Sulfitobacter sp. CB2047

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Sulfitobacter; unclassified Sulfitobacter

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3563 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A085BTE8|A0A085BTE8_9RHOB Phasin PhaP OS=Sulfitobacter sp. CB2047 OX=1525218 GN=IV89_10805 PE=4 SV=1
MM1 pKa = 7.7KK2 pKa = 10.34FGTSALALCAFLAACGSGTPFNGEE26 pKa = 4.01TEE28 pKa = 4.35ADD30 pKa = 3.47TGTGTDD36 pKa = 3.35GSTGASAIPEE46 pKa = 4.06EE47 pKa = 4.49LANDD51 pKa = 4.46LKK53 pKa = 11.34SFSYY57 pKa = 10.91DD58 pKa = 3.19PASQTLSITGITADD72 pKa = 4.29DD73 pKa = 4.1SEE75 pKa = 4.52FTANYY80 pKa = 9.3RR81 pKa = 11.84RR82 pKa = 11.84RR83 pKa = 11.84PGLDD87 pKa = 2.71RR88 pKa = 11.84NGYY91 pKa = 7.93EE92 pKa = 4.96AYY94 pKa = 8.66TAQDD98 pKa = 3.27GSLDD102 pKa = 3.64RR103 pKa = 11.84HH104 pKa = 4.1VTAYY108 pKa = 10.84VKK110 pKa = 10.74DD111 pKa = 3.55IDD113 pKa = 3.85GTRR116 pKa = 11.84AAVVMTGGQFEE127 pKa = 4.61QVFSGAGYY135 pKa = 10.91SNTSYY140 pKa = 10.71SAPVAPGTQSEE151 pKa = 4.78GGLVTYY157 pKa = 10.27AGNYY161 pKa = 9.05IGLLNGAGSGEE172 pKa = 4.25DD173 pKa = 3.68LAPVADD179 pKa = 4.43GTDD182 pKa = 3.8PDD184 pKa = 4.26PLSRR188 pKa = 11.84QAAEE192 pKa = 4.03VTGKK196 pKa = 10.26VVLTGDD202 pKa = 3.5FTDD205 pKa = 3.84AAVAGIIYY213 pKa = 9.63DD214 pKa = 3.77RR215 pKa = 11.84KK216 pKa = 9.55VTDD219 pKa = 3.87FDD221 pKa = 3.55TGGTYY226 pKa = 10.57DD227 pKa = 4.56PNSATPFEE235 pKa = 4.49AQNIALDD242 pKa = 3.8STGIADD248 pKa = 4.29DD249 pKa = 4.48GSFFGTASQSNNAVGEE265 pKa = 4.05YY266 pKa = 10.7GGIFGGTGATEE277 pKa = 4.06VAGVIHH283 pKa = 7.04AEE285 pKa = 3.69NHH287 pKa = 5.16IALGGSGTPIEE298 pKa = 4.46YY299 pKa = 10.26GAFVLTQCGQPGEE312 pKa = 4.93DD313 pKa = 4.64AICDD317 pKa = 3.56QPEE320 pKa = 3.65RR321 pKa = 4.13

Molecular weight: 32.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A085BTK0|A0A085BTK0_9RHOB Homoserine dehydrogenase OS=Sulfitobacter sp. CB2047 OX=1525218 GN=IV89_11085 PE=3 SV=1
MM1 pKa = 6.96TAIDD5 pKa = 3.97RR6 pKa = 11.84SVMRR10 pKa = 11.84RR11 pKa = 11.84PQWHH15 pKa = 5.36WWVIIPPVLLAALLFGAAIGEE36 pKa = 4.31VSIPPLVVLKK46 pKa = 10.74VLANKK51 pKa = 9.76ALNAGYY57 pKa = 10.3AVDD60 pKa = 5.53KK61 pKa = 10.47IDD63 pKa = 5.64EE64 pKa = 5.12GIVWNYY70 pKa = 9.69RR71 pKa = 11.84MARR74 pKa = 11.84AVVAMCCGAGLALSGVVLQALLRR97 pKa = 11.84NALADD102 pKa = 4.15PYY104 pKa = 11.24LLGISAGASTGAVAVTILGLGGGAISLSIGAFSGALVAFAFVALLAQAAGGGVGLRR160 pKa = 11.84AAGVIVLAGIAGSQMFNALTALMITKK186 pKa = 9.93SANAEE191 pKa = 3.69QARR194 pKa = 11.84AIMFWLLGNMSGVRR208 pKa = 11.84WPDD211 pKa = 3.23VLSALPAAMIGLLACLWHH229 pKa = 6.88ARR231 pKa = 11.84ALDD234 pKa = 3.66AFTFGSDD241 pKa = 2.95SAASLGIPVRR251 pKa = 11.84RR252 pKa = 11.84VQLLLIGTAALVTAVMVSVVGAIGFVGLVIPHH284 pKa = 7.24AMRR287 pKa = 11.84IVVGHH292 pKa = 5.89RR293 pKa = 11.84HH294 pKa = 6.58AILVPASALAGAVFLVFADD313 pKa = 3.67VVSRR317 pKa = 11.84VIVPGQVLPIGVVTALVGAPVFALILIGKK346 pKa = 7.08RR347 pKa = 11.84TSPP350 pKa = 3.55

Molecular weight: 35.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3563

0

3563

1115998

32

1955

313.2

33.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.182 ± 0.049

0.862 ± 0.015

6.335 ± 0.035

5.579 ± 0.038

3.722 ± 0.026

8.568 ± 0.045

2.01 ± 0.022

5.315 ± 0.031

3.471 ± 0.031

9.746 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.934 ± 0.023

2.776 ± 0.021

4.913 ± 0.024

3.451 ± 0.025

6.186 ± 0.041

5.245 ± 0.028

5.764 ± 0.029

7.337 ± 0.034

1.325 ± 0.018

2.279 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski