Streptococcus phage CHPC1041
Average proteome isoelectric point is 6.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3G8FCB7|A0A3G8FCB7_9CAUD Uncharacterized protein OS=Streptococcus phage CHPC1041 OX=2365015 GN=CHPC1041_0032 PE=4 SV=1
MM1 pKa = 7.12 VNWVDD6 pKa = 4.58 KK7 pKa = 11.1 DD8 pKa = 4.24 GNDD11 pKa = 3.97 LPDD14 pKa = 4.77 GADD17 pKa = 3.18 QDD19 pKa = 4.92 FKK21 pKa = 11.67 AGMYY25 pKa = 10.19 FSFAGDD31 pKa = 3.52 EE32 pKa = 4.3 VNITDD37 pKa = 4.03 TGAGGYY43 pKa = 9.6 YY44 pKa = 9.97 GGYY47 pKa = 9.38 YY48 pKa = 9.03 YY49 pKa = 10.88 RR50 pKa = 11.84 KK51 pKa = 9.85 FEE53 pKa = 4.13 FGQFGTVWLSCWNKK67 pKa = 10.66 DD68 pKa = 3.89 DD69 pKa = 5.14 LVNYY73 pKa = 7.38 YY74 pKa = 8.26 QQ75 pKa = 4.22
Molecular weight: 8.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.826
IPC2_protein 3.872
IPC_protein 3.821
Toseland 3.592
ProMoST 4.024
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.834
Rodwell 3.643
Grimsley 3.503
Solomon 3.821
Lehninger 3.783
Nozaki 3.973
DTASelect 4.266
Thurlkill 3.681
EMBOSS 3.846
Sillero 3.948
Patrickios 0.299
IPC_peptide 3.808
IPC2_peptide 3.91
IPC2.peptide.svr19 3.833
Protein with the highest isoelectric point:
>tr|A0A3G8F9K6|A0A3G8F9K6_9CAUD HNHc domain-containing protein OS=Streptococcus phage CHPC1041 OX=2365015 GN=CHPC1041_0023 PE=4 SV=1
MM1 pKa = 7.36 ARR3 pKa = 11.84 VRR5 pKa = 11.84 YY6 pKa = 9.32 LPSDD10 pKa = 3.79 FRR12 pKa = 11.84 YY13 pKa = 9.99 KK14 pKa = 10.95 ADD16 pKa = 3.48 FGTYY20 pKa = 9.38 QSTPNKK26 pKa = 8.16 FTGVSVPKK34 pKa = 10.14 FVKK37 pKa = 10.38 QFTLHH42 pKa = 5.9 YY43 pKa = 9.16 KK44 pKa = 9.46 PHH46 pKa = 6.1 TRR48 pKa = 11.84 TLNQEE53 pKa = 3.69 YY54 pKa = 10.23 LAIQNGEE61 pKa = 3.55 NDD63 pKa = 3.25 TRR65 pKa = 11.84 VIIIRR70 pKa = 11.84 HH71 pKa = 4.69 NAKK74 pKa = 9.97 VLEE77 pKa = 4.4 GQVVVLNGTQYY88 pKa = 11.3 DD89 pKa = 3.78 IVRR92 pKa = 11.84 ISPDD96 pKa = 3.02 EE97 pKa = 3.9 NFGFNHH103 pKa = 6.67 YY104 pKa = 11.11 DD105 pKa = 3.11 FLTLKK110 pKa = 10.2 KK111 pKa = 9.72 RR112 pKa = 11.84 KK113 pKa = 9.51 KK114 pKa = 10.37 VGG116 pKa = 3.08
Molecular weight: 13.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.137
IPC2_protein 9.399
IPC_protein 9.428
Toseland 9.926
ProMoST 9.663
Dawson 10.16
Bjellqvist 9.838
Wikipedia 10.35
Rodwell 10.54
Grimsley 10.248
Solomon 10.189
Lehninger 10.16
Nozaki 9.882
DTASelect 9.838
Thurlkill 9.999
EMBOSS 10.335
Sillero 10.072
Patrickios 9.94
IPC_peptide 10.189
IPC2_peptide 8.39
IPC2.peptide.svr19 8.404
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
51
0
51
11818
41
1559
231.7
26.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.338 ± 0.552
0.626 ± 0.145
6.541 ± 0.33
6.448 ± 0.406
4.163 ± 0.205
6.871 ± 0.471
1.481 ± 0.12
6.761 ± 0.247
8.36 ± 0.48
7.903 ± 0.368
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.369 ± 0.183
6.414 ± 0.245
3.029 ± 0.202
3.935 ± 0.173
4.036 ± 0.248
6.499 ± 0.317
6.16 ± 0.371
6.219 ± 0.234
1.726 ± 0.158
4.121 ± 0.306
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here