678TrpMet: 0.678 ± 0.218
1.101TrpAsn: 1.101 ± 0.299
0.254TrpPro: 0.254 ± 0.149
0.762TrpGln: 0.762 ± 0.252
1.271TrpArg: 1.271 ± 0.301
0.847TrpSer: 0.847 ± 0.335
0.762TrpThr: 0.762 ± 0.253
1.271TrpVal: 1.271 ± 0.381
0.339TrpTrp: 0.339 ± 0.156
0.254TrpTyr: 0.254 ± 0.125
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.219TyrAla: 3.219 ± 0.45
0.085TyrCys: 0.085 ± 0.088
2.626TyrAsp: 2.626 ± 0.582
2.287TyrGlu: 2.287 ± 0.361
1.017TyrPhe: 1.017 ± 0.343
2.202TyrGly: 2.202 ± 0.422
0.508TyrHis: 0.508 ± 0.195
2.033TyrIle: 2.033 ± 0.444
1.779TyrLys: 1.779 ± 0.331
2.457TyrLeu: 2.457 ± 0.431
1.017TyrMet: 1.017 ± 0.26
1.355TyrAsn: 1.355 ± 0.294
0.932TyrPro: 0.932 ± 0.238
1.779TyrGln: 1.779 ± 0.48
2.457TyrArg: 2.457 ± 0.417
1.271TyrSer: 1.271 ± 0.285
1.948TyrThr: 1.948 ± 0.384
2.287TyrVal: 2.287 ± 0.421
0.508TyrTrp: 0.508 ± 0.215
0.678TyrTyr: 0.678 ± 0.241
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 48 proteins (11806 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski