Capybara microvirus Cap3_SP_539
Average proteome isoelectric point is 5.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W8T0|A0A4P8W8T0_9VIRU Minor capsid protein OS=Capybara microvirus Cap3_SP_539 OX=2585471 PE=4 SV=1
MM1 pKa = 7.52 EE2 pKa = 6.83 RR3 pKa = 11.84 ITKK6 pKa = 7.76 QTFIPTMGEE15 pKa = 3.85 YY16 pKa = 10.38 NSPKK20 pKa = 9.7 FGVFVEE26 pKa = 4.41 TATYY30 pKa = 10.58 VPAKK34 pKa = 10.81 SMIEE38 pKa = 3.99 SLMLAGEE45 pKa = 4.48 RR46 pKa = 11.84 LRR48 pKa = 11.84 CARR51 pKa = 11.84 AEE53 pKa = 4.13 NYY55 pKa = 10.49 DD56 pKa = 3.62 SDD58 pKa = 4.15 EE59 pKa = 4.24 VEE61 pKa = 4.21 PDD63 pKa = 3.23 FGFDD67 pKa = 2.86 TSVRR71 pKa = 11.84 DD72 pKa = 3.95 FEE74 pKa = 4.45 LSEE77 pKa = 3.98 IGSAISHH84 pKa = 6.54 GALNPQPISQQTVVPEE100 pKa = 4.3 SVPSEE105 pKa = 3.98 ASAEE109 pKa = 4.0 AQIQSS114 pKa = 3.4
Molecular weight: 12.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.463
IPC2_protein 4.406
IPC_protein 4.266
Toseland 4.126
ProMoST 4.368
Dawson 4.202
Bjellqvist 4.355
Wikipedia 4.05
Rodwell 4.101
Grimsley 4.037
Solomon 4.19
Lehninger 4.139
Nozaki 4.317
DTASelect 4.406
Thurlkill 4.126
EMBOSS 4.075
Sillero 4.368
Patrickios 3.757
IPC_peptide 4.202
IPC2_peptide 4.368
IPC2.peptide.svr19 4.284
Protein with the highest isoelectric point:
>tr|A0A4P8W8S3|A0A4P8W8S3_9VIRU Uncharacterized protein OS=Capybara microvirus Cap3_SP_539 OX=2585471 PE=4 SV=1
MM1 pKa = 8.14 DD2 pKa = 4.08 CTFPLTIKK10 pKa = 10.61 NPRR13 pKa = 11.84 TNQFIKK19 pKa = 10.4 VPCGMCIHH27 pKa = 6.43 CRR29 pKa = 11.84 IAKK32 pKa = 9.33 VRR34 pKa = 11.84 DD35 pKa = 2.95 WSLRR39 pKa = 11.84 LIHH42 pKa = 6.91 EE43 pKa = 4.28 NSLWQDD49 pKa = 2.94 SCFITLTYY57 pKa = 10.87 SDD59 pKa = 5.55 DD60 pKa = 3.9 NLPLTNCGKK69 pKa = 8.25 MTLFKK74 pKa = 10.64 PDD76 pKa = 3.4 FQNFMKK82 pKa = 10.69 RR83 pKa = 11.84 LRR85 pKa = 11.84 FRR87 pKa = 11.84 VSSPIKK93 pKa = 10.0 YY94 pKa = 8.43 YY95 pKa = 11.13 ACGEE99 pKa = 4.11 YY100 pKa = 10.53 GDD102 pKa = 4.17 NGNRR106 pKa = 11.84 PHH108 pKa = 5.65 YY109 pKa = 9.78 HH110 pKa = 6.74 AIIFGMSCKK119 pKa = 10.25 DD120 pKa = 3.26 LRR122 pKa = 11.84 DD123 pKa = 5.52 FMICWDD129 pKa = 3.71 KK130 pKa = 11.66 GFVTVKK136 pKa = 9.9 PVNAQRR142 pKa = 11.84 CRR144 pKa = 11.84 YY145 pKa = 7.85 VCGYY149 pKa = 8.41 IQKK152 pKa = 10.55 KK153 pKa = 8.3 IRR155 pKa = 11.84 KK156 pKa = 8.29 NPKK159 pKa = 8.72 EE160 pKa = 3.89 YY161 pKa = 10.4 QSAYY165 pKa = 10.65 GCLQPPFQLMSRR177 pKa = 11.84 GLGLGYY183 pKa = 10.16 LEE185 pKa = 4.92 KK186 pKa = 10.88 NRR188 pKa = 11.84 EE189 pKa = 4.32 EE190 pKa = 4.0 YY191 pKa = 10.11 WLTCRR196 pKa = 11.84 NTLNGVPLSIPRR208 pKa = 11.84 YY209 pKa = 7.6 YY210 pKa = 10.78 LKK212 pKa = 10.52 KK213 pKa = 10.52 DD214 pKa = 3.06 WFLHH218 pKa = 5.11 TKK220 pKa = 10.32 VGLKK224 pKa = 10.05 SDD226 pKa = 4.18 SNPTTEE232 pKa = 4.01 VEE234 pKa = 4.65 SYY236 pKa = 11.33 DD237 pKa = 4.51 SAIQRR242 pKa = 11.84 DD243 pKa = 3.81 KK244 pKa = 11.45 NVRR247 pKa = 11.84 AKK249 pKa = 11.17 NNLKK253 pKa = 10.08 KK254 pKa = 10.58 RR255 pKa = 11.84 KK256 pKa = 9.13 LL257 pKa = 3.52
Molecular weight: 30.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.886
IPC2_protein 8.887
IPC_protein 8.77
Toseland 9.385
ProMoST 9.253
Dawson 9.706
Bjellqvist 9.545
Wikipedia 9.882
Rodwell 9.955
Grimsley 9.765
Solomon 9.736
Lehninger 9.706
Nozaki 9.706
DTASelect 9.443
Thurlkill 9.575
EMBOSS 9.853
Sillero 9.721
Patrickios 4.647
IPC_peptide 9.736
IPC2_peptide 8.492
IPC2.peptide.svr19 7.867
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1329
74
565
265.8
30.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.396 ± 0.997
1.731 ± 0.87
5.117 ± 0.108
5.643 ± 1.034
5.041 ± 0.446
6.321 ± 0.784
1.731 ± 0.329
5.418 ± 0.42
5.719 ± 1.126
7.901 ± 0.716
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.182 ± 0.398
6.998 ± 0.678
4.439 ± 1.137
4.515 ± 0.655
4.59 ± 0.844
8.427 ± 0.997
5.493 ± 0.74
6.095 ± 0.823
1.43 ± 0.316
4.816 ± 0.378
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here